Lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as fluorescent sensor for selectively detecting uric acid and preparation method thereof

A technology of electrospinning and electrospinning technology, which is applied in the field of electrospinning fiber membrane and its preparation for quantitative testing of uric acid, which can solve the problems of complex instruments and limited sensitivity, achieve high sensitivity, improve purity, and quenching efficiency good effect

Active Publication Date: 2019-01-29
WUHAN DGENSEE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Other analytical methods, including HPLC and spectroscopic analysis, suffer from limited sensitivity or complex instrumentation

Method used

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  • Lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as fluorescent sensor for selectively detecting uric acid and preparation method thereof
  • Lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as fluorescent sensor for selectively detecting uric acid and preparation method thereof
  • Lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as fluorescent sensor for selectively detecting uric acid and preparation method thereof

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preparation example Construction

[0045] 1. Preparation of sodium terephthalate brine solution

[0046] Ligand terephthalic acid (referred to as H 2 BDC or PTA) is converted into a water-soluble sodium carboxylic acid salt, and then the sodium carboxylic acid salt ligand and the rare earth center can be used for rapid polymerization in aqueous solution.

[0047]Preparation method: take 1.667g (10mmol) terephthalic acid, add it into 100mL 0.2mol / L sodium hydroxide aqueous solution under stirring, stir at room temperature until the solution is clear, that is, 0.1mol / L sodium terephthalic acid aqueous solution , Reaction equation: 2NaOH+H 2 BDC→Na 2 BDC+2H 2 O.

[0048] 2. Synthesis of Eu-PTA-MOF

[0049] Rapid polymerization in aqueous solution with rare earth elements and sodium terephthalate as basic building blocks.

[0050] Preparation method: Weigh 0.2 mmol europium(III) chloride hexahydrate (EuCl 3 ·6H 2 O) Dissolve in 14mL aqueous solution, stir slowly at room temperature and drop 0.1mol / L Na 2 B...

Embodiment 1

[0057] Preparation of Electrospun Fibrous Membranes Containing Lanthanide Metal-Organic Frameworks

[0058] Preparation of electrospinning solution: weigh 10 mg of Eu-PTA-MOF powder at room temperature, add 2 mL of tetrahydrofuran (THF) / N-methylpyrrolidone (NMP) (3 / 1, v / v) mixed solution to it , Stir at a constant temperature of 30°C until the powder is evenly dispersed, and then ultrasonic for half an hour. Then, a certain amount (accounting for 20% of the mass of the THF / NMP mixture) of the copolymer PMMA / PVP solid was added, and the mixture was stirred at a constant temperature of 30° C. for 4 hours to obtain a milky white suspension solution. It is worth noting that PMMA / PVP is a polymer material, which requires a certain temperature and stirring time during the dissolution process, and the temperature should not be too high, otherwise part of the solvent will volatilize.

[0059] Electrospinning: Since the concentration of spinning solution, electrospinning rate, voltage...

Embodiment 2

[0061] Electrospun fiber membrane anti-interference test

[0062] Due to the presence of other primary metabolites in human urine, such as co-excreted products such as creatinine, creatine, urea, etc., the selectivity of electrospun fibrous membranes containing lanthanide MOFs is critical. In order to investigate the selectivity of electrospun fibrous membranes containing lanthanide metal-organic frameworks for the detection of uric acid, we selected interfering substances including water (H 2 O), Creatinine, Creatine, Urea, SO 4 2- , Na + , K + , NH 4 + , Cl - and Glucose. The aqueous solution of each interfering substance is mixed with uric acid solution (concentration is 0.5mmol / L), joins in the cuvette, then inserts the electrospun fiber membrane prepared in embodiment 1 in the cuvette, and records the fibrous membrane Luminescence spectrum. The influence of various interfering substances in urine on the selectivity of uric acid detected by electrospun fiber membr...

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Abstract

The invention discloses a lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as a fluorescent sensor and a preparation method thereof. The sensor is applied to the quantitative detection of uric acid in human urine. The detection process comprises the following steps of: (I) synthesizing Eu-PTA-MOF powder; (II) synthesizing a copolymer PMMA/PVP; (III) preparing an electrostatic spinning solution; (IV) preparing a nanofiber membrane; (V) cutting the nanofiber membrane; (VI) inserting the cut membrane into a cuvette; and (VII) recording fluorescence spectrum data. The prepared electrostatic spinning fiber membrane shows various kinds of characteristic blue fluorescence of Eu<3+> and good hydrophilicity and shows good fluorescence detection stability at the pH value of 4.5-8.0 in 30 minutes; the quenching efficiency of the uric acid on the electrostatic spinning fiber membrane fluorescence shows good concentration dependence and anti-interference performance; the detection range of the uric acid is 3-100 [mu]M and the detection limit is 1.3 [mu]M; in addition, the electrostatic spinning fiber membrane can be used repeatedly. The invention provides a simple and efficient method for the quantitative detection of the uric acid in biological fluids.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensing and electrospinning, and relates to an electrospun fiber membrane for quantitatively testing uric acid and a preparation method thereof. Background technique [0002] Uric acid (2,6,8-trihydroxypurine, UA) is the end product of purine metabolism in the body. 2 / 3 of uric acid in the human body is excreted through the kidneys with urine, and 1 / 3 is excreted through feces and sweat. Uric acid in body fluids is an important marker molecule for diseases related to purine metabolism, diseases related to uric acid levels in body fluids, leukemia, and pneumonia. [0003] Uric acid functions as an antioxidant in body fluids and may fight oxidative damage in degenerative diseases. However, when the uric acid content in the body exceeds the normal range value, due to the low solubility of uric acid in aqueous solution (about 60mg / L), uric acid may accumulate in the human body, and excessive ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64D01F6/52D01F1/10C08F220/14C08G83/00C08F226/10
CPCC08F220/14C08G83/008D01F1/10D01F6/52G01N21/6428G01N2021/6432C08F226/10
Inventor 肖玉秀郑曲通
Owner WUHAN DGENSEE CO LTD
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