44 results about "Achromobacter sp" patented technology

The invention relates to a microbial remediation method for petroleum-contaminated soil. The microbial remediation method is characterized in that pseudomonas aeruginosa, achromobacter sp and a biosurfactant crude product are combined to repair petroleum-contaminated soil or degrade petroleum in oil sludge. The method has remarkable degradation effect on petroleum-contaminated soil or petroleum hydrocarbon in the oil sludge. According to a test, initial oil content of the petroleum-contaminated soil is 20000 mg / kg to 130000 mg / kg, and degradation efficiency can reach 75-91%. The method has the advantages of being low in treatment cost, high in treatment efficiency, free of secondary pollution and the like.

The invention provides novel achromobacter sp. D-12 for efficiently degrading acetochlor of amide herbicides and application of the novel achromobacter sp. D-12. The strain was preserved in the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China on September, 27th, 2012, and the preservation number is CCTCC No: M2012386. The achromobacter sp. D-12 can be applied to the degradation of the acetochlor in a water body in a direct adding mode, and can be used for safely, efficiently and quickly degrading the acetochlor remained in the water; and a microbial inoculum containing the strain is easy to prepare, low in cost and convenient to use, and has a good application prospect.

The invention aims at developing a microbial method for processing gasoline petroleum hydrocarbons, and provides a microbial agent for degrading petroleum hydrocarbons and a using method of the microbial agent. The microbial agent is composed of Micrococcus sp., Pandoraea sp. and Achromobacter sp., preferably, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. in the microbial agent is (8-16): (5-12): (10-16), or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 4:4:5; or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 2:1:2. According to the using method of the microbial agent for degrading the petroleum hydrocarbons, the microbial agent is used for degrading gasoline petroleum hydrocarbons, wastewater containing the gasoline petroleum hydrocarbons, or soil containing the gasoline petroleum hydrocarbons. After being treated by the microbial agent, the gasoline petroleum hydrocarbons can be rapidly and completely degraded, and thus a new idea is provided for solving the environmental pollution problem caused by gasoline or other petroleum hydrocarbons.

The invention relates to a complex microbial agent for restoring coastline with heavy oil pollution, as well as a preparation method and applications thereof. The complex microbial agent comprises Sphingobacterium mizutaii, Achromobacter xylosoxidans and Achromobacter piechaudii, furthermore, the collection number of the Sphingobacterium mizutaii is CGMCC No.800; the collection number of the Achromobacter xylosoxidans is CGMCC No.8022; and the collection number of Achromobacter piechaudii is CGMCC No.8021. The complex microbial agent is good in salt resistance property, prominent in competitive advantages, and has a high-efficiency restoration effect on the crude oil pollution of coastline, especially refractory heavy oil contamination through the synergistic effect of the three bacteria, and can also be applied to restoration of saline alkali soil with overflow oil contamination.

The invention discloses a microbial leaching and chemical fixing joint repairing method for heavily polluted soil of a chromium slag yard. The method comprises the following steps: spraying soil or muck of a chromium slag yard with a composite bacteria liquid which is obtained by cultivating compound strains Pannonibacter phragmitetus BB and Achromobacter sp.Ch<-1>; restoring Cr(VI) in the obtained leacheate into Cr(III), forming sediments in an alkaline condition, precipitating and recycling chromium; and carrying out chemical fixing on the Cr(VI) which is not leached from the soil or muck through hydrazine hydrate. By virtue of the method, the soil and muck with three different Cr(VI) pollution levels are repaired; the total removal rates on chromium are respectively 11.4%, 30.2% and 14.0%; the removal rates on water-soluble Cr(VI) are respectively 99.6%, 99.5% and 98.3%; and the leaching toxicity concentration of the Cr(VI) in the soil or muck is lower than 0.5mg / L.

