Achromobacter and application thereof in degradation of p-toluenesulfonic acid and quizalofop-p-ethyl

A technology of p-toluenesulfonic acid and Achromobacter, applied in the biological field, can solve problems such as environmental pollution, pollution, ecological damage and the like

Active Publication Date: 2021-03-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although quizalofop-p-ethyl is considered to be a low-toxicity pesticide, its widespread use and a large amount of residue and accumulation in the environment have caused global environmental pollution and ecological damage in addition to directly affecting the soil environment, water environment and atmospheric environment. In addition to causing pollution, quizalofop-ethyl will also accumulate in organisms through the food chain, which will have a serious impact on the safety of humans and animals

Method used

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  • Achromobacter and application thereof in degradation of p-toluenesulfonic acid and quizalofop-p-ethyl
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  • Achromobacter and application thereof in degradation of p-toluenesulfonic acid and quizalofop-p-ethyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation of Achromobacter sp MBZ-2

[0032] Sludge samples were collected from the activated sludge tank of the wastewater treatment plant of a pesticide production enterprise in Jiangsu, enriched and cultivated by continuous enrichment culture method, and the degradation effect was tested by high performance liquid chromatography, and the effective enrichment solution was diluted and coated in the containing 0.5g / L solid inorganic salt of p-toluenesulfonic acid (culture medium formula: p-toluenesulfonic acid 0.5g / L, NH 4 Cl 1.0g / L, KH 2 PO 4 0.6g / L, K 2 HPO 4 1.3g / L, NaCl 0.5g / L, MgSO 4 ·7H 2 O0.2 g / L, pH7.0. ) culture medium and cultured in a constant temperature incubator at 30°C until colonies grew. According to the size, color, and shape of the colonies, pick different single colonies to streak and purify. The strain whose degradation efficiency was 98.4% after 72 hours was selected as MBZ-2.

Embodiment 2

[0033] Embodiment 2: Degradation effect determination of quizalofop-ethyl by Achromobacter sp MBZ-2

[0034] Achromobacter sp (Achromobacter sp) MBZ-2 was streaked on the LB solid plate, and then a single colony was picked from the LB plate, inoculated in LB liquid medium, and cultivated in a shaker at 37°C and 180rpm until OD600= After centrifugation at 1.0, 10000rpm, the supernatant was discarded, washed three times with sterile water, and resuspended in an equal volume of sterile water as the seed solution. Inoculate the MBZ-2 seed solution with an inoculation amount of 1vt% in an inorganic salt medium with a quizalofop-p-ethyl concentration of 50mg / L, cultivate it at 37°C in a 180rpm shaker, and measure quizalofop-pyl-ethyl in the solution after sampling and filtering every 4h Heling concentration, the effect is as image 3 Shown, degradation curve see Figure 4 .

Embodiment 3

[0035] Embodiment 3: Degradation effect determination of p-toluenesulfonic acid by Achromobacter sp MBZ-2

[0036] Achromobacter sp (Achromobacter sp) MBZ-2 was streaked on the LB solid plate, and then a single colony was picked from the LB plate, inoculated in LB liquid medium, and cultivated in a shaker at 37°C and 180rpm until OD600= After centrifugation at 1.0, 10000rpm, the supernatant was discarded, washed three times with sterile water, and resuspended in an equal volume of sterile water as the seed solution. Inoculate the MBZ-2 seed solution with an inoculation amount of 1vt% in an inorganic salt medium with a p-toluenesulfonic acid concentration of 500mg / L, cultivate it in a shaker at 37°C and 180rpm, and measure the concentration of the solution after sampling and filtering every 4h. Concentration of p-toluenesulfonic acid, the effect as figure 2 Shown, degradation curve see Figure 5 .

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Abstract

The invention discloses an achromobacter. The achromobacter is classified and named as Achromobacter sp, has a strain name of MBZ-2, is preserved in the China General Microbiological Culture Collection Center on August 13, 2020, and has a preservation number of CGMCC No. 20529. The achromobacter can effectively degrade p-toluenesulfonic acid and a downstream product herbicide quizalofop-p-ethyl.

Description

technical field [0001] The invention relates to the biological field, in particular to an achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop-p-ethyl. Background technique [0002] p-toluenesulfonic acid (PTS for short), its molecular formula is: C 7 h 8 o 3 S, the structural formula is shown in formula I. p-Toluenesulfonic acid is white needle-like or powdery crystal, melting point 106-107°C, boiling point 140°C / 2.67kPa, easily soluble in water, alcohol, ether and other polar solvents, insoluble in benzene, toluene and other non-polar solvents It is a non-oxidizing organic strong acid, and its acidity is 1 million times that of benzoic acid. It is an important raw material for chemical synthesis, and is widely used as an intermediate in the synthesis of medicines (such as naproxen, doxycycline, etc.), pesticides (such as quizalofop-p-ethyl, dicofol, etc.) and dyes (acid orange 7, etc.) ; It is also used as a catalyst for the synthesis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/36C02F101/30
CPCC12N1/20C02F3/34C02F2101/40C02F2101/36C02F2101/306
Inventor 蔡天明蔡舒陈立伟
Owner NANJING AGRICULTURAL UNIVERSITY
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