63 results about "Antimicrobial substance" patented technology

The invention discloses an application of an antibacterial peptide and an application of the antibacterial peptide in preparation of antibacterial drugs for treating carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the antibacterial peptide is characterized in that the sequence is w-r-r-w-k-r-w-w-r-r, and the sequence is shown in the description. All amino acids are D-type amino acids; the antibacterial peptide can be used as an antibacterial substance in wash supplies, food preservative additives, medical consumable antibacterial agents and cosmetics, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and good in in-vivo antibacterial activity. Particularly, the antibacterial peptide has broad-spectrum killing activity against carbapenem pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the cell survival rate of the antibacterial peptide within the range of medium and low concentration (2 mu g / mL-32 mu g / mL) reaches half or more, the toxicity is small, the toxicity is almost avoided, the operability is high, and the antibacterial peptide can be widely applied to the field of medical application. The cost is low.

The application discloses a plant lactobacillus and application thereof in improving stretchability of acid coagulated cheese. Lactiplantibacillus plantarum The plant lactobacillus is named as plant lactobacillus (Lactobacillus plantarum) B11, is preserved in China Center for Type Culture Collection, has a preservation number of CCTCC NO: M 2025778 and a preservation address of Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The strain provided by the application can be directionally fermented to produce viscous exocellular polysaccharide and antibacterial substances. The acid whey fermented by the strain is combined with phosphate and sodium caseinate quality improver, so that the stretchability of the acid coagulated cheese is improved, the product is inhibited from being mildewed in the airing and storage processes, and meanwhile, the product quality safety can be ensured. The problems of stretching difficulty, low product yield and easy mildewing in the airing and storage processes in the prior art are solved.

The invention provides a bactericidal anticorrosive composite coating and a preparation method thereof. The composite coating is obtained by reacting and curing raw materials including epoxy resin, an antibacterial substance based on a Schiff base structure and a curing agent, the antibacterial substance based on the Schiff base structure is characterized in that the antibacterial substance based on the Schiff base structure is obtained by carrying out a Schiff base reaction on components including polyethyleneimine and an aldehyde antibacterial agent. In some embodiments, the feedstock further includes a functional polymer PBFGn; the functional polymer PBFGn is obtained by carrying out free radical copolymerization reaction on components including butyl acrylate, hexafluorobutyl methacrylate and glycidyl methacrylate. According to the preparation method of the composite coating, efficient antibacterial components are stably integrated into a tough polymer network through a chemical method and automatically migrate to the surface, and long-acting and stable anti-corrosion and antibacterial effects and improved mechanical properties can be achieved.

The application provides a food preservation method using plant exosome loaded with natural antibacterial substances, relates to the field of food preservation and biological materials, and comprises the following steps: loading natural antibacterial substances and the plant exosome under preset loading conditions to obtain a plant exosome loaded with natural antibacterial substances; and applying the plant exosome loaded with natural antibacterial substances to the food preservation method, wherein the natural antibacterial substances are loaded into the plant exosome, the obtained composite is dispersed in a dispersion medium for food surface treatment to obtain a preservation preparation, and the preservation preparation is applied to the surface of food to be preserved to form a preservation layer, so that the natural antibacterial substances are stably loaded, uniformly distributed and continuously delivered on the surface of the food, the problems of volatilization, inactivation and short action time of the natural antibacterial substances during use are reduced, the food preservation effect is more stable, and the shelf life is prolonged.

One embodiment of the present specification provides a method for preparing a low-crystallinity calcium phosphate-based antibacterial bone graft material, comprising the steps of: (a) adding a calcium precursor and a phosphate precursor at one time to synthesize calcium phosphate; (b) putting the calcium phosphate into an antibacterial substance precursor solution to react; (c) a step of freeze-drying the product of step (b); (d) a step of mixing the product of step (c) with a shape control agent and performing press molding to prepare a molded body; and (e) a step in which the shape control agent of the molded body is removed in a solvent having a temperature equal to or higher than the boiling point and then dried.

The invention belongs to the technical field of biological medicines, and particularly relates to a dual-targeting broad-spectrum chimeric bacteriophage antibacterial pharmaceutical preparation as well as a preparation method and application thereof. The antibacterial drug preparation comprises a bioactive substance and a liposome for coating the bioactive substance, the bioactive substance is a targeting chimeric M13 bacteriophage coupled with an antibacterial substance, and the surface of the liposome is coupled with a targeting antibody. According to the invention, the liposome and bacteriophage dual-targeting design is adopted, specific targeting to bacterial focuses can be realized, and the antibacterial substance is loaded on the mild M13 bacteriophage, so that the dosage of the antibacterial substance is reduced, and meanwhile, uncontrollable release of endotoxin is avoided; and finally, the intracellular and extracellular bacteria at the in-vivo focus are thoroughly killed under the cooperation of the liposome with the targeting capability and the customizable chimeric bacteriophage.

