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Microbial agent for degrading gasoline petroleum hydrocarbons and using method of microbial agent

A microbial agent and petroleum hydrocarbon technology, applied in the field of environmental pollution control, can solve environmental pollution and other problems, and achieve the effect of complete degradation

Active Publication Date: 2017-12-01
UNIV OF SCI & TECH LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacterial agent is composed of Micrococcus, Pandora bacteria and Achromobacter. After being treated by the microbial bacterial agent, gasoline petroleum hydrocarbons can be degraded quickly and completely, which provides a solution for the environmental pollution of gasoline or other petroleum hydrocarbons. new ideas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] A microbial bacterial agent for degrading petroleum hydrocarbons is composed of Micrococcus JWJ-02, Pandora bacteria JWJ-06 and Achromobacter JWJ-09, wherein the number ratio of the three is 4:4:5.

[0083] The usage method of above-mentioned microbial inoculum is, under aseptic condition, add inorganic salt culture solution 100mL, trace element culture solution 0.1mL, gasoline 0.5mL in culture bottle, inoculate into the microbial inoculum of degrading gasoline petroleum hydrocarbon again, The total number of initial inoculations of Micrococcus, Pandora and Achromobacter were 6×10 6 pcs, 6×10 6 and 7.5×10 6 The culture flask was cultured on a shaker, the shaker speed was 120rad / min, the pH value of the culture medium was maintained at 6-7, the temperature was 29±1°C, and the culture time was 120h. The gasoline degradation rate was measured to be 87.6%.

[0084] According to GC-MS detection and analysis, the bacteria almost complete the conversion of chain alkanes, cyc...

Embodiment 2

[0092] A microbial bacterial agent for degrading petroleum hydrocarbons is composed of Micrococcus JWJ-02, Pandora bacteria JWJ-06 and Achromobacter JWJ-09, wherein the number ratio of the three is 4:2:4.

[0093] The method of using the above-mentioned microbial inoculum is as follows: under aseptic conditions, add 0.1 mL of trace element culture solution to 100 mL of inorganic salt medium, add 1.2 mL of gasoline, and then inoculate into the microbial inoculum for degrading gasoline-like petroleum hydrocarbons, Micrococcus , Pandora, and Achromobacter inoculations were 3.6×10 7 pcs, 1.8×10 7 and 3.6×10 7 On a shaker, the shaker speed is 120rad / min, the pH value of the culture medium is maintained at 7-8, the temperature is 31±1°C, and the incubation time is 120h. The gasoline degradation rate is measured to be 80.9%.

Embodiment 3

[0095] A microbial bacterial agent for degrading petroleum hydrocarbons is composed of Micrococcus JWJ-02, Pandora bacteria JWJ-06 and Achromobacter JWJ-09, wherein the number ratio of the three is 4:3:4.

[0096] The use method of the above-mentioned microbial inoculum is as follows: under aseptic conditions, add 0.05 mL of trace element culture solution to 100 mL of inorganic salt medium, add 0.35 mL of gasoline, and then inoculate into the microbial inoculum for degrading gasoline petroleum hydrocarbons, Micrococcus , Pandora, and Achromobacter were inoculated with a total number of 2.8×10 7 pcs, 2.1×10 7 and 2.8×10 7 On a shaker, the shaker speed is 120rad / min, the pH value of the medium is maintained at 7-8, the temperature is 30±1°C, and the incubation time is 96h. The gasoline degradation rate is measured to be 81.7%.

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Abstract

The invention aims at developing a microbial method for processing gasoline petroleum hydrocarbons, and provides a microbial agent for degrading petroleum hydrocarbons and a using method of the microbial agent. The microbial agent is composed of Micrococcus sp., Pandoraea sp. and Achromobacter sp., preferably, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. in the microbial agent is (8-16): (5-12): (10-16), or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 4:4:5; or, the number ratio of the Micrococcus sp. to the Pandoraea sp. to the Achromobacter sp. is 2:1:2. According to the using method of the microbial agent for degrading the petroleum hydrocarbons, the microbial agent is used for degrading gasoline petroleum hydrocarbons, wastewater containing the gasoline petroleum hydrocarbons, or soil containing the gasoline petroleum hydrocarbons. After being treated by the microbial agent, the gasoline petroleum hydrocarbons can be rapidly and completely degraded, and thus a new idea is provided for solving the environmental pollution problem caused by gasoline or other petroleum hydrocarbons.

Description

technical field [0001] The invention belongs to the field of environmental pollution control, and in particular relates to a microbial bacterial agent for degrading petroleum hydrocarbons and a use method thereof. Background technique [0002] With the continuous development of industry, petroleum is used as one of the main energy sources and one of the main raw materials, appearing in all walks of life. However, in the process of oil exploitation, transportation, processing, etc., due to problems such as leakage and wrong discharge, soil, rivers, groundwater, oceans, etc. are polluted by oil, which brings pollution to the ecological environment. [0003] The remediation technologies for petroleum hydrocarbon pollution mainly include physical, chemical and microbial methods. Compared with physical remediation and chemical remediation, microbial remediation technology is a relatively mature technology, and has the advantages of safety, high efficiency, low cost, simple opera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C09K17/14A62D3/02A62D101/20C12R1/265C12R1/025C12R1/01C02F101/32
CPCA62D3/02A62D2101/20B09C1/10C02F3/34C02F2101/32C09K17/14C12N1/02C12N1/20C12N1/205C12R2001/01C12R2001/025C12R2001/265
Inventor 金文杰郝瑞霞齐欣李圆雷春燕王娜卢志超朱任杰
Owner UNIV OF SCI & TECH LIAONING
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