31 results about "Chromobacterium" patented technology

Provided are bioactive compounds and metabolites derived from Chromobacterium species culture responsible for controlling pests, compositions containing these compounds, methods for obtaining these compounds and methods of using these compounds and compositions for controlling pests.

Chromobacterium suttsuga sp. nov., a new species of the genus Chromobacterium which possesses insecticidal activity, is described. The invention also relates to insecticidally-active metabolites obtained from the strain and to insecticidal compositions comprising cultures of the strain and / or supernatants, filtrates, and extracts obtained from the strain, and use thereof to control insect pests.

Chromobacterium suttsuga sp. nov., a new species of the genus Chromobacterium which possesses insecticidal activity, is described. The invention also relates to insecticidally-active metabolites obtained from the strain and to insecticidal compositions comprising cultures of the strain and / or supernatants, filtrates, and extracts obtained from the strain, and use thereof to control insect pests.

The invention discloses a transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides. The transplantation and cultivation method comprises the steps of: exercising the tissue-culture bottle seedlings of the betula alnoides, preparing substrates, processing the substrates before transplantation, transplanting, and cultivating the seedlings of the betula alnoides in greenhouse seedling nurseries, wherein a seedling container is made of degradable non-woven fabrics, the transmittance of a shading net ranges from 40% to 50%, the seedling container is arranged ina seedling disc, holes are formed in the bottom surface of the seedling disc, the distance from the seedling disc to the ground is more than 15cm, the temperature is controlled between 20 to 26 DEG C, the relative air humidity ranges from 75% to 90%, the water content of the substrate ranges from 60% to 70%, and multiple-chromobacterium-prodigiosum wettable powder can be used for atomizing; afternursery-grown plants survive for 28 to 32 days, the transmittance of the shading net is controlled to 60% to 70%, and the relative air humidity is controlled to 60% to 70%; and after the nursery-grown plants survive for 50 to 60 days, the shading net is uncovered. According to the method disclosed by the invention, the bottle seedlings only need to be cultivated for 90 to 100 days to obtain finished seedlings, the survival rate is more than 91%, and the survival rate of the seedlings is improved by 1.94 times compared with that in the prior art.

The invention relates to oceanic streptomyces parvulus and application thereof on the aspect of quorum sensing inhibition. The oceanic streptomyces parvulus is HY026 and has the preservation number of CGMCC No.8493. The streptomyces parvulus disclosed by the invention has high-efficiency low-toxicity quorum sensing inhibitory activity and obvious antineoplastic activity; one active component of the oceanic streptomyces parvulus can have an obvious quorum sensing inhibition effect under the concentration of 50mug per piece, has an obvious inhibiting effect on generation of an indicator Chromobacterium.Violaceum 12472 under the concentration of 12.5mug / mL; and the oceanic streptomyces parvulus has an inhibition ratio of 64.9 percent, which is superior to those of a large number of quorum sensing inhibition active materials or crude extracts reported currently. The strain has high yield of active ingredients. The oceanic streptomyces parvulus is expected to be applied to the development of a novel antimicrobial substance which uses a quorum sensing system as a target point.

Polynucleotides encoding the polypeptides involved in biosynthesis of FK 228 and those involved in synthesis of a novel FK228 analog, thailandepsin are disclosed herein. Also provided are methods of making FK228, thailandepsin and analogs of these molecules and methods of using these FK228 analogs. Chromobacterium and Burkholderia gene inactivation mutants are provided. Methods of forming a disulfide bond in a chemical are also disclosed.

The invention relates to a bacterial population sensing inhibitor and a screening method thereof. The screening method is characterized by comprising the following steps of: drying algae and smashing; adding an ethanol solution for extracting; and detecting an extracting solution with bacterium biosensor chromobacterium CV026 and caproyl homoserine lactone, wherein the extracting solution is the bacterial population sensing inhibitor if a detection result is positive. The inhibitor screening method is quick and effective, and the bacterial population sensing inhibitor can be efficiently screened from marine algae. The bacterial population sensing inhibitor screened with the method can be applied to preservation of fresh foods or products, and is natural and nontoxic and has a better preservation effect in comparison to the conventional chemical synthesis inhibitor. The screening method is suitable for industrial production. The yield of the prepared inhibitor can well meet practical application.

