Device and microchip for sorting particles

Abstract

Disclosed is a sorting device capable of high-speed analysis and sorting with safety, high speed, and low cost by preventing cross-contamination between samples, contamination of samples, biohazard to the users, obviating the needs for an expensive flow cell and an expensive orifice part and for fine adjustment of the flow cell and the orifice. The device (A) for sorting particles is provided with a microchip (1) in which a flow channel (11) through which liquid containing particles is flown and an orifice (12) for discharging the liquid flowing through the flow channel (11) to a space outside the chip are provided, a vibratory element (2) for making and ejecting a droplet of the liquid at the orifice (12), a charging means for giving charge to the ejected droplet (D), an optical detecting means (3) for detecting an optical characteristic of the particles flowing through the flow channel (11), paired electrodes (4,4) arranged along the direction of movement of the ejected droplet (D) and opposed with the moving droplet (D) therebetween, and two or more vessels for collecting the droplet (D) passing between the paired electrodes (4, 4).

Description

technical field [0001] The invention relates to a device and a microchip for sorting microparticles. More specifically, the present invention relates to a microparticle sorting apparatus or the like that detects, within a microchip, characteristics of microparticles flowing through a flow path formed in the chip, discharges liquid droplets containing the microparticles to the outside of the chip, and based on The characteristics of the detected microparticles control the direction of movement of the droplets for sorting. Background technique [0002] Traditionally, in order to identify the properties of microparticles such as biologically relevant microparticles (such as cells, microorganisms, liposomes) or synthetic particles (such as latex particles, gel particles, or industrial particles), devices have been utilized that combine the microparticles The dispersed liquid is introduced into the flow path, and the characteristics of the microparticles that have been introduce...

AI Technical Summary

Problems solved by technology

Therefore, as in a conventional flow cytometer (cell sorter), it is difficult to increase the fine particles and flow velocity and sorting rate, and it is difficult to measure cell count at high speed (for example, several thousand to tens of thousands of cells per second). characteristics and sorting

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