The invention relates to achromobacter sp. capable of tolerating the toxicity of sulfonamide antibiotics and application of the achromobacter sp. to sewage treatment. The achromobacter sp. capable of tolerating the toxicity of the sulfonamide antibiotics, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 2964. When a water body contains the sulfonamide antibiotics, the achromobacter sp. still can keep a complete cell structure and an efficient aerobic denitrification property. The strain can take nitrate nitrogen as an only nitrogen source, the removal rate of the nitrate nitrogen under the aerobic condition can reach 100 percent, and the removal rate of total nitrogen also can reach 90 percent to 100 percent. The achromobacter sp. provided by the invention has the advantages of simplicity in operation, high denitrification efficiency and the like, and has important meanings on optimization of a sewage biological treatment system and weakening of influences, caused by the sulfonamide antibiotics, on a sewage denitrification system.

The invention relates to a penicillin G acylase mutant constructed by genetic engineering. In comparison with wild type penicillin G acylase from achromobacter (Achromobacter sp.CCM 4824), the synthesis performance of the penicillin G acylase mutant is improved substantially, the maximum synthetic product / hydrolysate value S / H reaches 22.3 and is 3.9 times that of wild type enzyme, and various beta-lactam antibiotics can be efficiently synthesized catalytically. When the ratio of side chains to mother nucleus is 1.05:1, the conversion rate of the mother nucleus 6-APA, 7-ADCA, 7-ACCA and 7-APRA reaches 99.0% or above, and the penicillin G acylase mutant has wide industrial application prospects.

The invention provides an active fermentation broth and an application thereof, belonging to the technical field of biological pesticide. The active fermentation broth is obtained through the following steps: a, storing achromobacter sp. CD-253 with the preservation number of CGMCC No.6358 in an (LB) culture medium of yeast extract and tryptone (the yeast extract: 5.0g, the tryptone: 10.0g, sodium chloride: 10.0g, water: 1000ml, pH: 7.0); activating for 24 hours in the same liquid culture medium for use; b, inoculating 100ul of the activated strain into a triangular flask containing 50-250ml of the liquid LB culture medium, culturing for 96 hours under a condition with the temperature at 28-37 DEG C and the rotation speed at 120-180rpm to obtain culture broth, centrifuging the culture broth for 10 minutes at 8000rpm and then taking the supernatant to place in a refrigerator at 4 DEG C for later use, so that the active fermentation broth is obtained. The invention also provides the application of the active fermentation broth in preparing biological reagents for killing M. incognita, C. elegans and P. redivivus. The active matter provided by the invention has the advantages of low cost, convenient preparation, high efficiency and low toxicity.

The present invention discloses isolation of Penicillin Acylase (PA) from Achromobacter sp CCM 4824 expressed in recombinant strain E. coli BL21 CCM 7394 bearing the recombinant plasmid pKXIP1 and processing of PA into biocatalyst useful for the industrial synthesis of antibiotics. More particularly the invention discloses a synthesis of semi-synthetic [beta]-lactam antibiotics in the reaction mixture consisting of activated acyl-donor (D-p-hydroxyphenylglycine methyl ester or amide for Amoxicillin and Cefadroxil; D-phenylglycine methyl ester or amide for Ampicillin and Cephalexin) and nucleophile (6-APA or 7-ADCA) catalyzed by PA obtained from recombinant E. coli BL21 CCM 7394 as the biocatalyst.

The invention discloses achromobacter sp. and an application thereof to improvement of iron nutrition of peanuts. The achromobacter sp. is named as 1604IPR-02, and the preservation number is CGMCC NO.16955. The achromobacter sp. 1604IPR-02 is a gram-negative bacterium, does not have spores, has high siderophore production capacity, can convert ferrum which is slightly soluble in soil into effective ferrum, can increase soil nutrients, can promote absorption of the ferrum in the soil by plants, particularly can be successfully colonized in peanut rhizosphere soil, can promote growth of the peanuts, can increase yield, and has excellent application potential.

The invention relates to the technical field of biodegradation treatment, and aims at providing a preparation method and an application of a grease degrading bacterium community. The preparation method of the grease degrading bacterium community comprises the following steps: adding a deposit sample and grease collected from a food waste hogwash grease wastewater zone to an inorganic salt medium, and culturing the deposit sample and grease at 30DEG C under a rotating speed of 150r / min for 5d; repeatedly culturing the obtained material through the same technology 2 times to obtain a final liquid which is a bacterial liquid containing the grease degrading bacterium community; and carrying out DNA sequencing confirmation, wherein the grease degrading bacterium community comprises Stenotrophomonas maltophilia, Chryseobacterium spp, Achromobacter sp and enterobacteria. Onsite sampling (for achieving low cost) is realized, the grease degrading bacterium community is enriched and screened through a simple and convenient process, and grease wastewater in the food waste hogwash grease wastewater zone is effectively degraded; and the degradation efficiency in the treatment of the grease wastewater in the food waste hogwash grease wastewater zone under the action of a surfactant is greatly increased.