This disclosure relates to antimicrobial compositions comprising at least one cationic antimicrobial substance and at least one acidic glycolipid, as well as the use of such compositions in product formulations, particularly anionic formulations such as personal care, home care, and other product formulations.

The invention discloses an implant with a honeycomb-shaped micro-nano topological structure on the surface and a preparation method and application thereof, and the implant with the honeycomb-shaped micro-nano topological structure on the surface is a honeycomb-shaped micro-nano topological structure layer prepared on the surface of a titanium substrate through a template method. According to the invention, a unique hydrogenated titanium dioxide honeycomb-shaped surface micro-nano topological structure is designed on the surface of the titanium-based implant through a template method and plasma hydrotreating on the basis of not introducing an antibacterial substance coating and complex chemical components; the special surface treatment not only has excellent 800-815nm near-infrared light response photocatalytic performance and can generate a large number of reactive oxygen species to quickly eliminate bacterial infection, but also can regulate polarization of macrophages due to the unique surface morphology, effectively inhibit inflammatory response and regulate and control bone immune microenvironment to promote osteogenesis, and has a good application prospect. And therefore, more than two invasive operations are not required to be carried out on the patient, and the postoperative healing effect is better.

The invention relates to a fungicide composition for preventing and treating peanut root rot as well as preparation and application of the fungicide composition. The invention aims to solve the technical problems of unstable field control effect, high chemical pesticide residue and high drug resistance risk of biological control in peanut root rot control. Bacillus velezensis YJ02 with good biocompatibility is compounded with azoxystrobin (a biological fungicide generates antibacterial substances and induces plant defensive enzymes, and a chemical fungicide is combined to rapidly inhibit respiration of pathogenic bacteria) to prevent and treat peanut root rot; the technical effects that the efficient synergistic control effect (for example, the control effect reaches up to 86.79%) is achieved, the application amount of chemical pesticides is reduced (for example, the dosage of azoxystrobin is only 9.82% of the conventional dosage), the drug resistance risk of pathogenic bacteria is effectively reduced, the morbidity and disease index are reduced, and environment friendliness is achieved are achieved, and high economic benefits and social benefits are achieved.

The invention discloses an antibacterial substance management information system based on artificial intelligence, and relates to the field of medical information, a plurality of historical antibacterial grade sequences sorted according to time are acquired from historical antibacterial treatment data, and a gradient sequence based on adjacent time step difference values is generated; the system calculates the continuous gradient length based on the sliding window, wherein the continuous gradient length reflects the continuous upgrading or degrading trend of the antibacterial grade; in a self-attention layer of the Transform network, a time step with a larger continuous gradient length is endowed with a higher attention weight, so that a key change fragment in the antibacterial grade sequence is highlighted, and the Transform network is trained based on a historical antibacterial grade sequence to obtain an antibacterial grade prediction model. According to the invention, intelligent prediction and management optimization of the antibacterial drug grade in the future can be realized by using the antibacterial grade change characteristics.

The invention discloses a preparation method of an enhanced warp-knitted lace fabric, and aims to solve the problems that fibers such as cotton fibers with relatively high water absorption capacity do not have a quick-drying characteristic, have poor antibacterial performance after long-term retention of water, still go mouldy and stink, and are low in skin-friendly property. According to the technical scheme, the method is characterized by comprising the following steps that S1, spandex filaments are stretched to a certain drafting length, then nylon filaments are evenly wound on the outer surfaces of the stretched spandex filaments according to a certain twist degree, and the nylon elastic covering yarn is obtained. The antibacterial acarus killing layer is made of cotton fibers with high water absorption, and treatment is conducted through composite alkali liquor, alkali liquor, a mercaptoacetic acid solution and a silver nitrate solution in sequence in the preparation process, so that antibacterial substances can be stably embedded into the fibers instead of being only attached to the surface layers of the fibers, and the antibacterial acarus killing effect is achieved. Furthermore, good antibacterial performance can be kept in the states of multiple times of water washing and long-term soaking.