The invention designs compound separated and extracted by marine fusarium (Fusarium sp. QY010) and application thereof. The compound is the active compound which is obtained by fermenting, separating and purifying the fusarium QY010, wherein the chemical name of the active compound is 5-butyl pyridine formic acid, and the active compound is obtained by screening the pseudomonas aeruginosa screening model QSIS2 and the chromobacterium type bacterial strain CV026. Within the concentration range that the pathogenic bacteria can not be killed, the compound has the double-inhibitory action to the quorum-sensing system of the pseudomonas aeruginosa and the chromobacterium, can restrain the pathogenic behavior of the bacterial quorum sensing regulation, can obviously reduce the yield of the chromobacterium and the mycetin, can reduce the virulence factors of the quorum-sensing regulation of the pseudomonas aeruginosa, and can weaken the drug resistance of the pathogenic bacteria. The invention has the advantages of being easy to obtain the preparation raw materials, easy to ferment and cultivate the bacterial strain, high in yield, and simple and easy to control the technical process for preparing the compound. Furthermore, the analogues derived by the compound can be used for the research and the development of novel antibacterial agents.

Polynucleotides encoding the polypeptides involved in biosynthesis of FK 228 and those involved in synthesis of a novel FK228 analog, thailandepsin are disclosed herein. Also provided are methods of making FK228, thailandepsin and analogs of these molecules and methods of using these FK228 analogs. Chromobacterium and Burkholderia gene inactivation mutants are provided. Methods of forming a disulfide bond in a chemical are also disclosed.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a novel microbial agent and a soybean planting method. The novel microbial agent disclosed by the invention comprises the following strains: 1) one or more of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, Herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthomonas, flavobacterium and wautersia; and 2) azospirillum and azotobacter. The novel microbial agent can improve the emergence rate and the survival rate of soybeans, improve the growth of soybean seedlings, promote the accumulation of organic matter, improve the disease resistance of the soybeans to harmful bacteria and improve the nitrogen fertilizer utilization rate of the soybeans.

The invention relates to oceanic streptomyces parvulus and application thereof on the aspect of quorum sensing inhibition. The oceanic streptomyces parvulus is HY026 and has the preservation number of CGMCC No.8493. The streptomyces parvulus disclosed by the invention has high-efficiency low-toxicity quorum sensing inhibitory activity and obvious antineoplastic activity; one active component of the oceanic streptomyces parvulus can have an obvious quorum sensing inhibition effect under the concentration of 50mug per piece, has an obvious inhibiting effect on generation of an indicator Chromobacterium.Violaceum 12472 under the concentration of 12.5mug / mL; and the oceanic streptomyces parvulus has an inhibition ratio of 64.9 percent, which is superior to those of a large number of quorum sensing inhibition active materials or crude extracts reported currently. The strain has high yield of active ingredients. The oceanic streptomyces parvulus is expected to be applied to the development of a novel antimicrobial substance which uses a quorum sensing system as a target point.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a microbial agent for promoting growth of oilseed rape and an oilseed rape planting method thereof. The microbial agent provided by the invention comprises the following bacteria: a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; B) acetobacter, and c) azospirillum. The microbial agent provided by the invention improves the yield of oilseed rape, and is matched with a specific oilseed rape planting method to increase the yield of oilseed rape per mu, reduce the nitrogen fertilizer demand, reduce harmful germs, restore the number of microorganisms in soil and increase the diversity of the microorganisms.

Strains of Chromobacterium sphagni sp. nov. are described which have insecticidal activity against insect larvae, in general, and lepidopteran insect larvae, in particular. A biocontrol agent containing one or more C. sphagni, media in which the C. sphagni, or both, and optionally a carrier are also described. Methods of killing insect larvae and methods of reducing insect populations in an area by applying to the area or an object an effective amount of the biocontrol agent are also described.

