38 results about "Chromobacterium sp." patented technology

An E. coli host strain was engineered wherein genes adhE, IdhA, frdB, and pfIB were disrupted and novel butanol dehydrogenase gene, sadB, from Achromobacter xylosoxidans, was added to produce the isobutanol production host.

Provided are bioactive compounds and metabolites derived from Chromobacterium species culture responsible for controlling pests, compositions containing these compounds, methods for obtaining these compounds and methods of using these compounds and compositions for controlling pests.

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

The invention relates to a complex microbial agent for restoring coastline with heavy oil pollution, as well as a preparation method and applications thereof. The complex microbial agent comprises Sphingobacterium mizutaii, Achromobacter xylosoxidans and Achromobacter piechaudii, furthermore, the collection number of the Sphingobacterium mizutaii is CGMCC No.800; the collection number of the Achromobacter xylosoxidans is CGMCC No.8022; and the collection number of Achromobacter piechaudii is CGMCC No.8021. The complex microbial agent is good in salt resistance property, prominent in competitive advantages, and has a high-efficiency restoration effect on the crude oil pollution of coastline, especially refractory heavy oil contamination through the synergistic effect of the three bacteria, and can also be applied to restoration of saline alkali soil with overflow oil contamination.

Separation and extraction and structure identification of a novel quorum sensing inhibitor and an application thereof. The invention relates to a compound which is separated and extracted from rheinheimera aquimaris QSI02 and has a quorum sensing quenching activity and an application of the compound. In the invention, the rheinheimera aquimaris QSI02, which is sourced from marine sludge, is subjected to fermentation and cultivation and separation and purification to obtain a cyclodepeptide monomer compound which has a quorum sensing system inhibiting activity of chromobacterium violaceum and pseudomonas aeruginosa, wherein the chemical name of the compound is cyclo(L-tryptophan-L-serine). Compared with the action mechanism of a traditional anti-bacterial medicine, the action mechanism of active compound is that the compound acts on a novel anti-bacterial target point, wherein the compound only interferes in a pathogenic behavior regulated by bacteria quorum sensing without influences on growth of bacteria, so that no bacterial drug resistance is generated theoretically. The compound and derivatives thereof can be used for guiding development of a novel anti-bacterial drug as a guide compound in the fields of clinical researching, agricultural production and the like, so that the compound has important significance on human health and agriculture development.

The invention discloses a preparation method and application method of a thick oil viscosity reduction and degradation mixed microbial agent. The mixed microbial agent is prepared from an Acinetobacter sp. strain ZX-15, an Achromobacter pulmonis strain PI3-03, Acinetobacter sp.RS206 and a chrobactrum anthropi strain CON21. The acinetobacter sp.strain ZX-15 and the Achromobacter pulmonis strain PI3-03 in the preparation method are efficient petroleum hydrocarbon degradation bacteria, and the acinetobacter sp.RS206 and the chrobactrum anthropi strain CON21 are efficient heavy oil viscosity reduction bacteria. The thickened oil viscosity reduction and degradation mixed bacterial agent disclosed by the invention is a mixed bacterial agent constructed by artificial screening, is clear in straincomponent and simple in preparation process, has the high degradation efficiency on thickened oil, has a good viscosity reduction effect, and has good application prospects and industrial productionpotential in an environmental treatment method.

The invention discloses a compound fungicide for nitrogen removal by denitrification. The compound fungicide is prepared from the following bacteria by volume percent: 20-30% of paracoccus, 20-30% of ochrobactrum and 40-60% of achromobacter. The invention also discloses a preparation method for the compound fungicide for nitrogen removal by denitrification and an application thereof in treating sewage containing nitrate nitrogen. The compound fungicide for nitrogen removal by denitrification disclosed by the invention has excellent properties of high adaptability, short treating time, high degradation rate of nitrate nitrogen and high removal rate of COD under the synergistic effect of paracoccus, ochrobactrum and achromobacter; when the compound fungicide is applied to the sewage, the degradation rate of nitrate nitrogen and the removal rate of COD in the sewage are obviously increased; the cost is greatly saved; and the application prospect is wide.

The invention discloses achromobacter sp. and an application thereof to improvement of iron nutrition of peanuts. The achromobacter sp. is named as 1604IPR-02, and the preservation number is CGMCC NO.16955. The achromobacter sp. 1604IPR-02 is a gram-negative bacterium, does not have spores, has high siderophore production capacity, can convert ferrum which is slightly soluble in soil into effective ferrum, can increase soil nutrients, can promote absorption of the ferrum in the soil by plants, particularly can be successfully colonized in peanut rhizosphere soil, can promote growth of the peanuts, can increase yield, and has excellent application potential.