The present application belongs to the technical field of surgical implant medical devices for foot and ankle, and discloses a preset angle ankle arthrodesis device with functions of promoting bone integration and resisting bacteria, which has a preset angle suitable for tibiodal joint, ranging from 3° to 7°. The device further comprises a three-dimensional porous bone implant body, a surface of the three-dimensional porous bone implant body in contact with host bone tissue is a porous titanium alloy, a plurality of convex anti-rotation ridges are arranged on the upper and lower end faces of the three-dimensional porous bone implant body, the three-dimensional porous bone implant body is fixed by occlusion with the host bone through the anti-rotation ridges, an in-situ growth film layer is grown on the surface of the three-dimensional porous bone implant body, and the in-situ growth film layer comprises functional substances and antibacterial substances. The present application effectively solves the technical problems of slow bone integration, high risk of infection, rotation instability and insufficient mechanical support in ankle arthrodesis, significantly improves the success rate of fusion and reduces postoperative complications.

The invention discloses a gelled cell as well as a preparation method and application thereof. Relates to the field of synthetic biology and biomedicine. The gelated cell is a cell structure without cell viability, and comprises: a carrier cell framework comprising a cell membrane which retains the membrane protein content, the type and the lipid order of an original carrier cell; the intracellular hydrogel is formed in situ in the carrier cell framework through supramolecular host-guest interaction or cross-linking reaction, and is fused with the inner surface of a cell membrane; the antibacterial substance is loaded in the intracellular hydrogel; wherein pores are distributed on the cell membrane, the intracellular hydrogel is communicated with the external environment through the pores, and the loaded antibacterial substances are exposed. The gelated cell provided by the invention can simultaneously realize targeted delivery, contact sterilization and endotoxin adsorption, and avoids the problem of secondary inflammation caused by release of endotoxin in traditional antibiotic therapy and the problem of membrane protein loss or disordered arrangement in a cell membrane coating technology.

The invention relates to the technical field of bamboo product processing, in particular to a production process of a container based on strip-shaped bamboo splits. In the raw materials of the container, the first bamboo layer, the second bamboo layer, the third bamboo layer and the fourth bamboo layer are all formed by connecting common bamboo splits in series through lines, the bamboo container can be manufactured through multiple specific bonding procedures, the bamboo container has the advantage of being simple and efficient in process, the cross bonding structure of the first bamboo layer and the second bamboo layer can greatly enhance the mechanical strength of the bottom plate, and the service life of the bottom plate is prolonged. By arranging the supporting blocks, on one hand, the bottom plate can be lifted, ventilation of the bottom of a bearing object is facilitated, the bearing object can be used for loading fruits and vegetables, antibacterial substances naturally contained in bamboo wood can be utilized, and the storage period of the fruits and the vegetables is prolonged; the side plate structure can adapt to different shapes of the container by utilizing the curling property of the bamboo layer; certain air permeability is provided, and storage of fruits and vegetables is facilitated.

The application discloses a broad-spectrum bacteriostatic Clostridium subsp. and a preparation method and application thereof, and belongs to the technical field of microorganisms. The strain is named as DMS028, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 38049. The strain has broad-spectrum bacteriostatic activity, can simultaneously inhibit gram-negative bacteria Aeromonas hydrophila in aquaculture, gram-positive bacteria Clostridium perfringens in livestock and poultry breeding, and plant diseases such as Exserohilum longipes and Fusarium oxysporum, and overcomes the defects of narrow bacteriostatic spectrum of existing microbial preparations. The preparation method adopts two-stage temperature anaerobic culture, and adds lysine methyl ester, leucine ethyl ester, palmitoyl glycine and lauroyl arginine ethyl ester as an antibacterial substance synergist and a stabilizer, and has the advantages of simple process, convenient operation, no need of complex equipment, easy scale production, efficient high-activity bacterial body and antibacterial product.

The application discloses a plasma modified polyurethane medical dressing and a preparation method and application thereof, and relates to the technical field of medical dressings. The polyurethane sponge is cleaned and dried, and then is treated by using a plasma technology in an oxygen atmosphere to obtain treated polyurethane sponge; then, recombinant collagen with a HIS tag is prepared into a recombinant collagen solution; then, metal ions with antibacterial effects are added into the solution; the treated polyurethane sponge in step S1 is contacted with the recombinant collagen solution; finally, the modified polyurethane medical dressing is immersed into a high-molecular solution with a low isoelectric point and a negative charge under a neutral condition, and the medical dressing is obtained after drying and washing after immersion. The preparation method avoids the use of chemical cross-linking agents, ingeniously uses the tag of the recombinant collagen, simplifies an antibacterial substance loading process, and enables the antibacterial agent not to be excessive, so that the antibacterial agent can be used for a long time at a wound and can be safely applied to the fields of negative pressure drainage and wound care.

