Chromobacterium formulations, compostions, metabolites and their uses

A technology of chromobacteria and metabolites, applied in the formulation, components, metabolites and application fields of chromobacter, can solve problems such as harm to human health and difficulty in eradicating Salmonella

Active Publication Date: 2014-08-20
MARRONE BIO INNOVATIONS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The problem is poultry infested with pathogenic microorganisms such as Salmonella, a hazard to human health. These beetles live in garbage, wood, expanded plastic, fiberglass and polystyrene insulation panels in chicken houses. The

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  • Chromobacterium formulations, compostions, metabolites and their uses
  • Chromobacterium formulations, compostions, metabolites and their uses
  • Chromobacterium formulations, compostions, metabolites and their uses

Examples

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example

[0141] Above-mentioned composition is not limited in the following application examples, these examples only provide different concrete examples, but not limited to the related material, condition, weight ratio, process parameter and similar content etc. that the patent request of the present invention enumerates.

example 1

[0142] Example 1: Extraction of Cromard, Deoxyviorin and Violacein from Chromobacterium Subisga

[0143] The following steps were used to purify the compound from the culture broth of Bacillus albicans Subis:

[0144] The culture broth was taken from a 10-L fermentation broth of Chromobacterium subisga, passed through Amberlite XAD-7 resin only (Asolkar et al., 2006) at a rotation speed of 225 rpm The suspension was continuously shaken at room temperature for 2 hours for extraction, the resin and cell mass were filtered with cotton cloth, and then washed with distilled water to remove the salt. The resin, cell mass and cotton cloth were immersed in acetone / methanol (50 / 50) for 2 hours, then the acetone / methanol solution was filtered off, dried in vacuum using a rotary evaporator and the crude extract was precipitated. Then use Sephadex (Sephadex) LH20 size exclusion chromatography (CH 2 Cl 2 / CH 3 0H; 50 / 50) for fractionation, and separate out 7 fractions ( figure 1 ), th...

example 2

[0160] Example 2: Amino Acid Analysis of Cromard A

[0161] Cromard A (0.05 mg) was hydrolyzed by liquid phase hydrolysis (6N HCL, 1% phenol, 110 °C, vacuum treatment for 24 h), after cooling, the reaction mixture was dried and the hydrolyzate was diluted in buffer for With a volume of 1.0 mL, 50 μl of the sample solution was placed on the ion exchange column for analysis.

[0162] In order to be able to carry out standardized calibration, put the amino acid standard solution on the Na-based analyzer of Hitachi 8800 (Sigma (Sigma), A-9906) to analyze the protein hydrolyzate for determining the response factor, thereby performing the Hitachi 8800 analysis The instrument is calibrated for all amino acids. Each injection contained norleucine as an internal standard to allow correction of results for sample volume and chromatographic variables. The system employs Pickering sodium buffer, Pierce Sequana 1 grade hydrochloric acid (hydrolyzed), a Transgenomic ion exchange column, a...

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Abstract

Stabilized biological pesticides comprising Chromobacterium species, filtrate, supernatant, extract or pesticidally active substance derived therefrom with pesticidal activity having improved shelf life due to maintenance of physical uniformity and longer insecticide activity after use due to higher resistance to degradation when exposed to sunlight are disclosed.

Description

technical field [0001] The present invention is Chromobacterium (Chromobacterium) filtrate, supernatant and extract purposes, ingredients or formulations, originally used as acaricides and insecticides, compounds with insecticidal activity or metabolites thereof, especially for use in It is used to prevent and control one or more of the following pests, such as Acaridae, Scarabidae, Drosophila, Psyllididae, Apiidae, Muscidae, Anthropidae and Pseudomidae, etc. The formulation of the present invention further includes biological insecticides (also referred to as biological insecticides), especially in the filtrate, supernatant, extract, metabolite or derivatives of Chromobacterium-containing bacteria, which have insecticidal activity Compounds and methods for their manufacture and methods for controlling pest invasion. More specifically, the present invention provides biopesticide properties with high stability, longer shelf life due to its uniform physical properties, and more...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N43/36A01N33/02C12N1/20A01N63/20
CPCA01N43/90A01N63/02C12N1/20A01N63/20A01N25/00
Inventor 瑞那卡·阿索卡詹姆斯·纳木纳斯帕梅拉·麦伦
Owner MARRONE BIO INNOVATIONS
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