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Visual colorimetric detection method of organophosphorus pesticides

An organophosphorus pesticide and detection method technology, applied in the fields of nanotechnology and analysis and detection, can solve the problems of professional operation technology limitation, time-consuming and other problems, and achieve the advantages of overcoming the long detection time, simple operation, good social value and application prospect. Effect

Active Publication Date: 2019-12-31
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of traditional organophosphorus pesticide detection methods that are time-consuming and limited by professional operation techniques, combine the synthesis and performance advantages of gold nanorods, construct a visual colorimetric sensor, and develop it into an organophosphorus pesticide A new method for visual detection

Method used

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  • Visual colorimetric detection method of organophosphorus pesticides
  • Visual colorimetric detection method of organophosphorus pesticides
  • Visual colorimetric detection method of organophosphorus pesticides

Examples

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Effect test

Embodiment 1

[0032] Example 1 Feasibility verification of visual colorimetric detection method for organophosphorus pesticide detection

[0033] Prepare the solutions required for the construction of a visual colorimetric sensor: put the synthesized gold nanorod stock solution at room temperature for use, prepare 250 μg / mL acetylcholinesterase solution, prepare 100 mM ATCI solution, and prepare 0.41 mM KIO 3 solution, prepare glycine-hydrochloric acid buffer solution (pH 2.2), and place it in a refrigerator at 4°C until use.

[0034] The effect of each reaction component on the spectral signal was systematically investigated by the single variable method.

[0035] (1) Add 250 μL of glycine-hydrochloric acid buffer solution (pH 2.2) into an EP tube with a volume of 0.5 mL;

[0036] (2) Add 250 μL of gold nanorod stock solution;

[0037] (3) Add 8 μL of acetylcholinesterase solution (250 μg / mL), 20 μL of KIO 3 solution (0.41 mM) and 8 μL of ATCI solution (100 mM), mix gently, and place in...

Embodiment 2

[0041] Example 2 Transmission Electron Microscopy (TEM) Characterization of Organophosphorus Pesticide-regulated Gold Nanorod Etching

[0042] Using different concentrations of organophosphorus pesticide solutions, the effects of different concentrations of organophosphorus pesticides on gold nanorods were intuitively analyzed.

[0043] (1) Add 8 μL of acetylcholinesterase solution (250 μg / mL) and 10 μL of organophosphorus pesticide solution into a 0.5 mL EP tube, mix well, and incubate at room temperature for 25 min;

[0044] (2) Add 8 μL of ATCI solution (100 mM) to the mixed solution in (1), mix gently, and incubate in a water bath at 37 °C for 60 min;

[0045] (3) Add 250 μL of glycine-hydrochloric acid buffer solution (pH 2.2) into an EP tube with a volume of 0.5 mL;

[0046] (4) Add 250 μL of gold nanorod stock solution and 20 μL of KIO 3 solution (0.41 mM), gently mix;

[0047] (5) Add the mixture in (2) to (4) and mix gently, place in a water bath at 50°C for 15 minut...

Embodiment 3

[0049] Example 3 Optimization of Experimental Conditions for Visual Colorimetric Detection Method for Detection of Organophosphorus Pesticides

[0050] Through the single variable method, the influence of each reaction factor on the ultraviolet spectrum signal was determined respectively.

[0051] (1) ATCI concentration optimization: Take 250 μL of glycine-hydrochloric acid buffer solution (pH 2.2) and add it to an EP tube with a volume of 0.5 mL; then add 250 μL of gold nanorod stock solution and 20 μL of KIO 3 solution (0.14 mM), mix gently; finally add different volumes of ATCI solution (100 mM), mix gently, and place in a water bath at 50 °C for 15 minutes; directly measure and record the absorption of each group of solutions in the range of 400 nm-800 nm The peak position value was obtained to obtain the influence of different concentrations of ATCI on the formation of the visual colorimetric sensor and the ultraviolet spectrum, and the experiment was repeated three times...

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Abstract

Organophosphorus pesticides (OPs) are highly favored due to effects of high efficiency and wide action and are normally used as agricultural pesticides and herbicides, while a series of serious healthproblems, such as dizziness, vomiting, diarrhea and muscle tremor even death, can be caused to a human body due to OPs residue, and thus, a real-time quantitative detection method for OPs is urgentlyneeded to be designed and developed. Here, a visual colorimetric detection technology is built for the organophosphorus pesticides. According to the visual colorimetric detection technology, triazophos is selected as an organophosphorus pesticide representative model molecule, acetylthiocholine iodide (ATCI) is used as a acetylcholinesterase (AchE) catalytic substrate, the ATCI can be catalyzed and decomposed to sulfhydryl choline, and I2-induced gold nanorod etching can be prevented by reduction sulfhydryl at a tail end; when OPs exist, the activity of the AchE is suppressed, no reduction sulfhydryl is generated, I<-> can be oxidized by KIO3 to generate I2, etching reaction of the gold nanorod is finally triggered by the I2, the gold nanorod with OPs concentration dependence longitudinally absorbs peak location blue shift, and color change of a gold nanorod solution is caused. By the novel method, favorable performance actual application ability of organophosphorus pesticide detection can be verified, and the method has obvious creativity and application value.

Description

technical field [0001] The invention belongs to the field of nanotechnology and analysis and detection, and relates to the construction of a gold nanorod etching visual colorimetric sensor based on the regulation and control of acetylcholinesterase activity and a new method for the detection and analysis of organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are favored for their high efficiency and wide range of effects, and are often used as agricultural insecticides and herbicides. At present, due to the abuse of organophosphorus pesticides in traditional agricultural practices and modern industries, pesticide residues in air, water, soil, agricultural products, and food have increased. At the same time, organophosphorus residues will have a series of serious impacts on human health, so the detection of organophosphorus residue levels is very urgent, and it is very important to design and develop real-time quantitative detection methods ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31C12Q1/46
CPCG01N21/78G01N21/31C12Q1/46G01N2333/918
Inventor 卿志和杨荣华李友楠
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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