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Preparation method of material for detecting content of dopamine in human body

A technology for detecting materials and dopamine, applied in analytical materials, luminescent materials, material excitation analysis, etc., can solve the problems of expensive instruments, unfavorable discovery of patients, etc., and achieve the effect of low detection limit, excellent fluorescence performance, and good water resistance.

Inactive Publication Date: 2020-05-05
YANTAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(K. Vuorensola, H. Sirén and U. Karjalainenc, J. Chromatogr. B. 2003, 788, 277; A. El-Beqqali, A. Kussak and M. Abdel-Rehim, J. Sep. Sci. 2007, 30, 421.) However, these methods not only require expensive instruments, but also require a long-term oxidant pretreatment process, which is not conducive to quick and easy discovery of the patient's condition

Method used

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  • Preparation method of material for detecting content of dopamine in human body
  • Preparation method of material for detecting content of dopamine in human body
  • Preparation method of material for detecting content of dopamine in human body

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Embodiment 1

[0036] Embodiment 1: Preparation example of Zr-based MOF material.

[0037] This embodiment provides a Zr-based MOF material, the chemical composition of the MOF material is: Zr 6 o 4 (OH) 4 (O 2 C-C 6 h 2 -CO2 (CO 2 h) 2 )·xH 2 O.

[0038] The preparation method of described Zr-based MOF material comprises the steps:

[0039] ZrCl 4 (0.2330g), 1,2,4,5-benzenetetracarboxylic acid (H 4 btec, 0.4321g) and H 2 The mixture of O (5 mL) was stirred into a 25 mL round bottom flask at room temperature, then heated to reflux at 100 °C for 24 hours, centrifuged to obtain a white powder product, which was washed several times with distilled water. To remove organic matter encapsulated in the pores, the product was dispersed in distilled water (about 10 mL per 1 g of product) and heated under reflux for 16 hours. Then, the solid was recovered by centrifugation, washed with ethanol, and dried under vacuum at 60 °C to obtain the final product in 50% yield.

[0040] The obtaine...

Embodiment 2

[0041] Example 2: Tb 3+ -Preparation example of MOF material.

[0042] This example provides a post-utilization synthesis preparation method for obtaining Tb 3+ -MOF material, its specific preparation process is as follows:

[0043] By mixing 100mg of the Zr-based MOF material prepared in Example 1 and TbCl 3 (0.001M) was dispersed in 10mL ethanol at 30°C for 24h to prepare Tb 3+ -MOF materials. Excess Tb was removed by centrifugation and washed several times with ethanol 3+ Afterwards, the obtained white product was vacuum-dried at 60°C for 8 hours to obtain Tb 3+ -MOF materials.

[0044] For the obtained Tb 3+ -MOF materials were characterized by powder X-ray (PXRD) pattern and thermogravimetric (TG) analysis. figure 1 The PXRD pattern of the obtained sample is given, compared with the crystal structure pattern of the Zr-based MOF material, the synthesized Tb 3+ -MOF materials do not undergo changes in crystal structure, indicating that doping with lanthanide elemen...

Embodiment 3

[0045] Embodiment 3: Experimental embodiment of fluorescence sensing.

[0046] This embodiment uses the prepared Tb in embodiment 2 3+ -MOF material for fluorescence sensing experiments, the specific experimental process is as follows:

[0047] 3 mg of Tb 3+ -MOF materials were respectively soaked in plasma (KCl, NaCl, CaCl 2 , MgCl 2 , urea, 1-proline, glucose, NaHCO 3 , DA) and urine (urea, creatinine, KCl, NaCl, DA) chemical substances, after the suspension is formed by ultrasound, the fluorescence spectrum of each suspension is measured.

[0048] The samples prepared in Example 2 were tested for fluorescence spectrum. Figure 6 Recorded Tb 3+ -Characteristic emission spectra of MOFs. Figure 7 The luminescence spectra of mixed suspensions of various substances are given, and it can be seen from the figure that only DA causes a strong fluorescence quenching effect. Figure 8 The fluorescence spectrum of adding DA under the condition of the coexistence of these subst...

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Abstract

The invention relates to a preparation method of a material for detecting the content of dopamine in a human body. The preparation method comprises the following steps: firstly, preparing a Zr-based metal organic framework of which the chemical composition is Zr6O4(OH)4(O2C-C6H2-CO2(CO2H)2).XH2O; and then synthesizing a Tb<3+>-MOF material by utilizing the Zr-based metal organic framework. The prepared Tb<3+>-MOF material shows strong characteristic emission fluorescence of Tb<3+> and good water resistance. The Tb<3+>-MOF material maintains good stability in a pH range of 3.0-10.0, and still maintains a complete crystal structure and fluorescence characteristics after being soaked in an aqueous solution environment for 48h. DA has good concentration dependence and anti-interference performance on the quenching efficiency of fluorescence of the Tb<3+>-MOF material. The detection range of DA is 0-350 [mu] M, and the detection limit is 0.06 [mu] M. In addition, the Tb<3+>-MOF material canbe repeatedly used, and portable test paper is manufactured to be applied to an actual physiological environment. The invention provides a simple and efficient method for detecting the DA in the biological fluid.

Description

technical field [0001] The invention relates to a preparation method of a material for detecting dopamine content in human body. Background technique [0002] The naturally occurring catecholamine dopamine is a neurotransmitter and its hydrochloride salt is used in the treatment of acute congestion and renal failure. Dopamine plays an integral role in controlling the central and peripheral nervous systems. (B.J.Ventonand R.M.Wightman, Anal.Chem.2003,75,414A; K.P.Troyer, M.L.A.V.Heien, B.J.Ventonand R.M.Wightman, Curr.Opin.Chem.Biol.2002,6,696; R.M.Wightman, L.J.May and A.C.Michael, Anal .Chem.1988,60,769A.) However, after the content of dopamine (DA) in the human body is abnormal, it will cause various nervous system disorders, such as schizophrenia, Huntington's disease and Parkinson's disease (PD). Especially when dopamine is severely depleted, the reduced content will increase the incidence of Parkinson's disease. (S. Janezic, S. Threlfell, P.D. Dodson, M.J. Dowie, T.N...

Claims

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Application Information

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IPC IPC(8): C08G83/00C09K11/06G01N21/64
CPCC08G83/008C09K11/06C09K2211/1425C09K2211/182G01N21/6428G01N21/6486G01N2021/6432
Inventor 初瑞清葛凯明闫萌军贺笑春徐志军李大磊
Owner YANTAI UNIV
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