96results about How to "Eliminate cross-contamination" patented technology

Surgical articles that are conveniently and securely coupled are disclosed. Improved surgical procedures are also disclosed.

A method for forming through-wafer interconnects (TWI) in a substrate of a thickness in excess of that of a semiconductor die such as a semiconductor wafer. Blind holes are formed from the active surface, sidewalls thereof passivated and coated with a solder-wetting material. A vent hole is then formed from the opposite surface (e.g., wafer back side) to intersect the blind hole. The blind hole is solder filled, followed by back thinning of the vent hole portion of the wafer to a final substrate thickness to expose the solder and solder-wetting material at both the active surface and the thinned back side. A metal layer such as nickel, having a glass transition temperature greater than that of the solder, may be plated to form a dam structure covering one or both ends of the TWI including the solder and solder-wetting material to prevent leakage of molten solder from the TWI during high temperature excursions. Intermediate structures of semiconductor devices, semiconductor devices and systems are also disclosed.

The invention concerns reagent delivery system for an apparatus for processing of biological samples arranged on microscope slides, comprising a reagent section having one or more reagent containers; a slide section in which at least one microscope slide is arranged; a probe for dispensing a portion of reagent onto a predetermined microscope slide, and means for handling the probe. The probe comprises a continuous prove tubing element extending through a rigid probe member and connecting the probe tip to a pneumatic pressure regulation device. The reagent containers are adapted for cooperation with the probe tip. In this manner a high though-put and a very low carry over of fluid residues is achieved since there is no assembled parts making up the inside volume of the probe in which the fluid may be retained.

A device for supporting and moving a load within an area may include a motive construct configured to support the load and move the load to a ground location within the area; a lift mechanism coupled to the motive construct, the lift mechanism being configured to effect movement of the motive construct to a height relative to the ground location; and a remote controller operatively coupled to the device via at least one of a cable connection and a wireless connection, the remote controller being configured to steer the motive construct and/or to control the lift mechanism. The device may further include at least one sensor to detect a characteristic of the load. The device may further include a braking system.

An apparatus for safely handling and securely holding a multitude of large and heavy open topped pails, for loading ingredients into these pails, and for stirring the ingredients within these pails into a fully homogenized state. The apparatus is adapted for simple removal and easy cleaning of the stirring components, and to minimize mess and eliminate cross contamination.

A microarray contact printing is formed from at least one capillary tube. The tip has concentric reservoir and printing capillary tubes, with a first capillary tube (24) and a second capillary tube (22) having an inner bore (26) with an inner diameter larger than an outer diameter of the first capillar tube (24) so that the second capillary tube (22) partially overlaps a proximal end of the first capillary tube (24). The first capillary tube (24) has a contact surface (36) at a distal end. The inner bore of the first capillary tube (24) is adapted for drawing the printing solution retained in the second capillary tube (22) and depositing a drop of a solution on a printing substrate when the contact surface (36) is moved proximate the substrate.

The invention belongs to the technical field of diagnostic reagents, and particularly provides a method for amplifying and genotyping nucleic acid genes of HPV (Human Papilloma Virus) and an assay kit for the same. The method comprises the following steps of: carrying out multiple real-time quantitative fluorescence gene amplifications (QPCR) by using a hybrid primer; quantitatively determining high-risk subtype HPVs by using a quantitative probe; and differentiating HPV 16 and 18 subtypes in types by using a typing probe. The hybrid primer is designed by conservative sequences at two sides of a special area of an E1 gene encoded by the HPV; and the genotyping probe is designed according to a type-specific sequence at the center of the area. Gradient dilutions of recombinant plasmids containing 16 and 18 subtype E1-area genes are taken as standard reference. The kit comprises a cervical brush, a sample storage tube/liquid, a primer, a probe, a Taq enzyme & reaction buffer solution, and a standard contrast reference. The system can be used for quantitatively detecting 13 high-risk subtype HPVs (HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV-68) and differentiating two subtypes HPV-16 and HPV-18 in types.

A multi-well manifold assembly and method for reducing cross-contamination in continuous synthesis reactions in channels of microfluidic devices, for example oligonucleotide synthesis.

A single use disposable thermometer/biotherm including devices and methods for preventing food cross-contamination and reducing foodborne illnesses. A single-use biotherm includes a biotherm body having a proximal end and a distal end, with a frangible portion fracturably attached to the biotherm body. A temperature sensitive arrangement is located in or on the biotherm body. A first temperature indicating arrangement is located in or on the frangible portion and coupled to the temperature sensitive arrangement. Fracturing the frangible portion from the biotherm body captures the temperature indicated by the temperature indicating arrangement. A method of reducing foodborne disease involves providing a single use biotherm; inserting the biotherm into a food product; and receiving an indication of the temperature of the food from the biotherm. The biotherm is then disposed after receiving the temperature indication from the food product.

The manufacture of cellular product such as a protein by expression from a cell line in a bioreactor is carried out wherein the bioreactor is a container made of flexible film, at least the interior surface of the container being fluoropolymer, and in addition, the nutrient medium and other agents used in the fermentation or cell culture process can be prepared in a separate container of the same film.