The invention discloses a microbial inhibitor. The microbial inhibitor provided in the invention is violacein substances (comprising violacein or violacein derivatives) which are extracted from Citrobacter freundii (pCOM10) strains, Citrobacter freundii (pCOMdeltaD) strains or Duganella B2 and have different chemical structures. Two of three extracts provided in the invention have strong inhibition on various plant causative fungi and gram positive bacteria. The extract from the Citrobacter freundii (pCOM10) strains can inhibit the various plant causative fungi, and the inhibition rate of the Citrobacter freundii (pCOM10) strains with the concentration of 4mg / mL on Phytophthora capsici can reach 100%. The microbial inhibitor establishes bases for developing agricultural antibiotics through utilizing violacein or the violacein derivatives.

The invention discloses a microbial remediation microbial agent and a preparation method thereof. The microbial remediation microbial agent comprises the following raw material: acinetobacter calcoaceticus; bacillus subtilis; bacillus licheniformis; pseudomonas aeruginosa; gordonia paraffinivorans; pseudochrobactrum saccharolyticum; microbacterium oxydans; and achromobacter denitrificans. The preparation method comprises the following steps: (1) weighing the strains according to the weight ratio shown in the specification; (2) strain activation; (3) primary seed fermentation; (4) secondary seed fermentation; (5) industrial fermentation; and (6) blending of the components to obtain a finished product of the microbial remediation liquid inoculant. The microbial remediation agent can degradetoxic and harmful long-chain hydrocarbon substances or organic macromolecular petroleum substances in the drilling cuttings into harmless substances such as low molecules, water and carbon dioxide which are acceptable to the environment, and the degradation rate reaches 90% or above.

The present invention discloses a strain of Achromobacter xylosoxidans with monomethylamine degradability and the application thereof. This strain, named Achromobacter xylosoxidans GDUTAN5, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University (No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province) with a deposit number of CCTCC NO: M 2017285. This Achromobacter xylosoxidans GDUTAN5 was Gram-negative and rod-shaped, and the colony appeared to be round, light yellow, opaque and smooth, having a diameter of 1-2 mm. The Achromobacter xylosoxidans GDUTAN5 of the present invention can be applied to environmental restoration, degrading monomethylamine in the environment at a high degradation efficiency. When it degraded monomethylamine for 96 h at a substrate concentration of 5 mg / L, the degradation efficiency could reach 92.3%.

The invention discloses an achromobacter. The achromobacter is classified and named as Achromobacter sp, has a strain name of MBZ-2, is preserved in the China General Microbiological Culture Collection Center on August 13, 2020, and has a preservation number of CGMCC No. 20529. The achromobacter can effectively degrade p-toluenesulfonic acid and a downstream product herbicide quizalofop-p-ethyl.

The invention discloses a strain of xanthobacter sp. T21 and application thereof. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on January 22, 2021, and has an accession number of GDMCC No: 61462. The Xanthobactersp. T21 disclosed by the invention is obtained through enrichment with TCEP as a unique carbon source, can efficiently degrade the TCEP, has relatively strong environmental adaptability, and presents degradation action on the TCEP under the conditions that a temperature range is 20-40 DEG C and a pH range is 4-9. Under a neutral and slightly alkaline condition, the degradation efficiency of the Xanthobacter sp. T21 on TCEP with a concentration of 50 mg / L can reach 80%-98% within 30 minutes, and the Xanthobacter sp. T21 has efficient degradation capacity on TCEP and has huge potential when applied to ecological restoration of organic phosphate pollution.

Disclosed herein is the nucleotide sequence of the Chromobacterium subtsugae genome. Also provided are the nucleotide sequences of open reading frames in the C. subtsugae genome (i.e., C. subtsugae genes). In addition, the amino acid sequences of proteins encoded by the C. subtsugae genome are provided. Nucleic acids, vectors and polypeptides comprising the aforementioned sequences are also provided. Homologues, functional fragments and conservative variants of the aforementioned sequences are also provided. Compositions having pesticidal, bioremedial and plant growth-promoting activities comprising C. subtsugae genes and proteins, and methods for the use of these compositions, are also provided.

