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A marine Streptomyces parvum and its application in quorum sensing inhibition

A technology of Streptomyces microenum and quorum sensing, applied in the biological field, can solve the problem of no reported active compounds, and achieve the effect of high quorum sensing inhibitory activity

Inactive Publication Date: 2015-08-26
YANGZHOU UNIV
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Problems solved by technology

[0006] The Chinese patent application with the application number 201010295829.0 discloses a strain of Streptomyces in the ocean and its quorum sensing inhibitory activity, but no specific active compound is reported in the patent application

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  • A marine Streptomyces parvum and its application in quorum sensing inhibition
  • A marine Streptomyces parvum and its application in quorum sensing inhibition
  • A marine Streptomyces parvum and its application in quorum sensing inhibition

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Embodiment Construction

[0016] Chromobacterium.Violaceum 12472 (Proceedings of the National Academy of Science USA. 2003, 100(20): 11660-11665) involved in the examples was provided by the laboratory of Professor Qian Peiyuan of Hong Kong University of Science and Technology.

[0017] Sample collection: In October 2011, upper seawater and sediment samples were collected from Lianyungang waters and stored in sterile bottles.

[0018] Isolation and cultivation of strain HY026: Seawater samples were treated in a water bath at 55°C for 1 hour, and after gradient dilution, they were spread and inoculated into Gaoshi No. 1 solid medium, cultured at 30°C, and the colony growth was observed every day from the next day. Pick the newly grown actinomycetes and inoculate them on fresh Gaoshi No. 1 solid medium for purification. The isolated 46 strains were respectively inoculated into Gaoshi No. 1 liquid medium, and cultured at 30° C. with shaking at 140 rpm for 5 days. The bacteria were removed by centrifugati...

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Abstract

The invention relates to oceanic streptomyces parvulus and application thereof on the aspect of quorum sensing inhibition. The oceanic streptomyces parvulus is HY026 and has the preservation number of CGMCC No.8493. The streptomyces parvulus disclosed by the invention has high-efficiency low-toxicity quorum sensing inhibitory activity and obvious antineoplastic activity; one active component of the oceanic streptomyces parvulus can have an obvious quorum sensing inhibition effect under the concentration of 50mug per piece, has an obvious inhibiting effect on generation of an indicator Chromobacterium.Violaceum 12472 under the concentration of 12.5mug / mL; and the oceanic streptomyces parvulus has an inhibition ratio of 64.9 percent, which is superior to those of a large number of quorum sensing inhibition active materials or crude extracts reported currently. The strain has high yield of active ingredients. The oceanic streptomyces parvulus is expected to be applied to the development of a novel antimicrobial substance which uses a quorum sensing system as a target point.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of marine streptomyces micronum and its application in quorum sensing inhibition. Background technique [0002] Quorum-sensing (QS) was first proposed by Fuqua et al. in 1994. It is a group behavior that bacteria self-regulate according to their own cell density changes. [0003] Many bacteria can produce and release a signal molecule called Auto Inducer (AI). As the density of the bacteria increases, the signal molecule accumulates to a certain concentration and binds to the cytoplasmic receptor protein to regulate the bacteria. The expression of related genes enables bacteria to form a group behavior to effectively resist environmental pressure and attack hosts (Antimicrobial Agents Chemotherapy, 2000, 44: 640-646; Molecular Microbiology, 2004, 53: 755-769). In some opportunistic pathogenic bacteria (such as Pseudomonas aeruginosa, Enterobacter agglomerates, S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/06A61K35/74A61P35/04A61P31/04C12R1/465
Inventor 缪莉董昆明姜芸周惠茹翟有朋周晓见
Owner YANGZHOU UNIV
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