The invention relates to Achromobacter sp. capable of enduring zinc ion toxicity and application of the Achromobacter sp.. The invention provides the Achromobacter sp. capable of enduring zinc ion toxicity, and the preservation number of the Achromobacter sp. is CGMCC No. 2964. When the concentration of zinc ions in sewage is 0-50mg / L, the strain adopts nitrate nitrogen as a sole nitrogen source, and under an aerobiotic condition, the nitrate nitrogen removal rate is up to 100%, and the total nitrogen removal rate is as high as 100%. The strain has the advantages of being convenient and rapid to operate, high in denitrification efficiency, and the like, and has great significances for optimizing sewage biological treatment systems and alleviating influence of zinc ions upon sewage denitrification systems.

The invention discloses a strain capable of degradation of benzo[a]pyrene, and belongs to the technical field of biodegradation treatment. The benzo[a]pyrene degradation strain is identified as Achromobacter sp., and the strain preservation number is CGMCC3684. The strain is prepared into the bacterium suspension with the bacterium content of 1.25*10<8> CFU / ml. With the strain of the present invention, the degradation removal rates of the materials with the initial benzo[a]pyrene concentrations of 0.11 mg / L, 0.34 mg / L, 0.71 mg / L, and 1.19 mg / L are respectively 74.35%, 84.66%, 78.91% and 68.33%.

The invention belongs to the technical field of biochemistry and particularly relates to achromobacter sp. JA81 with the collection number of CCTCC M 2011369 and a method for obtaining an electron-deficient alkane compound by converting (Z)-3-aryl-3-cyan-crylic acid, annular imide and unsaturated nitro compounds by using the achromobacter as a biocatalyst. In the method, the maximum enantiomeric excess reaches over 99 percent and the highest conversion ratio reaches 100 percent. The catalyst achromobacter is easy to prepare and mild in reaction condition, and is one of the effective methods for preparing a chiral electron-deficient alkane compound in an environment-friendly manner.

The invention provides a degradative plasmid containing a functional gene capable of degrading fluoranthene, and an extraction method thereof. The degradative plasmid containing the functional gene capable of degrading fluoranthene is obtained by extraction and purification from a bacteria liquid of Achromobacter sp. The degradative plasmid containing the functional gene capable of degrading fluoranthene is obtained by the steps of inoculating Achromobacter sp. in a glass bottle with fluoranthene, a basal culture medium, a trace metal liquid and a vitamin C solution; culturing for 20 days to obtain the bacteria liquid; and extracting and purifying the bacteria liquid. The degradative plasmid containing the functional gene capable of degrading fluoranthene can be used for building a strain having a degrading function for fluoranthene, increases biodegradation effect of polycyclic aromatic hydrocarbon pollutants represented by fluoranthene and has application potentials for soil and water polluted by polycyclic aromatic hydrocarbons.

The invention discloses an achromobacter. The achromobacter is classified and named as Achromobacter sp, has a strain name of MBZ-2, is preserved in the China General Microbiological Culture Collection Center on August 13, 2020, and has a preservation number of CGMCC No. 20529. The achromobacter can effectively degrade p-toluenesulfonic acid and a downstream product herbicide quizalofop-p-ethyl.

The invention belongs to the technical field of environmental pollutant biological treatment, and specifically discloses achromobacter sp. MT-H used for degrading di-2-ethylhexylphthalate (DEHP). The achromobacter sp. MT-H is preserved at China Center for Type Culture Collection (CCTCC) on 22th, Jan., 2015, and preservation number is CCTCC No.M 2015058. The achromobacter sp. MT-H possesses relatively high degradation efficiency on DEHP, and is capable of degrading DEHP preferably in a relatively large pH and temperature range. It is confirmed that MT-H can be applied on DEHP polluted biological treatment as an excellent DEHP degrading bacterium.

