Achromobacter sp. and application thereof to improvement of iron nutrition of peanuts

A colorless bacillus and bacterial agent technology, applied in the field of microbiology and plant growth-promoting bacteria, can solve the problems of single type of siderophore and growth-promoting effect, increase the dry weight and yield of peanuts, have good application prospects, and increase the relative expression volume effect

Active Publication Date: 2020-01-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common siderophore-producing bacteria in the prior art are mostly Pseudomonas and Bacillus...

Method used

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  • Achromobacter sp. and application thereof to improvement of iron nutrition of peanuts
  • Achromobacter sp. and application thereof to improvement of iron nutrition of peanuts
  • Achromobacter sp. and application thereof to improvement of iron nutrition of peanuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Achromobacter sp. screening, isolation and identification

[0051] 1. Screening and isolation of bacterial strains:

[0052] Achromobacter 1604IPR-02 was isolated from the intercropping peanut rhizosphere soil in the peanut-corn intercropping system. The soil samples were resuspended in sterile water and cultured on CAS solid medium. The strains with strong siderophore production ability were selected and obtained. After further screening and breeding, the achromobacter was obtained, and the purified bacterium was named 1604IPR-02.

[0053] 2. Determination of the morphology, physiological and biochemical characteristics of strain 1604IPR-02

[0054] (1) Colony characteristics and cell morphology The colony and cell characteristics of strain 1604IPR-02 are: the colony is round, with a raised center, light yellow color, smooth and neat edges, and the cell is moist and translucent. The morphological characteristics of the microscopic observation body were rod-...

Embodiment 2

[0059] Embodiment 2 Achromobacter 1604IPR-02 growth curve and siderophore curve determination

[0060] (1) Growth curve measurement

[0061] Take 12 Erlenmeyer flasks filled with 50ml sterile LB culture solution, numbered 0, 3, 6, 12, 24, 30, 36, 48, 60, 72, 96, 120 respectively. Achromobacter 1604IPR-02 was inoculated into 12 numbered Erlenmeyer flasks with 1% inoculation amount, and cultured with shaking at 30°C at a speed of 180r / min. Then take out the Erlenmeyer flask according to the corresponding time to measure the OD value. Pour the uninoculated LB culture solution into the cuvette, adjust the zero point on the 600nm wavelength spectrophotometer as a blank control, and measure the culture solution at different times from 0h in sequence, and use the uninoculated solution for the bacterial suspension with high concentration. The inoculated LB liquid medium was properly diluted and measured so that the OD value was within 0.10.-0.65. The OD value measured after diluti...

Embodiment 3

[0074] Example 3 Effect of Achromobacter 1604IPR-02 on peanut growth and iron nutrition

[0075] 1. Bacterial strain 1604IPR-02 of the present invention was cultivated in LB liquid medium at 30°C to 10 9 cfu / ml, centrifuge at 5000rpm for 10min in a high-speed centrifuge, pour off the supernatant, add an equal amount of sterilized 10g / l Nacl distilled aqueous solution to prepare a bacterial suspension, and set aside.

[0076] On May 16, 2018, peanuts were transplanted in the greenhouse of the College of Resources and Environment, China Agricultural University, Beijing. The peanut variety was Luhua 14. And from the first week after the thinning, add the bacterial solution at a rate of 20ml / plant every week, and the concentration of the bacterial solution in the bacterial addition treatment: 1×10 9CFU / ml, the same amount of sterile saline solution with the same concentration was added to the control group. Two groups of treatments each had 4 pots, and each pot had 2 bio...

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Abstract

The invention discloses achromobacter sp. and an application thereof to improvement of iron nutrition of peanuts. The achromobacter sp. is named as 1604IPR-02, and the preservation number is CGMCC NO.16955. The achromobacter sp. 1604IPR-02 is a gram-negative bacterium, does not have spores, has high siderophore production capacity, can convert ferrum which is slightly soluble in soil into effective ferrum, can increase soil nutrients, can promote absorption of the ferrum in the soil by plants, particularly can be successfully colonized in peanut rhizosphere soil, can promote growth of the peanuts, can increase yield, and has excellent application potential.

Description

technical field [0001] The invention belongs to the field of microbiology and plant growth-promoting bacteria, and in particular relates to an achromobacter with high siderophore production capacity and improved plant iron nutrition and application thereof. Background technique [0002] Peanut is not only one of the main edible oil crops in my country, but also an important raw material for the food and oil processing industry and a high-quality substitute for margarine and olive oil. my country is a large peanut producing country, which plays an important role in the development of our national economy. Iron is one of the essential elements for various organisms, and it participates in important metabolic activities in organisms. Physiological metabolic processes such as photosynthesis, respiration, and DNA synthesis of plants all require the participation of elemental iron. However, most of the iron in the soil exists in the form of insoluble iron oxide, and its bioavail...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/20A01P21/00C12R1/025
CPCA01N63/00C12N1/20C12P1/04C12N1/205C12R2001/025Y02A40/10
Inventor 左元梅王天琪李士芹王男麒
Owner CHINA AGRI UNIV
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