The invention discloses a tissue culture and rapid propagation method for stephania erecta, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a healthy stephania erecta stock plant, carrying out root irrigation treatment by using an aqueous solution containing antibacterial substances, and shearing a tender rattan section on the stock plant as an explant; after the explants are disinfected, the explants are inoculated to an induction culture medium I, dark culture is conducted for 7 days firstly, then the explants are transferred to an induction culture medium II to be subjected to light culture, and bud germination is promoted; cutting off the germinated tender shoots, inoculating the tender shoots into a proliferation culture medium, and carrying out proliferation culture to obtain blocky cluster buds; dividing the blocky cluster buds into single buds, transferring the single buds into a rooting and seedling strengthening culture medium, and performing rooting culture to obtain tissue culture seedlings; and hardening and transplanting the tissue culture seedlings with good rooting. Through control and optimization of various steps and multiple parameters in the tissue culture process, successful construction of the tissue culture and rapid propagation system of stephania erecta with zero pollution rate, zero browning rate, high proliferation rate, high rooting rate and high survival rate is realized.

To obtain an antimicrobial fiber product having a substance having antimicrobial activity on the surface and to provide a method for producing the fiber product.SOLUTION: According to one embodiment of the present invention, there is provided an antimicrobial fiber product comprising a fiber containing a thermoplastic resin at least on the surface thereof, and particles carried on the surface side of the fiber and containing a nitride. Further, there is provided a method for producing an antimicrobial fiber product, which comprises a step of preparing a fiber containing a thermoplastic resin at least on the surface thereof and particles containing a nitride, and a step of bringing the particles in a heated state into contact with the fiber to support the particles on the surface of the fiber, thereby obtaining the antimicrobial fiber product.SELECTED DRAWING: Figure 1

The invention discloses a plasma modified polyurethane medical dressing and a preparation method and application thereof.The preparation method comprises the steps that polyurethane sponge is cleaned and then dried, then the polyurethane sponge is treated in the oxygen atmosphere through the plasma technology, the treated polyurethane sponge is obtained, recombinant collagen with an HIS tag is prepared into a recombinant collagen solution, and the plasma modified polyurethane medical dressing is obtained. The preparation method comprises the following steps: preparing a polyurethane sponge solution, adding metal ions with an antibacterial effect into the solution, contacting the polyurethane sponge treated in the step S1 with a recombinant collagen solution, finally immersing the modified polyurethane medical dressing into a polymer solution which is low in isoelectric point and has negative charges under a neutral condition, and drying and washing to obtain the medical dressing after the infiltration is completed. According to the preparation method, the use of a chemical cross-linking agent is avoided, meanwhile, the tag of the recombinant collagen is ingeniously utilized, the antibacterial substance loading process is simplified, and the antibacterial agent is not excessive, can be used at a wound for a long time, and can be safely applied to the fields of negative pressure drainage, wound surface nursing and the like.

The application provides a surgical auxiliary instrument material with bacteriostatic performance and a preparation method thereof, relates to the technical field of bacteriostatic medical instruments, and comprises the following steps: heating and mixing polyethylene, polycaprolactone, styrene-butadiene block copolymer and a bacteriostatic precursor to obtain a mixture of the surgical auxiliary instrument material with bacteriostatic performance, and the raw materials of the bacteriostatic precursor include flax lignan, 4-vinyl-2,6-dichlorophenol and imino methacrylate. In view of the problem of poor wear resistance of the bacteriostatic surgical auxiliary instrument, the bacteriostatic precursor with high polymer coating flax lignan is prepared, the compatibility of flax lignan and the remaining high molecular materials (polyethylene, polylactic acid, styrene-butadiene block copolymer) is improved, the interface bonding performance is improved, the synergistic effect between flax lignan and other materials is exerted, the antibacterial substance is no longer a heterogeneous phase particle in the bacteriostatic surgical instrument, and the continuity, wear resistance and oxidation resistance of the whole material are maintained.

