A method for rapidly synthesizing carbon dots using yeast bacteria as the main carbon source by microwave and using them for the detection of solution pH

A technology for synthesizing carbon and mixed solutions, which is applied in the field of chemical applications and can solve problems such as unsuitable for fluorine-containing solutions, easy damage, and high impedance of glass electrodes

Active Publication Date: 2019-03-08
HUNAN UNIV OF SCI & TECH
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  • Application Information

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Problems solved by technology

[0003] In the early days, people used the electrochemical method to measure the pH value. However, due to the high impedance of the traditional glass electrode, it is easily damaged, and it is not suitable for the measurement of pH in fluorine-containing solutions, and there is a "sodium error" in a highly alkaline environment. Many problems encountered in pH measurement

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  • A method for rapidly synthesizing carbon dots using yeast bacteria as the main carbon source by microwave and using them for the detection of solution pH
  • A method for rapidly synthesizing carbon dots using yeast bacteria as the main carbon source by microwave and using them for the detection of solution pH
  • A method for rapidly synthesizing carbon dots using yeast bacteria as the main carbon source by microwave and using them for the detection of solution pH

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Embodiment Construction

[0023] The following examples are intended to further illustrate the present invention, but not to further limit the present invention.

[0024] (1) Cultivation and extraction of yeast bacteria

[0025] The composition of the medium: glucose 20.0 g, distilled water 1000 mL.

[0026] Accurately weigh 20.0 g of glucose, add it to a beaker containing 900 mL of distilled water, stir with a glass rod to fully dissolve it, and dilute to 1000 mL. Measure 20 mL of glucose solution into a triangular flask, add a cotton stopper, and wrap it with kraft paper. Put the glucose solution to be sterilized into the sterilization pot and sterilize at 121°C for 20 min. Place the sterilized glucose solution in a sterile workbench and turn on the UV lamp to sterilize for 2 hours. Strictly follow the sterility requirements, inoculate 0.020 g of Angel high-active dry yeast (Angel Yeast Co., Ltd.) into the glucose solution, and finally incubate at 37 °C for 24 h.

[0027] Take a bottle of the above cultur...

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Abstract

The invention belongs to the technical field of chemical application, and particularly relates to a method for quickly synthesizing a carbon dot solution in a microwave manner by using yeast bacteria as a main carbon source and using the carbon dot solution to detect pH of a solution. The method for detecting the pH value of the solution comprises the following step: synthesizing the carbon dot solution by using the yeast bacteria as the main carbon source, using ethylenediamine as an auxiliary carbon source and using water as dispersion liquid under the condition of microwave heating to obtain the carbon dot solution with a fluorescence characteristic. Synthesized carbon dots have the fluorescence characteristic, the maximum excitation wavelength is 390 nm, and the maximum transmitting wavelength is 460 nm; and after the carbon dot solution react with solutions with different pH, the fluorescence intensity value of the carbon dot solution and pH of the solutions are in linear relation as follows: F= 354.66-19.00 pH, and the linear range of detection is 1.83-11.86.

Description

Technical field [0001] The invention belongs to the technical field of chemical application, and specifically relates to a carbon dot solution synthesized under microwave heating conditions with yeast and bacteria as the main carbon source ethylenediamine as an auxiliary carbon source; the fluorescence of the solution after the carbon dot solution reacts with a solution of different pH With different values, the pH of the solution can be detected. Background technique [0002] Fluorescent carbon dots are one of the most popular carbon nanomaterials after fullerene, carbon nanotubes and graphene. This kind of nano material overcomes some shortcomings of traditional quantum dots. It not only has excellent optical performance and small size characteristics, but also has good biocompatibility, easy to realize surface functionalization, and is used in biochemical sensing, imaging analysis, and environmental detection. , Photocatalysis technology, metal ion detection and drug carriers...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 龙云飞刘豪敏袁敏莫平高健王星林
Owner HUNAN UNIV OF SCI & TECH
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