The present invention relates to the fields of malaria and dengue virus. More specifically, the present invention provides compositions and methods useful for the treatment and prevention of malaria and dengue virus. In particular embodiments, a composition comprises mosquito nectar feed and Chromobacterium sp_Panamam (Csp_P).

The invention discloses a sarafloxacin hydrochloride bactericide for silkworms and a preparation method for capsules thereof. The bactericide consists of sarafloxacin hydrochloride, sucrose powder and sodium carbonate in a weight ratio of 1: (0.5-1.5): (1.5-2.5). The method comprises the following steps of: mixing the materials uniformly, preparing a soft material by using 50 to 90 weight percent ethanol solution as mucilage, granulating, drying, reshaping, mixing uniformly, filling into No.0 capsules, making plates by using aluminum plastic, and packing, wherein each capsule contains 0.1 gram of sarafloxacin hydrochloride. The results show that the water solubility, the quality stability and the controllability of the researched sarafloxacin hydrochloride capsules for the silkworms are obviously enhanced, and the capsules have an obvious prevention and control effect on bacterial silkworm septicemia caused by infection of bacillus such as bacillus thuringiensis or chromobacterium prodigiosum.

The invention relates to the technical field of microorganisms, and particularly discloses a microbial agent and a planting method for promoting sesamum indicum growth. The microbial agent comprises the following bacteria of a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidothiobacter, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; and b) Frankia, and c) xanthobacter. The microbial agent provided by the invention can improve the vitality of sesamum indicum root systems, increase the mass production of sesamum indicum and improve the disease resistance of sesamum indicum; and the planting method can improve the planting density of the sesamum indicum and reduce the dependence of excessive fertilizer application.

A novel Chromobacterium phragmitis sp. nov. strain 113-1 (NRRL B-67133) is described, which has insecticidal activity against insect larvae, in general, and dipteran and lepidopteran insect larvae, in particular. A biocontrol agent containing Chromobacterium phragmitis sp. nov. strain 113-1 (NRRL B-67133) and optionally a carrier are also described. Methods of killing insect larvae and methods of reducing insect populations in an area by applying to the area or an object an effective amount of the biocontrol agent are also described.

The invention discloses a new bacterial strain—Achromobacter xylosoxidans AX and its application for degrading sulfide. The Achromobacter xylosoxidans AX is preserved in the China Type Culture Collection Center, address: China, Wuhan, Wuhan University, deposit number: CCTCC NO: M 2019483, date of deposit: June 24, 2019; the invention provides a Achromobacter xylosoxidans AX with sulfide degradation performance, and the strain can detoxify the initial The concentration is 100mg·L ‑1 Na 2 The S degradation rate reached 94.4%. The discovery of this degrading bacteria is of great significance for the efficient purification of sulfide in industrial wastewater.

Disclosed herein are the nucleotide sequences of the Chromobacterium subtsugae genes. In addition, the amino acid sequences of proteins encoded by the C. subtsugae genes are provided. Nucleic acids, vectors and polypeptides comprising the aforementioned sequences are also provided. Homologues, functional fragments and conservative variants of the aforementioned sequences are also provided. Compositions having pesticidal, bioremedial and plant growth-promoting activities comprising C. subtsugae genes and proteins, and methods for the use of these compositions, are also provided.

A novel Chromobacterium phragmitis sp. nov. strain 113-1 (NRRL B-67133) is described, which has insecticidal activity against insect larvae, in general, and dipteran and lepidopteran insect larvae, in particular. A biocontrol agent containing Chromobacterium phragmitis sp. nov. strain 113-1 (NRRL B-67133) and optionally a carrier are also described. Methods of killing insect larvae and methods of reducing insect populations in an area by applying to the area or an object an effective amount of the biocontrol agent are also described.

