Compound separated and extracted by marine fusarium and application thereof
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A technology of Fusarium fungi and compounds, applied in the field of microbial pharmacy, can solve problems such as reduced pathogenic ability
Inactive Publication Date: 2011-03-02
OCEAN UNIV OF CHINA
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A large number of literatures show that the pathogenicity of strains missing the quorum sensing system is greatly reduced
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Embodiment 1
[0017] Example 1: Preparation of monomer compound formula I by fermenting marine Fusarium sp.QY010
[0018] 1. Fermentation culture uses the following medium: sorbitol 20g, maltose 20g, glutamine 10g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, tryptophan 0.5g, yeast extract 3g, dilute to 1000mL with aged sea water, adjust pH to 6.5, 121°C, 1.05kg / cm 2 Sterilize for 20 minutes.
[0019] 2. Fermentation process: After activating the marine Fusarium sp.QY010 plate, pick an appropriate amount of mycelium and inoculate it into a Erlenmeyer flask containing 150mL of the above-mentioned liquid medium, and culture it with shaking at 28°C for 10 days.
[0020] 3. Fermentation product preparation: After the fermentation, the fermented mycelium and the supernatant mixture were ultrasonically crushed, an equal volume of ethyl acetate was added, stirred overnight at room temperature, the supernatant was obtained by centrifugation at 6000rpm, and an equal volume of ethyl acetate was added to ...
Embodiment 2
[0028] Embodiment 2: compound formula I active test
[0029] 1. Quorum sensing inhibitory activity test of formula I
[0030] Screening model QSIS2 plate screening method: Cool 13.5mL of melted solid LB medium to 40°C, add 1.5mL of 60% sucrose, 150μL of overnight cultured QSIS2 bacterial solution, 6μL of 500μmol / L signal molecule 3-oxododecanoyl Homoserine lactone 3-oxo-C12-HSL, 75 μL of 5% (w / v) live bacteria dye red tetrazolium TTC and 24 μL of 50 mg / mL gentamicin were mixed and poured onto the plate. After the plate is solidified, punch holes with a puncher, add 3 μL of compounds of different concentrations to each hole, and then incubate in a 37°C incubator to observe the growth of bacterial circles around the sample holes. If the compound has quorum sensing inhibitory activity, then There should be a bacterial growth circle around the sample hole. The larger the diameter of the bacterial circle, the stronger the activity. The test results are shown in Figure 1a: compared...
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Abstract
The invention designs compound separated and extracted by marine fusarium (Fusarium sp. QY010) and application thereof. The compound is the active compound which is obtained by fermenting, separating and purifying the fusarium QY010, wherein the chemical name of the active compound is 5-butyl pyridine formic acid, and the active compound is obtained by screening the pseudomonas aeruginosa screening model QSIS2 and the chromobacterium type bacterial strain CV026. Within the concentration range that the pathogenic bacteria can not be killed, the compound has the double-inhibitory action to the quorum-sensing system of the pseudomonas aeruginosa and the chromobacterium, can restrain the pathogenic behavior of the bacterial quorum sensing regulation, can obviously reduce the yield of the chromobacterium and the mycetin, can reduce the virulence factors of the quorum-sensing regulation of the pseudomonas aeruginosa, and can weaken the drug resistance of the pathogenic bacteria. The invention has the advantages of being easy to obtain the preparation raw materials, easy to ferment and cultivate the bacterial strain, high in yield, and simple and easy to control the technical process for preparing the compound. Furthermore, the analogues derived by the compound can be used for the research and the development of novel antibacterial agents.
Description
Technical field: [0001] The invention belongs to the field of microbial pharmacy, and specifically designs a compound with antibacterial quorum sensing activity isolated and extracted from marine Fusarium sp. QY010 and its application. Background technique: [0002] Bacterial infection has always been a threat to human health. Since the theory of bacterial origin of infectious diseases was confirmed in the late 19th century, the treatment of human anti-infectious diseases has a relatively clear goal. The emergence of antibiotics is a miracle in the history of medicine, especially penicillin, as the earliest antibacterial drug, successfully solved the clinical problem of Staphylococcus aureus infection, and the macrolides and aminoglycoside antibiotics that came out later made pneumonia, The death rate from tuberculosis has been reduced by 80%. But most antibiotics fight infection by interfering with important life processes of pathogenic bacteria to kill the microbes or inh...
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