The invention discloses a wound dressing with both strong bacterial adhesion and high liquid absorbency, and relates to the technical field of medical dressings, the wound dressing is prepared from composite yarn, the composite yarn is of a two-layer structure, the two-layer structure of the composite yarn comprises an inner hydrophilic layer and an outer hydrophobic layer, and the inner hydrophilic layer and the outer hydrophobic layer are arranged on the outer hydrophilic layer. The composite yarn is formed by wrapping inner-layer hydrophilic fibers with outer-layer hydrophobic fibers, and an outer-layer hydrophobic layer is made of hydrophobic fiber filaments. The invention has the advantages of higher safety, no addition of any antibacterial substance, no risk, easily available materials, batch production, direct purchase of the used materials, and simple process.

The invention relates to the technical field of hygienic products, in particular to a manufacturing process of a long-lasting antibacterial bamboo filament duster cloth. Bamboo filaments obtained by planing a specific part of a bamboo material are selected, an inner layer bamboo green part is selected in the aspect of material selection, the planed bamboo filaments are subjected to the processes of drying, soaking softening, drying, gluing, stacking, compressing and cutting under specific conditions to obtain bamboo filament blocks, then the multiple bamboo filament blocks are distributed in a rectangular array mode, and the cleaning cloth is obtained through yarn edge sealing and reinforcing. Compared with the existing rag, the rag with the structure has the following advantages: 1, the bamboo is environment-friendly and easy to degrade; 2, due to the specific selected materials, the material has good toughness and strength, the surface is not easy to wear and deform, and the material has high durability; 3, the bamboo wood contains natural antibacterial substances, oil stains are easy to remove, and bacterium breeding can be inhibited in a high-temperature and high-humidity environment, so that the bamboo wood can be kept clean and sanitary for a long time; and 4, the hardness is moderate, and the cleaning efficiency on stubborn stains such as rice scars is high.

The present invention relates to an antimicrobial substance and a method for producing the same. The present invention also relates to the use of the antimicrobial substance. The antimicrobial substance disclosed in the present invention is composed of a complex of an acid-form sophorolipid and a Group 11 element ion. The present disclosure provides the use of the antimicrobial substance as an antimicrobial agent and as a surfactant.

This invention relates to the field of automated microbial antimicrobial susceptibility testing technology, specifically to an automatic device for measuring the diameter of inhibition zones. The device includes an image acquisition module, an antimicrobial susceptibility test strip center positioning module, a radial transmittance profile construction module, a diffusion-MIC inversion module, and a diameter output module. This invention reconstructs the traditional inhibition zone measurement paradigm based on image edge detection and geometric circle fitting into a radial profile inversion paradigm based on a physical model of antimicrobial substance diffusion in agar. It constructs a radial transmittance profile and a family of azimuth profiles with the center of the antimicrobial susceptibility test strip as the polar coordinate origin, inversely infers the local diffusion coefficient field, and uses Fick's second law to nonlinearly invert the minimum inhibitory concentration (MIC) radius. Simultaneously, it separates dual-zone resistant subgroups through residual multi-peak detection and achieves dynamic prediction through temporal differential constraints. This invention achieves a measurement standard deviation better than 0.1 mm, supports dual-zone identification and culture endpoint prediction, and is significantly superior to existing automatic measurement schemes based on edge detection.

The antibacterial suture packaging kit of the present invention comprises: a first storage section 140 whose internal space is sealed by a joint 110 formed by fusing two synthetic resin sheets; a suture kit 130 housed in the first storage section 140; a second storage section 150 located adjacent to the first storage section 140, whose internal space is sealed by the joint 110; a liquid antibacterial substance 170 housed in the second storage section 150; and the first storage section 140 and the second storage section 150, respectively, to ensure a path for the movement of the antibacterial substance 170. The container includes a partition wall 300 that partially partitions the container, and an antimicrobial capsule 190 housed within the second housing section 150, which is filled with the antimicrobial substance 170 to the extent that it swells. The antimicrobial substance 170 is housed separately from the suture kit 130 by being filled within the antimicrobial capsule 190. When the second housing section 150 is pressed and the antimicrobial capsule 190 ruptures, the antimicrobial substance passes through the partition wall 300 and flows into the suture kit 130, where it is absorbed by the sutures 120.

