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Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP)

A technology of diisooctyl phthalate and Achromobacter, which is applied in the application field of Achromobacter MT-H in degrading diisooctyl phthalate, and can solve the problem that the degradation rate cannot meet the actual pollution control Questions such as requirements

Active Publication Date: 2015-08-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of the reported strains to DEHP can not meet the requirements of actual pollution control, and there are few reports on the germplasm resources of Achromobacter that can degrade DEHP

Method used

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  • Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP)
  • Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP)
  • Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The isolation and identification of embodiment 1 bacterial strain

[0029] The return sludge of Datansha Urban Wastewater Treatment Plant in Guangzhou was collected, and 5 g of activated sludge samples were weighed in a 150 mL Erlenmeyer flask containing 50 mL of sterile water. Mud suspension was added to 100 mL containing DEHP (50 mg L -1 ) of the above MSM medium. After culturing at 30°C and 140 rpm for 7 days, the inoculum size of 1 mL was continuously enriched and transferred 10 times, and the content of DEHP in the medium was correspondingly increased to 1000 mg L -1 . Then the culture medium transferred 10 times was diluted 10 3 ~10 5 Double-spread on LB solid plates and incubate upside down at 30°C for 1-3 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a strain of bacteria, numbered MT-H. Then the strains were inoculated on LB solid plates and cultured upside down at 30°C for ...

Embodiment 2

[0033] Example 2 Achromobacter Experiment on the degradation effect of sp.MT-H on DEHP

[0034] S1. Bacterial suspension preparation: insert the purified strain MT-H into LB liquid medium containing 10 mL and activate overnight to the logarithmic phase, collect the bacteria by centrifugation at 5000 rpm for 10 min, and wash the bacteria 3 times with PBS After resuspension, adjust OD 600 nm = 1.0 as the strain suspension.

[0035] S2. Degradation performance measurement: 100mL containing different concentrations of DEHP (50, 100, 200, 400, 600 mg L -1 ) was inoculated with 1 mL of MSM culture solution, and no bacteria were used as a control, and the pH was adjusted to 8.0, and each group was repeated three times. Incubate at 30°C and 140 rpm constant temperature shaker for 9 days, take samples regularly, and measure the degradation of DEHP by GC / MS.

[0036] Chromatographic conditions: Shimadzu QP2010 Plus GC / MS tandem mass spectrometer was used. The chromatographic...

Embodiment 3

[0040] Embodiment 3 pH, temperature and rotating speed pair Achromobacter Effect of sp. MT-H on DEHP degradation

[0041] pH pair Achromobacter Influence of sp.MT-H on DEHP degradation: 100 mL of MSM culture solution containing DEHP (500 mg / L) with different pH (5, 6, 7, 8, 9, 10) was inoculated with 1 mL of bacteria, and no bacteria were used as For the control, three repetitions were performed in each group. After culturing in a constant temperature shaker at 30°C and 140 rpm for 48h, the degradation of DEHP was determined by GC / MS.

[0042] temperature pair Achromobacter The effect of sp.MT-H on DEHP degradation: Inoculate 2.5mL of bacteria into 100mL of MSM culture solution containing DEHP (500mg / L), and use no bacteria as a control. ℃, 30℃, 35℃, 40℃ in a constant temperature shaker at 140 rpm for 48h, the degradation of DEHP was determined by GC / MS.

[0043] speed pair Achromobacter The effect of sp. MT-H on the degradation of DEHP: Inoculate 2.5mL of bacteria int...

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Abstract

The invention specifically discloses application of Achromobacter sp. MT-H in degradation of DEHP, which belongs to the technical field of biological treatment of environmental pollutants. The Achromobacter sp. MT-H is preserved in China Center for Type Culture Collection (CCTCC) on January 22, 2015, with an accession number of CCTCC M2015058. The Achromobacter sp. MT-H has high DEHP degradation efficiency and can perfectly degrade DEHP in wide pH and temperature ranges; thus, it is proved that MT-H can be used as an excellent DEHP degrading bacterium in biological treatment of DEHP pollution.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to Achromobacter ( Achromobacter sp.) Application of MT-H in degradation of di-isooctyl phthalate. Background technique [0002] Phthalate, also known as phthalate, abbreviated as PAEs, is a general term for esters formed by phthalic acid. Among them, diisooctyl phthalate (DEHP) is a typical phthalate ester, which is widely used in people's daily life, especially as a plastic additive, which can reach 20-50% by weight . Since DEHP in plastic products is easily transferred into the environment, it widely exists in the atmosphere, water, soil and organisms. DEHP is the most toxic among phthalates. It can enter the organism through various channels, seriously interfere with its endocrine, reproductive and nervous systems, and has strong teratogenicity, mutagenicity, and carcinogenicity. At present, DEHP has become one of the most common pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/025C02F101/34A62D101/28
Inventor 赵海明莫测辉蔡全英李彦文李慧林静杜欢陈学斌
Owner JINAN UNIVERSITY
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