Hand-held adenosine triphosphate fluorescent detecting instrument

Abstract

The present invention discloses a portable adenosine triphosphate fluorescence detection device comprising a housing consisting of an upper housing and a lower housing, a cover capable of opening/closing and deposited above the housing, a liquid crystal window and a keyboard which are deposited at the upper housing acting as a panel of the device; a circuit board used for performing a fluorescence detection and mounted inside the housing, with a liquid crystal screen, a photodiode, a shielding case and a light trap metal block being mounted thereon, wherein the photodiode is arranged inside the light trap metal block with its the other terminal being connected to a test-tube repository, and a first microswitch and a second microswitch are also mounted at the circuit board. The control circuit comprises a fluorescence detection module, a data acquisition module, a human-computer interface, a power supply module, a control module and the like. Said fluorescence detection module is used for detecting the intensity of fluorescence generated by a reaction of ATP in living microorganism body and fluoresceins and luciferases in the detection reagent, and the data acquisition module is used for converted the fluorescence intensity detected into a digital quantity to ascertain the ATP content, then the presence and content of microorganism is capable of being indirectly ascertained by controlling system analysis and processing and saving data.

Description

technical field [0001] The invention relates to a detection device, especially a handheld adenosine triphosphate fluorescence detection device. The device can be used in food safety and environmental detection departments for on-site rapid detection of microorganisms, rapid screening of large samples, monitoring of key links, and prevention of infection disease, safeguarding public safety, food safety and environmental monitoring. Background technique [0002] ATP (Adenosine Triphosphate), the Chinese name for adenosine triphosphate, exists as an important energy molecule in all organisms from microorganisms to higher animals and plants. In view of the living microorganisms containing ATP, the ATP fluorescence reaction of the following reaction formula can be used to determine the ATP content of the sample by detecting the fluorescence intensity, so that the existence and content of the microorganisms in the sample can be determined indirectly. [00...

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Problems solved by technology

[0006] The traditional method is to use desktop equipment for ATP fluorescence detection of microorganisms. The desktop equipment is large in size and AC power supply, so it cannot be conveniently and flexibly applied to the occasion of on-site rapid detection.

[0007] According to the data retrieval done by the applicant, there is no relevant report on the hand-held ATP fluorescence detector prepared based on the MSP430 ultra-low power consumption microcontroller of TI (Texas Instruments) company in the United States

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