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Quick nucleic acid extracting device

An extraction device and nucleic acid technology, which is applied in the field of rapid nucleic acid extraction devices, can solve problems such as long diagnostic cycle, untimely diagnostic results, and easy misjudgment, and achieve the effects of avoiding pollution, eliminating cross-contamination, and improving purity

Active Publication Date: 2018-06-29
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The device is fast, convenient, suitable for on-site diagnosis, economical and practical nucleic acid extraction device, and is designed in one tube, does not need any other equipment when used, and can solve the problem of long diagnostic cycle and diagnostic Problems such as untimely results and easy misjudgment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, nucleic acid extraction device

[0053] Such as figure 1 and figure 2 As shown, the present invention provides a nucleic acid extraction device similar to a syringe structure, the nucleic acid extraction device includes a push rod 1, a hollow tube body 2 and a liquid storage chamber, and the push rod can be inserted into the tube body through the opening at the top of the tube body; A protruding structure 9 fixed at the bottom is arranged inside the tube.

[0054] In this embodiment, there are three liquid storage chambers inside the pipe body, which are the first liquid storage chamber 10, the second liquid storage chamber 12 and the third liquid storage chamber 14 from bottom to top; the liquid storage chambers are integrated with the push rod , so that the liquid storage chamber and the push rod can move up and down inside the tube body together. In other embodiments, the liquid storage chamber can also be fixedly connected with the push rod in oth...

Embodiment 2

[0073] The rapid extraction of embodiment 2, DNA

[0074] Use the nucleic acid extraction device described in Example 1 to quickly extract DNA, wherein the preset liquid is as shown in Table 1:

[0075] Table 1. Liquid components preset in each part of the device when extracting DNA

[0076] Cavity

20μL Proteinase K+200μL Buffer AL

first storage chamber

200 μL ethanol

Second reservoir

1.5mL 70% ethanol

third storage chamber

200 μL deionized distilled water

[0077] The effect of the above preset liquid is as follows

[0078] Chamber: lysate, lyses cells, releases nucleic acid substances, and provides a high-salt and weak-acid environment for the combination of nucleic acids and adsorption membranes. The first storage chamber: washing, washing away nucleic acid fragments and impurities of small molecules

[0079] The second liquid storage chamber: washing, washing away impurities such as protein and salt ions

[0080] The t...

Embodiment 3

[0092] The rapid extraction of embodiment 3, RNA

[0093] Use the nucleic acid extraction device described in Example 1 to quickly extract RNA, wherein the preset liquid is as shown in Table 2:

[0094] Table 2. Preset liquid components in each part of the device when extracting RNA

[0095] Cavity

6μL 2-Mercaptoethanol+594μL Buffer RLT

first storage chamber

600 μL ethanol

Second reservoir

1.5mL 70% ethanol

third storage chamber

200 μL RNAase-free deionized distilled water

[0096] Note: Buffer RLT added with 2-mercaptoethanol can be stored at room temperature for one month, and it is best to prepare the solution before use.

[0097] Such as Figure 6 As shown, the using method and working process of the nucleic acid extraction device are as follows:

[0098] 1) Unscrew the first sealing cap of the sampling device, and prepare animal tissue (about 25 mg, shredded as much as possible), or a blood sample that does not contain...

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Abstract

The invention discloses a quick nucleic acid extracting device. The quick nucleic acid extracting device comprises a pushing rod, a hollow tube body and a liquid storage chamber, wherein a bulged structure fixedly arranged on the bottom is arranged in the tube body; the liquid storage chamber is arranged inside the tube body; a liquid release mechanism is arranged on the bottom of the liquid storage chamber; the bulged structure is matched with the liquid release mechanism for releasing liquid inside the liquid storage chamber; and the lower part of the tube body is connected with a nucleic acid adsorption device; and the liquid storage chamber and the pushing rod are arranged, so that the liquid storage chamber and the pushing rod can move up and down inside the tube body.

Description

technical field [0001] The invention relates to nucleic acid extraction, in particular to a rapid nucleic acid extraction device. Background technique [0002] Generally speaking, nucleic acid extraction requires multi-step work. First, biological sample materials such as cells and tissue materials need to be crushed, nucleases are inactivated, nucleic acids are released, and other tissue or cell components such as proteins, polysaccharides, and lipids are removed. Thereby obtaining high quality nucleic acid. [0003] Since Swiss physician Friedrich Miescher first successfully extracted DNA from cells in 1869 to the 1990s, nucleic acid extraction has been a tedious, time-consuming task requiring the use of toxic reagents. Until recent years, with the emergence of solid-phase adsorption technology and the development of biochemistry, this problem has been greatly alleviated. However, the rapid extraction method of portable nucleic acid suitable for the field is still the bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/6806
CPCC12N15/1006C12Q1/6806
Inventor 袁向芬吴绍强吕继洲王彩霞李晓琳
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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