Method for preparing Acaudina leucoprocta collagen oligopeptides by virtue of microwave/ultrasonic assistance and microorganism protease hydrolysis

Abstract

The invention relates to a method for preparing Acaudina leucoprocta collagen oligopeptides by virtue of microwave / ultrasonic assistance and microorganism protease hydrolysis. Collagen peptides are prepared by hydrolyzing East sea Acaudina leucoprocta collagen by virtue of protease produced through local composting of bacillus or bacillus licheniformis or bacillus megaterium, but obtained polypeptides are relatively high in molecular weight and are still difficult to be absorbed by intestinal tracts. A microwave / ultrasonic assisted manner is adopted in the item, so that amino acid residues hidden in a collagen structure can be presented and are hydrolyzed into smaller polypeptides under the action of microorganism protease, and the yield of the oligopeptides (molecular weight is less than 1000Da) can be remarkably increased. The method disclosed by the invention has the beneficial effects that the production cycle is short, the yield is high, the product is high in purity and has remarkable oxidation resistance, good solubility and high absorptivity and has no foreign odor and bitter taste; and the adequate high-value processing and utilization of sea cucumbers are realized.

Description

technical field [0001] The invention relates to the preparation of collagen peptides, in particular to a method for preparing collagen oligopeptides from black ginseng with the aid of microwave / ultrasonic waves and hydrolysis of microbial proteases. Background technique [0002] Collagen polypeptide is a product between protein and amino acid obtained after collagen or gelatin is degraded by protease, etc. Generally, the linear peptide formed by 2 to 10 amino acids through peptide bonds is called oligopeptide or small peptide; A polymer of more than 10 amino acids bonded together is called a polypeptide. In recent years, many scholars have studied the digestion and absorption mechanism of peptides, and believe that peptides and even a considerable amount of proteins can be completely absorbed and utilized by the body in animals, such as oral vaccines and enzyme treatment of diseases. Peptide transport systems are faster than free amino acids. Numerous trials have shown tha...

AI Technical Summary

Problems solved by technology

It is very difficult to remove this layer of dirt. It is difficult for Donghai black ginseng products to meet the national mandatory standard GB2762-2012 "National Standard for Food Safety / Pollution in Food" by conventional cleaning and cleaning with sodium hydroxide and hydrogen peroxide. Requirements of GB2733-2005 "Hygienic Standards for Fresh and Frozen Animal Aquatic Products" and NY / T1514-2007 "Green Food Sea Cucumber and Its Products" standard of the Ministry of Agriculture

From the test results of Donghai black ginseng products collected in the market, the harmful heavy metals exceed the national standard by tens to hundreds of times, which is the fundamental reason why the rich resources of Donghai black ginseng have not been effectively utilized and industrialized