The invention discloses an application of norharman in preparation of a quorum sensing inhibitor and a bacterial strain. Norharman exists in a marine bacterium 40DY182 secondary metabolite, so that generation of violacein and formation of a biofilm can be well inhibited; the generation of drug-resistant pseudomonas aeruginosa pyocyanine is reduced, the formation of the biofilm is inhibited, and when the concentration is increased, the inhibition effect on the activity of a bacterial quorum sensing system is enhanced.

Polyhydroxyalkanoate (PHA) synthase gene (SEQ ID NO:2) isolated from fresh water Chromobacterium sp. USM2, its amino acid sequence (SEQ ID NO: 1) and its use to produce polymers incorporating C3 to C7 monomer units, including poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) random copolymer P(3HB-co-3HHx), poly(3- hydroxybutyrate-co-3-hydroxycalerate) random copolymer P(3HB-co-3HV) and P(3HB-co-3HV-co-3HHx) terpolymer. Production of polymer by expression of SEQ ID NO:2 in recombinant hosts including microorganisms such as Cupriavidus or Pseudomonas.

The invention provides a preparation method and application of a camphor essential oil-based bacterial quorum sensing inhibitor. The preparation method comprises the following steps: preparing essential oil and preparing the bacterial quorum sensing inhibitor. By adopting the preparation method, the camphor essential oil is obtained from camphor leaves, and for the first time, experiments confirmthat the essential oil prepared with the preparation method provided by the invention has the activity of inhibiting a quorum sensing system of wild purple bacillus, so that the purple bacillus is notpathogenic, and the essential oil does not cause the survival pressure on the purple bacillus, so that the drug resistance is generated; the essential oil prepared with the preparation method provided by the invention can inhibit the activity of escherichia coli, the purple bacillus, staphylococcus aureus and pseudomonas aeruginosa, and is a novel antibacterial substance based on bacterial quorumsensing inhibition. The method has the advantages and characteristics that the raw material is easy to obtain, the preparation method of the novel bacterial quorum sensing inhibitor is simple and reliable, the bacteriostatic agent does not generate drug resistance to bacteria and has no toxic effects on the bacteria and the like.

The invention discloses a strain of achromobacter and its application in improving peanut iron nutrition. The achromobacter is named 1604IPR‑02, and the preservation number is CGMCC NO.16955. The Achromobacter 1604IPR-02 of the present invention is a gram-negative bacterium without spores, has a strong ability to produce siderophore, can convert insoluble iron in the soil into effective iron, can increase soil nutrients, and promote plant resistance to The absorption of soil iron, in particular, can successfully colonize the peanut rhizosphere soil, promote peanut growth and increase yield, and has great application potential.

The invention provides a separated gene for coding catechol 2,3-dioxygenase (C230). The C230 gene is derived from an achromobacter xylosoxidans LHB21 (A.xylosoxidans LHB21 for short) strain, has the overall length of 924bp, starts from an ATG initiation codon, ends with a TGA termination codon, and codes 307 amino acid residues; and the molecular weight of the expressed protein is 35.16kDa, and the enzyme activity of the expressed protein is 32U / mg.

The invention relates to the field of synthetic biology, in particular to gene deletion and its application, the gene deletion strain and its application in increasing the yield of microbial secondary metabolites. It is found through experiments that the deletion of genes YEL017C‑A, YEL017W and / or YER182W can increase the production of deoxyviobacterin precursor, deoxyviobacterin, violacein precursor and / or violacein.

The invention discloses a pesticide intermediate degrading strain. The degrading strain is Achromobacter denitrificans PH‑1, which was preserved in the China Ordinary Microorganism Strain Preservation Management Center on December 6, 2021. The strain preservation number is For: CGMCC NO.24038. The pesticide intermediate degrading bacterial strain PH-1 of the present invention can tolerate high concentration of sodium chloride, and is effective against organic pollutants 2-chloro-5-chloromethylpyridine and imidacloprid, fluazinam, and pyridine in pesticide wastewater. Downstream products such as fluazifop-methyl have high-efficiency degradation ability.

Disclosed herein are the nucleotide sequences of the Chromobacterium subtsugae genes. In addition, the amino acid sequences of proteins encoded by the C. subtsugae genes are provided. Nucleic acids, vectors and polypeptides comprising the aforementioned sequences are also provided. Homologues, functional fragments and conservative variants of the aforementioned sequences are also provided. Compositions having pesticidal, bioremedial and plant growth-promoting activities comprising C. subtsugae genes and proteins, and methods for the use of these compositions, are also provided.

