Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution

A technology for synthesizing carbon and mixed solutions, applied in the field of chemical applications, which can solve the problems of high impedance of glass electrodes, sodium error, unsuitable fluorine-containing solutions, etc.

Active Publication Date: 2017-01-11
HUNAN UNIV OF SCI & TECH
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Problems solved by technology

[0003] In the early days, people used the electrochemical method to measure the pH value. However, due to the high impedance of the traditional glass electrode, it is easily damaged, and it is not suitable for the measurement of pH in fluorine-containing solutions, and there is a "sodium error" in a highly alkaline environment. Many problems encountered in pH measurement

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  • Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution
  • Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution
  • Method for quickly synthesizing carbon dots in microwave manner by using yeast bacteria as a main carbon source and using carbon dots to detect pH of solution

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[0024] The following examples are intended to further illustrate the present invention, but not to further limit the present invention.

[0025] (1) Cultivation and extraction of yeast bacteria

[0026] The composition of the medium: glucose 20.0 g, distilled water 1000 mL.

[0027] Accurately weigh 20.0 g of glucose, add it to a beaker containing 900 mL of distilled water, stir with a glass rod to fully dissolve it, and set the volume to 1000 mL. Measure 20 mL of glucose solution into a conical flask, add a cotton plug, and wrap it with kraft paper. Put the glucose solution to be sterilized into a sterilizer, and sterilize at 121°C for 20 min. Place the sterilized glucose solution in a sterile workbench, and turn on the ultraviolet light to sterilize for 2 h. In strict accordance with the aseptic requirements, 0.020 g of Angel High Active Dry Yeast (Ange Yeast Co., Ltd.) was inoculated into the glucose solution, and cultivated at 37 °C for 24 h.

[0028] Take a bottle of ...

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Abstract

The invention belongs to the technical field of chemical application, and particularly relates to a method for quickly synthesizing a carbon dot solution in a microwave manner by using yeast bacteria as a main carbon source and using the carbon dot solution to detect pH of a solution. The method for detecting the pH value of the solution comprises the following step: synthesizing the carbon dot solution by using the yeast bacteria as the main carbon source, using ethylenediamine as an auxiliary carbon source and using water as dispersion liquid under the condition of microwave heating to obtain the carbon dot solution with a fluorescence characteristic. Synthesized carbon dots have the fluorescence characteristic, the maximum excitation wavelength is 390 nm, and the maximum transmitting wavelength is 460 nm; and after the carbon dot solution react with solutions with different pH, the fluorescence intensity value of the carbon dot solution and pH of the solutions are in linear relation as follows: F= 354.66-19.00 pH, and the linear range of detection is 1.83-11.86.

Description

technical field [0001] The invention belongs to the technical field of chemical application, and specifically relates to a carbon dot solution synthesized under microwave heating conditions with yeast bacteria as the main carbon source ethylenediamine as an auxiliary carbon source; the fluorescence of the solution after the carbon dot solution is reacted with solutions of different pH Different values ​​can detect the pH of the solution. Background technique [0002] Fluorescent carbon dots are one of the most popular carbon nanomaterials after fullerenes, carbon nanotubes and graphene. This kind of nanomaterial overcomes some shortcomings of traditional quantum dots, not only has excellent optical properties and small size characteristics, but also has good biocompatibility, and is easy to realize surface functionalization. It is used in biochemical sensing, imaging analysis, and environmental detection. , photocatalytic technology, metal ion detection and drug carrier and...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 龙云飞刘豪敏袁敏莫平高健王星林
Owner HUNAN UNIV OF SCI & TECH
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