The invention discloses an intelligent hotel workflow management system, a process management method and a check-in registering method. The management system comprises a mobile terminal, a backstage server and a reception terminal, wherein the mobile terminal is respectively connected with the backstage server and the reception terminal, and the backstage server is connected with the reception terminal. The process management method comprises the steps of: logging in a system platform on a mobile terminal, judging a logged privilege role after verification and then carrying out corresponding operation. The check-in registering method comprises the process of: generating an order in the reception terminal, prearranging rooms, finishing check-in registering preparation, handling check-in procedures, serving customers during the process the customers are in the hotel, carrying out check-out procedures by the customers, clearing up check-in records of the customers and confirming the correctness of the check-in records with the customers. According to the management system, the working achievements of staff can be recorded objectively and accurately, the shortage of subjective judgment by supervisors is overcome, and the fairness of the management can be realized to the maximum extent.

The invention provides a reagent disk comprising a substrate, a first rotating shaft rotatablely arranged on the substrate, a reagent rack assembly used for placing a reagent bottle, and a driving element used for driving the first rotating shaft to rotate, wherein the reagent rack assembly comprises a connecting shaft connected with the first rotating shaft. According to the reagent disk provided by the invention, the driving element is used for providing motive power to drive the first rotating shaft and the reagent rack assembly to rotate so as to uniformly mix magnetic bead reagents while placing the reagents; since the uniformly mixed reagent is directly selected in the reagent bottle when needing to select the reagent to perform experimental analysis, the magnetic bead reagents liable to generate settlement are not specially mixed, thereby omitting the time of singly performing the uniform mixing operation, and further shortening the time sequence period of the whole system and improving the integral efficiency of a full-automatic instrument. Moreover, no object directly touches the reagent in the reagent bottle in a working process of the reagent disk, thereby reducing the cleaning liquid consumption and completely eliminating the cross contamination of different reagents.

The invention discloses a kit capable of carrying out nucleic acid analysis under a totally-enclosed condition. The kit comprises a box body provided with a sample inlet and a sealing cover, a flexible upper cover in sealed butt joint with the box body to form a sealed cavity, one or more probes, and one or more functional containers. The sample inlet is positioned outside the sealed cavity; the sampling ends of the one or more probes are arranged in the sealed cavity, and the peripheral sides of the probes are connected with the flexible upper cover in a sealed mode; and at least one functional container is communicated with the sample inlet; and corresponding functional reagents are contained in one or more containers. The invention also provides a device and a method for nucleic acid analysis by using the kit. The nucleic acid extraction probe, the analysis reagent, the corresponding functional container and the like are sealed in independent space, so that a problem of cross contamination in the processes of sample pretreatment, transfer, amplification reaction and the like can be effectively solved, and the kit is suitable for multiple application fields of disease diagnosis,water quality detection, air monitoring, gene analysis, microbial research, pathogen detection and the like.

The invention discloses a sulfur dioxide analyzer based on an ultra-violet light-emitting diode, comprising the ultra-violet light-emitting diode, an absorption cell, a spectrometer and a computer connected on the spectrometer, wherein the two sides of the absorption cell are respectively provided with an incident window and an emergent window which correspond to each other and are transparent to ultra-violet rays, and the absorption cell is also provided with an air inlet and an air outlet; an incident optical fiber is connected between the ultra-violet light-emitting diode and the incident window of the absorption cell, and the two ends of the incident optical fiber are respectively provide with a collimator; an emergent optical fiber is connected between the emergent window of the absorption cell and the spectrometer, and the two ends of the emergent optical fiber are respectively provided with a collimator; and the included angle between the incident window and the ultra-violet light path and the included angle between the emergent window and the transmitted ultra-violet light path are acute angles or obtuse angles close to right angles. The invention also discloses a sulfur dioxide analyzing method based on the ultra-violet light-emitting diode. The invention has the advantages of accurate measurement, large measurement range and short measurement time.

The invention provides homogeneous olaquindox granules and a preparation method thereof. The homogeneous olaquindox granules are prepared form, by weight, 2-60% of olaquindox raw materials and 40-98% of auxiliary materials; one or several of hydrogenated castor oil, hydrogenated soybean oil, stearic acid, glyceryl monostearate, solid lipid, animal wax, vegetable wax and solid-state polyethylene glycol is/are selected as the auxiliary materials. The preparation method comprises the steps that firstly, at 60-90 DEG C, the auxiliary materials are heated to be melted and stirred evenly, and then the olaquindox raw materials and the melted auxiliaries are mixed, sheared and evenly stirred to obtain mixed liquor; secondly, the mixed liquor is sprayed and condensed to be prepared into the granules, and then the homogeneous olaquindox granules are obtained. According to the homogeneous olaquindox granules and the preparation method thereof, main medicine in the homogeneous olaquindox granules is even in distribution and smooth in surface, and the fluidity and dispersity of the granules are improved; when the granules are used, the granules are easy to mix, and the problems that medicine is left and animals are poisoned due to the fact that olaquindox preparations are poor in homogeneity, the olaquindox preparations are stuck on the wall, and the olaquindox preparations are mixed unevenly when the olaquindox preparations are used.