Belonging to the bioremediation field of organic pollutants, the invention specifically discloses an Achromobacter sp. strain W143. The strain is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO:M2014154. The strain disclosed by the invention has efficient and fast degradation ability to phenanthrene, naphthalene, fluorene, fluoranthene and other organic pollutants. The strain also has wide pH value, salinity and temperature adaptability, and is an excellent material for bioremediation of soil or environmental polycyclic aromatic hydrocarbon organic pollutants.

The invention discloses an achromobacter sp. H380 for heavy metal cadmium pollution treatment. The achromobacter sp. H380 is preserved in the China center for type culture collection, and the preservation number is CCTCC NO: M2015291. According to the archromobacter, the heavy metal resistance spectrum is broad, the endurance capacity is strong, and the multi-antibiotic resistance is achieved, so that the environmental viability is strong, and the archromobacter is an excellent microbial material for heavy metal in-situ remediation. By means of the archromobacter, free cadmium ions in soil can be effectively removed, the acceptable daily intake of the cadmium ions for plants is obviously reduced, conversion from the free cadmium ions to combined cadmium ions is facilitated, therefore, the biological toxicity of the archromobacter is reduced, and the treatment of soil cadmium pollution is achieved.

The invention specifically discloses application of Achromobacter sp. MT-H in degradation of DEHP, which belongs to the technical field of biological treatment of environmental pollutants. The Achromobacter sp. MT-H is preserved in China Center for Type Culture Collection (CCTCC) on January 22, 2015, with an accession number of CCTCC M2015058. The Achromobacter sp. MT-H has high DEHP degradation efficiency and can perfectly degrade DEHP in wide pH and temperature ranges; thus, it is proved that MT-H can be used as an excellent DEHP degrading bacterium in biological treatment of DEHP pollution.

The present invention discloses isolation of Penicillin Acylase (PA) from Achromobacter sp CCM 4824 expressed in recombinant strain E. coli BL21 CCM 7394 bearing the recombinant plasmid pKXIP1 and processing of PA into biocatalyst useful for the industrial synthesis of antibiotics. More particularly the invention discloses a synthesis of semi-synthetic &bgr;-lactam antibiotics in the reaction mixture consisting of activated acyl-donor (D-p-hydroxyphenylglycine methyl ester or amide for Amoxicillin and Cefadroxil; D-phenylglycine methyl ester or amide for Ampicillin and Cephalexin) and nucleophile (6-APA or 7-ADCA) catalyzed by PA obtained from recombinant E. coli BL21 CCM 7394 as the biocatalyst.

The invention relates to an Achromobacter sanius strain JD417 and application thereof, the strain is preserved in the China Center for Type Culture Collection on July 2, 2021, and the preservation number is CCTCC NO: M 2021813. The invention also relates to an application of the Achromobacter sanius strain JD417. The strain JD417 can tolerate sulfonamide compounds (antibiotics) with high concentration (50mg / L), and not only can degrade the sulfonamide compounds, but also can degrade nitrogen substances. The strain can maintain survival by taking nitrate nitrogen as a sole nitrogen source and sulfamethazine as a sole carbon source, effectively reduces total nitrogen, nitrate nitrogen and sulfonamide compounds in a water body, and has good ecological efficiency and application prospects in the aspect of treatment of environmental pollution such as sewage and the like.

The present invention relates to mutants of penicillin G acylase from Achromobacter sp. CCM 4824 and uses thereof, and more specifically relates to Mutants of penicillin G acylase in a wild type penicillin G acylase protein sequence including an Alpha subunit shown in SEQ ID NO.1 and a Beta subunit shown in SEQ ID NO.2 and a method of preparing (synthesizing) Beta-Lactam antibiotics by using theMutants of penicillin G acylase, wherein the Mutants of penicillin G acylase select at least one mutation from a population consisting of A65AlphaV, A102AlphaE, A106AlphaT, D74BetaQ, R120BetaC, T176BetaS, Y180BetaS, T193BetaI, Y248BetaF, F254BetaY, T292BetaS, F343BetaY, E366BetaV and T485BetaS as the feature. The Mutants of penicillin G acylase from Achromobacter sp. CCM 4824, having a specific mutation form, are characterized by multiple syntheses with high efficiency for multiple semi-synthesized Beta-Lactam antibiotics.