The invention discloses a complex microbial inoculant for repairing alpine degraded grassland based on indigenous mycorrhizal fungi and preparation and use methods thereof, and relates to the technical field of environmental microbial ecological restoration, the complex microbial inoculant comprises: a carrier which is a mixture of highland barley straw powder and humic acid in a mass ratio of 3: 1; according to the arbuscular mycorrhizal fungi separated and screened from rhizosphere soil of alpine meadow degraded grassland in Qilian Mountain, the parasitic relationship between mycorrhiza and bacteria is utilized, aboriginal arbuscular mycorrhizal fungi hyphae provide a colonization space for phosphate-solubilizing bacteria, and the bacteria secrete organic acid to dissolve soil insoluble phosphorus and are directly transported to plant root systems through a hypha network, so that the phosphorus-solubilizing bacteria are degraded, and the phosphorus-solubilizing bacteria are degraded. According to the invention, a synergistic mechanism of'hypha-phosphorus channel 'is formed, meanwhile, bacillus subtilis is added to secrete antibacterial substances such as lipopeptide antibiotics and inhibiting pathogenic bacteria such as fusarium, functional microorganisms are enriched on the surface of hypha, and the functions of mutual excitation, ecological niche antagonism and the like of the functional microorganisms are utilized, so that the soil functionality and the stress resistance of host plants are enhanced.

The present application relates to the technical field of dust-free cloth, in particular to a high water absorption and antibacterial performance dust-free cloth. The present application overcomes the problem of poor water absorption and antibacterial performance of dust-free cloth. The present application realizes the removal of impurities on the surface of the fiber through the synergistic effect of ultrasonic cleaning of soap solution and esterase, and improves the water absorption performance of the fabric. The water absorption and antibacterial performance of the dust-free cloth are improved by activating the pretreated fabric and immersing it in the modified liquid. Through the lysozyme-chitosan composite antibacterial system, the limitation of single antibacterial mechanism is broken, and vacuum impregnation and freeze-drying are combined, so that the dust-free cloth can continuously release antibacterial substances during use, effectively inhibiting the growth of bacteria. Through the post-processing steps of plasma treatment and hot air circulation oven curing, the water absorption and antibacterial performance of the dust-free cloth are further optimized.

The invention relates to the technical field of agricultural microbial preparations, in particular to application of polyhydroxyalkanoate to improvement of phytopathogen antagonism of agricultural microorganisms. The invention finds that PHA can promote agricultural microorganisms to secrete antibacterial substances, can promote the colonization of the agricultural microorganisms in soil or plants, has the function of improving the antagonism of the agricultural microorganisms to phytopathogens, can improve the control effect of the agricultural microorganisms to the phytopathogens, and has a good application prospect. Good application prospects are realized in the fields of plant disease prevention and control and agricultural microbial preparation preparation.

The invention discloses a retrogen-loaded hydrogel water-absorbing antibacterial liner as well as a preparation method and application thereof, and the preparation method comprises the following steps: mixing a gelatin solution and a dialdehyde starch solution to obtain a Ge-Ds solution; the preparation method comprises the following steps: mixing a bacterial cellulose solution with a Ge-Ds solution to prepare a Ge-Ds-Bc solution; and mixing the Postbio YDFF-3 solution with the Ge-Ds-Bc solution, heating and stirring, cooling the mixture to room temperature, pouring into a culture dish, transferring to the temperature of-20 DEG C, preserving for 24 hours, and freeze-drying to obtain the hydrogel water-absorbing antibacterial liner loaded with the metabiotics. According to the invention, gelatin, dialdehyde starch and bacterial cellulose are used as matrix substances, and the postbiotic postbioYDFF-3 is added as an antibacterial substance to prepare the water-absorbing antibacterial liner for refrigeration and preservation of grass carp, the water-absorbing antibacterial liner is used for absorbing blood water or percolate of grass carp meat and reducing moisture required by microbial reproduction, and the antibacterial effect of the postbiotic postbioYDFF-3 is utilized to improve the refrigeration and preservation effects of grass carp. Therefore, growth of spoilage microorganisms in the grass carp meat is inhibited, and the refreshing time of the grass carp meat is prolonged.

The technology presented herein relates to a method of preparing a sample for analysis, the method comprising: a) providing a sample potentially containing an antimicrobial substance, such as an antibiotic; and b) treating the sample with an enzyme to inactivate the antimicrobial substance, where the analysis comprises determining the viability of the microorganism, and where the analysis comprises determining the immunocompetence against the microorganism. Also disclosed are methods including a sample microbial viability assay, a New Delhi metal-Beta-lactamase 1 (NDM-1) construct for the recombinant expression of New Delhi metal-Beta-lactamase 1 (NDM-1), a method for the recombinant production of New Delhi metal-Beta-lactamase 1 (NDM-1) protein, and a method for the recombinant production of New Delhi metal-Beta-lactamase 1 (NDM-1) protein. And the use of an antimicrobial substance inactivating enzyme for preparing a sample for analysis.