The invention discloses composition for controlling powdery mildew of astragalus and a preparation method of the composition. After monascus purpureus is cultured in a specific culture solution, partof thalli are subjected to enzymolysis, an enzymatic hydrolysate is obtained, the remaining thalli are further stimulated by the enzymatic hydrolysate and the specific culture solution, and an optimized monascus purpureus strain is obtained; the optimized monascus purpureus strain is fermented, traditional Chinese medicine extract and chromobacterium are added for further culture, and the composition with higher activity is obtained. The substance with higher activity is cultured by stimulating microbial metabolism, the preparation method of the composition is simple and controllable, no toxicsubstances are added, and the obtained composition does not contaminate soil and has great economic effect and social effect.

Stabilized biological pesticides comprising Chromobacterium species, filtrate, supernatant, extract or pesticidally active substance derived therefrom with pesticidal activity having improved shelf life due to maintenance of physical uniformity and longer insecticide activity after use due to higher resistance to degradation when exposed to sunlight are disclosed.

Strains of Chromobacterium sphagni sp. nov. are described which have insecticidal activity against insect larvae, in general, and lepidopteran insect larvae, in particular. A biocontrol agent containing one or more C. sphagni, media in which the C. sphagni, or both, and optionally a carrier are also described. Methods of killing insect larvae and methods of reducing insect populations in an area by applying to the area or an object an effective amount of the biocontrol agent are also described.

The invention discloses a transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides. The transplantation and cultivation method comprises the steps of: exercising the tissue-culture bottle seedlings of the betula alnoides, preparing substrates, processing the substrates before transplantation, transplanting, and cultivating the seedlings of the betula alnoides in greenhouse seedling nurseries, wherein a seedling container is made of degradable non-woven fabrics, the transmittance of a shading net ranges from 40% to 50%, the seedling container is arranged in a seedling disc, holes are formed in the bottom surface of the seedling disc, the distance from the seedling disc to the ground is more than 15cm, the temperature is controlled between 20 to 26 DEG C, the relative air humidity ranges from 75% to 90%, the water content of the substrate ranges from 60% to 70%, and multiple-chromobacterium-prodigiosum wettable powder can be used for atomizing; after nursery-grown plants survive for 28 to 32 days, the transmittance of the shading net is controlled to 60% to 70%, and the relative air humidity is controlled to 60% to 70%; and after the nursery-grown plants survive for 50 to 60 days, the shading net is uncovered. According to the method disclosed by the invention, the bottle seedlings only need to be cultivated for 90 to 100 days to obtain finished seedlings, the survival rate is more than 91%, and the survival rate of the seedlings is improved by 1.94 times compared with that in the prior art.

The present invention relates to the fields of malaria and dengue virus. More specifically, the present invention provides compositions and methods useful for the treatment and prevention of malaria and dengue virus. In particular embodiments, a composition comprises mosquito nectar feed and Chromobacterium sp_Panamam (Csp_P).

Disclosed herein are the nucleotide sequences of the Chromobacterium subtsugae genes. In addition, the amino acid sequences of proteins encoded by the C. subtsugae genes are provided. Nucleic acids, vectors and polypeptides comprising the aforementioned sequences are also provided. Homologues, functional fragments and conservative variants of the aforementioned sequences are also provided. Compositions having pesticidal, bioremedial and plant growth-promoting activities comprising C. subtsugae genes and proteins, and methods for the use of these compositions, are also provided.

Provided is a method for modulating soybean cyst nematode infestation of a plant comprising applying to the plant an effective amount of a composition comprising a Chromobacterium, Burkholderia, and / or Flavobacterium strain to modulate the soybean cyst nematode infestation. The composition may be applied directly to the seed or in a substrate for growing the plant, such as in the soil.

The present invention relates to a strain of Achromobacter sp. resistant to nickel ion toxicity and its application. The present invention provides a strain of Achromobacter sp. with nickel ion tolerance, whose deposit number is CGMCC No.2964. When the nickel ion concentration in the sewage is in the range of 0-10mg / L, the strain can use nitrate nitrogen as the only nitrogen source. Under aerobic conditions, the removal rate of nitrate nitrogen can reach 100%, and the removal rate of total nitrogen is also up to 100%. The invention has the advantages of convenient operation and high denitrification efficiency, and is of great significance for optimizing the sewage biological treatment system and reducing the influence of nickel ions on the sewage denitrification system.