A kind of dual-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application

A dual emission fluorescence, molecular imprinting technology, applied in luminescent materials, nanotechnology, nanotechnology and other directions, can solve the problems of complex treatment, dopamine human harm, expensive chromatography instruments, etc., to improve selectivity and accurate detection results.

Active Publication Date: 2021-06-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The harm of abnormal dopamine (DA) content to the human body cannot be underestimated. At present, most of the methods for detecting dopamine are realized by chromatography, but the instruments used in chromatography are relatively expensive, and the pretreatment of sample detection is relatively complicated.

Method used

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  • A kind of dual-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application
  • A kind of dual-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application
  • A kind of dual-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application

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Effect test

Embodiment 1

[0035] CdTe QDs were prepared by a hydrothermal method. First prepare the precursor: prepare a clean centrifuge tube, mix 51 mg Te powder, 100 mg NaBH 4 and 1.5 mL of double-distilled water were added to the centrifuge tube in turn, and a needle was inserted into the cover of the centrifuge tube to discharge the excess hydrogen in the reaction, and then the centrifuge tube was placed in an ultrasonic machine for 1.0 h of ultrasonic reaction to obtain the precursor of CdTe QDs NaHTe. 0.3651 g CdCl 2 2.5H 2 O and 0.1777 mL of thioglycolic acid solution were made into a mixed solution, diluted with 100 mL of distilled water, and the pH of the mixed solution was adjusted to 11.2 with a NaOH solution with a concentration of 1 mol / L, nitrogen was introduced and stirred, and the precursor was mixed half an hour later NaHTe was quickly added to the mixed solution, and the reaction was refluxed in an oil bath at 140°C. Nitrogen gas was kept flowing slowly throughout the reaction, an...

Embodiment 2

[0039] First prepare the precursor: prepare a clean centrifuge tube, mix 40 mg Te powder, 80 mg NaBH 4 and 1.2 mL of double-distilled water were added to the centrifuge tube in turn, and a needle was inserted into the cover of the centrifuge tube to discharge the excess hydrogen in the reaction, and then the centrifuge tube was placed in an ultrasonic machine for 1.0 h of ultrasonic reaction to obtain the precursor of CdTe QDs NaHTe. 0.2 gCdCl 2 2.5H 2 O and 0.05mL mercaptoacetic acid solution were made into a mixed solution, diluted with 50 mL of distilled water, and the pH of the mixed solution was adjusted to 9.5 with a NaOH solution with a concentration of 1 mol / L, and argon gas was introduced and stirred, and the precursor was mixed half an hour later. Bulk NaHTe was quickly added to the mixed solution, and the reaction was refluxed in an oil bath at 140 ° C. The whole reaction was kept slowly flowing with argon gas. Red fluorescent CdTe QDs were obtained after 7 days o...

Embodiment 3

[0043] CdTe QDs were prepared by a hydrothermal method. First prepare the precursor: prepare a clean centrifuge tube, mix 60mg Te powder, 120 mg NaBH 4 and 1.8 mL of double-distilled water were added to the centrifuge tube in turn, and a needle was inserted into the cover of the centrifuge tube to discharge the excess hydrogen gas of the reaction, and then the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction for 1.0 h to obtain the precursor of CdTe QDs NaHTe. 0.5g CdCl 2 2.5H 2 O and 0.25mL thioglycolic acid solution were made into a mixed solution, added 500mL of distilled water to dilute, and the pH of the mixed solution was adjusted to 12 with a NaOH solution with a concentration of 1 mol / L, nitrogen gas was introduced and stirred, and after half an hour, the precursor NaHTe Quickly added to the mixed solution, reflux reaction in an oil bath at 140 °C, and nitrogen gas was kept flowing slowly throughout the reaction, and red fluorescent CdTe Q...

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Abstract

The invention relates to the technical field of preparation of biological functional materials, in particular to a double-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application. In the present invention, carbon quantum dots are coated in silica nanospheres as the inner core of a ratiometric fluorescent probe, and then red cadmium telluride quantum dots are used as response signals, and acrylamide and 4-vinylphenylboronic acid are used as bifunctional single Using N,N-methylenebisacrylamide as a cross-linking agent and dopamine as a template molecule in the alcohol phase through the initiation of azobisisobutyronitrile to synthesize a dual-emission fluorescence with a specific recognition site for dopamine Molecularly imprinted polymer nanoparticles, and then prepared a fluorescent detection test paper with visualization effect by impregnation method, so as to realize the visual detection of DA, and the detection results of DA in human serum samples proved that the fluorescence detection paper prepared by this method The detection test paper can be used for semi-quantitative detection of DA in actual complex samples.

Description

technical field [0001] The invention relates to the technical field of preparation of biological functional materials, in particular to a double-emission fluorescent molecularly imprinted polymer nanoparticle and its preparation method and application. Background technique [0002] Molecular imprinting technology (MIT) is a process of preparing polymers with specific recognition ability for a specific molecule, which can be used to prepare molecularly imprinted polymers (Molecularly imprinted polymers, MIPs) with specific selectivity. Fluorescence analysis has the advantages of fast, simple, sensitive, etc., and has great potential in the field of analysis. Compared with advanced instrument analysis such as high performance liquid chromatography, it has relatively large consumption in terms of solvent consumption, sample pretreatment, and test time. The advantages. [0003] More and more scientific researchers choose quantum dots as the emission source of fluorescent signal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/06C09K11/65C09K11/59C09K11/88B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00C09K11/025C09K11/06C09K11/592C09K11/65C09K11/883G01N21/6428G01N2021/6439
Inventor 王吉祥徐叶青潘国庆闫永胜
Owner JIANGSU UNIV
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