The invention discloses a denitrification and denitrification composite bacterial agent, which is prepared from the following bacterial strains in volume percentage: 20-30% of Paracoccus, 20-30% of Paleobacterium pallidus, and 40-60% of Achromobacter. The invention also discloses the preparation method of the denitrification and denitrification composite bacterial agent and its application in the treatment of sewage containing nitrate nitrogen. The denitrification and denitrification compound bacterial agent of the present invention has the excellent performance of strong adaptability, short treatment time, high nitrate nitrogen degradation rate and COD removal rate through the synergistic effect of Paracoccus, Paleobacterium and Achromobacter, and is used in In sewage, the degradation rate of nitrate nitrogen and the removal rate of COD in sewage are significantly improved, the cost is greatly saved, and it has broad application prospects.

The invention provides a composition of a mixture containing a first phenylaminopyrimidinyl indole derivative and phenylaminopyrimidinyl indole derivative which are different from each other. The mixture can have synergistic antibacterial effects on streptococcus parasanguis, staphylococcus cohnii, neisseria gonorrhoeae, peptostreptococcus prevotii, staphylococcus hominis, serratia liquefaciens, achromobacter xylosoxidans, mycobacterium murinum, corynebacterium macginleyi, morganella morganii, staphylococcus capitis, streptococcus sanguis, streptococcus pyogenes, peptostreptococcus anaerobius,alkali cellulase producing chryseomonas luteola, aeromonas caviae, enterobacter cloacae, micrococcus luteus, dendritic microbacterium and legionella pneumophila with extracts of plants such as broad-leaved echinops roots, stonecrop, cyrtomium rhizomes, standish's lonicera and rhizoma alocasiae macrorrhizae.

The invention discloses an application of benzyl benzoate in preparation of bacterial colony induction activity inhibitors; in the method, an oceanobacillus sp. XC22919 fermented product is used for separation and extraction, and in a concentration range of 0-60 [mu]g / mL, bacterial growth of chromobacterium violaceum CV026 is not inhibited, but generation of chromobacterium violaceum purpurin canbe significantly decreased; and with gradual increase of the concentration, the effect of inhibiting generation of the purpurin is stronger; the effect of the compound in inhibiting colony induction is in a concentration dependence. In a concentration range of 0-100 [mu]g / mL, bacterial growth of pseudomonas aeruginosa PAO1 is not affected, but the expression of virulence factors of pseudomonas aeruginosa can be significantly reduced, the expression of pyocyanin, elastase, proteinase, biological body membranes and other virulence factors can be significantly reduced, and the effect of the compound in inhibiting colony induction is in the concentration dependence.

The invention belongs to the field of environmental pollution repairing and discloses a high-temperature-resistant aerobic denitrification bacterial strain, of which the classification name is Achromobacter sp. The bacterial strain has been preserved at China center for type culture collection at 2015 Jan.9 and has been assigned the accession number CCTCC NO: M2015025. The invention also discloses an application of the high-temperature-resistant aerobic denitrification bacteria. The strain grows well at 50 DEG C, has strong denitrification performance, is very less in accumulation of nitrite during denitrification and is free of secondary pollution. In the invention, the high-efficient aerobic denitrification bacteria are obtained through screening from a eutrophic lake water body, when being subjected to enlarged cultivation, the bacteria strain can be directly used for denitrification on lake water and can avoid environmental inadaptability of non-indigenous microorganisms. The bacteria strain has huge application potential and has a significant promoting effect on environmental protection for long term.

The invention discloses a strain of Xanthobacterium T21 and its application. The bacterium was deposited in the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on January 22, 2021, and the preservation number is GDMCC No: 61462. The Xanthobacter sp.T21 of the present invention is obtained by enriching TCEP as the only carbon source, can efficiently degrade TCEP, and has strong environmental adaptability, under the conditions of temperature range 20°C-40°C, pH range 4-9 Both of them showed the degradation of TCEP. Under neutral and slightly alkaline conditions, the degradation efficiency of 50mg / L TCEP can reach 80-98% within 30 minutes. The Xanthobacter sp.T21 of the present invention has an efficient degradation ability to TCEP, and is applied to the ecological restoration of organic phosphate pollution The potential is huge.

The present invention discloses a strain of Achromobacter sp, which is classified and named as Achromobacter sp, and the strain is named as MBZ-2, which has been preserved in the General Microbiology Center of the China Committee for the Preservation of Microorganisms with a preservation date of August 2020 On the 13th, the deposit number was CGMCC No.20529. The Achromobacter provided by the invention can effectively degrade p-toluenesulfonic acid and its downstream product herbicide quizalofop-p-ethyl.