The invention relates to an ethylbenzene degrading bacterium and a screening method and application thereof. The ethylbenzene degrading bacterium is Achromobacter, is named as Achromobacter sp.ED-2, and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO. M2021058, and the preservation time is January 14, 2021. The ethylbenzene degrading bacterium can completely degrade 100mg / L ethylbenzene within 48h, and can also degrade benzene, methylbenzene, m- and p-xylene and o-xylene. Through liquid chromatography detection, the bacterium has no intermediate product metabolic accumulation when degrading benzene, the degrading effect of the bacterium is stable, and support is provided for the biological purification practice process of benzene series polluted sites.

The present invention relates to a novel epoxide hydrolase enzyme which aims to achieve a high degree of resolution towards a broader range of substrates with high enantioselectivity and yields with minimal product inhibition. The invention further relates to a new bacterial strain Achromobacter sp. MTCC 5605 isolated from a petroleum-contaminated sludge sample, capable of producing the said enzyme. It is notable that the enzyme can be used as whole bacterial cell preparation, which allows continuous hydrolysis of substrates at even higher concentration and have an advantage of being recycled. The invention further relates to a process for the hydrolysis of different aryl epoxides which are potential synthons of intermediates for the synthesis of chiral amino alcohols and bioactive compounds like β-blockers.

The present invention relates to mutants of penicillin G acylase from Achromobacter sp. CCM 4824 and uses thereof, and more specifically relates to Mutants of penicillin G acylase in a wild type penicillin G acylase protein sequence including an Alpha subunit shown in SEQ ID NO.1 and a Beta subunit shown in SEQ ID NO.2 and a method of preparing (synthesizing) Beta-Lactam antibiotics by using the Mutants of penicillin G acylase, wherein the Mutants of penicillin G acylase select at least one mutation from a population consisting of A65AlphaV, A102AlphaE, A106AlphaT, D74BetaQ, R120BetaC, T176BetaS, Y180BetaS, T193BetaI, Y248BetaF, F254BetaY, T292BetaS, F343BetaY, E366BetaV and T485BetaS as the feature. The Mutants of penicillin G acylase from Achromobacter sp. CCM 4824, having a specific mutation form, are characterized by multiple syntheses with high efficiency for multiple semi-synthesized Beta-Lactam antibiotics.

The invention discloses a strain of achromobacter and its application in improving peanut iron nutrition. The achromobacter is named 1604IPR‑02, and the preservation number is CGMCC NO.16955. The Achromobacter 1604IPR-02 of the present invention is a gram-negative bacterium without spores, has a strong ability to produce siderophore, can convert insoluble iron in the soil into effective iron, can increase soil nutrients, and promote plant resistance to The absorption of soil iron, in particular, can successfully colonize the peanut rhizosphere soil, promote peanut growth and increase yield, and has great application potential.

The invention discloses a pyrene-degrading bacterium and its application, which belong to the technical field of biodegradation treatment. The pyrene-degrading bacterium was identified as Achromobacter sp., and the strain preservation number was CGMCC3637. The Genbank accession number of the 16S rDNA of this strain is EU220009.1. The bacterial strain is made into a bacterial suspension with a bacterial content of 1.25×108CFU / ml, and the initial concentration is respectively 0.11mg / l, 0.26mg / l, 0.52mg / l, and 1.14mg / l. The degradation of pyrene after 7 days The removal rates were 92.65%, 91.46%, 88.29%, and 89.67%, respectively.

The microbial pool provided by present invention is formed from Xanthomonas sp. dn 1 CGMCC No.1299, Flavobacterium sp.dn 2 CGMCC No. 1298, pseudomonas sp. dc 2 CGMCC No. 1297, Pseudomonas sp. dy1 CGMCC No. 1296 and Achromobacter sp. dy 3 CGMCC No. 1295. The above-mentioned microbial pool can be added into the soil polluted by oil, and can be used for degrading oil pollutant.