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A kind of Pseudomonas fluorescens in peanut rhizosphere and its application

A technology of Pseudomonas fluorescens and a bacterial agent, which is applied in the fields of microbiology and plant growth-promoting bacteria, and achieves the effects of good application prospects, enhanced salt stress resistance, and improved peanut biomass and yield.

Active Publication Date: 2021-10-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are few reports about Pseudomonas fluorescens improving peanut iron, nitrogen, and potassium. Pseudomonas fluorescens with trophic effects has not been reported yet

Method used

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  • A kind of Pseudomonas fluorescens in peanut rhizosphere and its application
  • A kind of Pseudomonas fluorescens in peanut rhizosphere and its application
  • A kind of Pseudomonas fluorescens in peanut rhizosphere and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Pseudomonas fluorescens (Pseudomonas fluorescens) screening, isolation and identification

[0041] 1. Screening and isolation of bacterial strains:

[0042] CAS screening medium

[0043] Preparation of CAS plate staining solution: Dissolve CAS 0.0605g in 40mL deionized water, CTAB 0.1458g in 50mL, 10mM FeCl 3 1mL, dilute to 100mL.

[0044] Soil samples were corn-peanut intercropping peanut rhizosphere soil. The peanut rhizosphere soil was carefully shaken off into a sterile bag, placed in a low-temperature fresh-keeping box, and processed immediately after being brought back to the laboratory. Take 5g of rhizosphere soil sample and put it into 45mL 0.9% NaCl sterile water, put it in a shaking incubator for 10min and mix well, take the supernatant to get the soil microbial suspension, carry out serial gradient dilution, and absorb 100μL of the diluted microbial suspension Spread evenly on a solid CAS plate with a spreader, seal the plate with parafilm, and...

Embodiment 2

[0052] Example 2 Production of strain siderophores and verification of iron-dissolving ability

[0053] 1. Materials and methods

[0054] (1) Determination of bacterial growth curve

[0055] According to the method of Chen Shaoxing et al., the medium for inducing siderophore was improved MSA medium, and the specific formula was: 20 g of sucrose, 2 g of aspartic acid, K 2 HPO 4 1g, MgSO 4 0.5g, distilled water 1000mL. The culture conditions were 30°C, 200 rpm, samples were taken at regular intervals, the OD600 of the bacterial solution was measured, and the growth curve was drawn.

[0056] (2) Qualitative detection of siderophores

[0057] Preparation of CAS liquid detection solution: refer to the method of Schwyn & Neilands, respectively prepare 2mM CAS stock solution, 1mM FeCl 3 Stock solution, 0.2M 5-sulfosalicylic acid stock solution.

[0058] CTAB solution: 0.0219g dissolved in 25mL ultrapure water.

[0059] Piperazine buffer solution: Weigh 4.3079 g of anhydrous...

Embodiment 3

[0069] Example 3 Verification test of auxin produced by strain 1502IPR-01

[0070] (1) Qualitative determination of IAA secretion by 1502IPR-01 strain

[0071] 1502IPR-01 was inoculated into LB liquid medium containing 100mg / L L-tryptophan, placed in a constant temperature shaker at 28°C and shaken at 180r / min for 3 days. Draw 50 μL of the bacterial suspension and drop it on a white ceramic plate, and add 50 μL of Salkowski colorimetric solution. At the same time, 50 mg / L of IAA was added to Salkowski colorimetric solution as a positive control. Store the white ceramic plate at room temperature in the dark for 30 minutes, and observe the color change. If the color turns red, it means that the strain has the function of producing IAA. The configuration method of Salkowski colorimetric solution: the configuration volume fraction is 35% HClO 4 50mL, the configuration concentration is 0.5mol / L FeCl 3 1mL, mix well and store away from light.

[0072] (2) Quantitative determ...

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Abstract

The invention discloses a peanut rhizosphere Pseudomonas fluorescens and application thereof. The Pseudomonas fluorescens is named 1502IPR‑01, and the preservation number is CGMCC NO.16925. The Pseudomonas fluorescens 1502IPR-01 in the peanut rhizosphere of the present invention is a Gram-negative bacterium without spores, has a strong ability to produce siderophores, and the produced siderophores have better ability to chelate insoluble iron. Pseudomonas fluorescens 1502IPR‑01 and its bacterial agent have high siderophore production capacity, significantly increase the content of active iron in peanut new leaves, improve peanut iron nutrition, produce auxin to promote lateral root development, improve root architecture, and promote nitrogen and phosphorus in plants. The increase of potassium content reduces the sodium content of plants, improves resistance to salt stress, significantly promotes peanut growth, improves peanut biomass and yield and other growth-promoting functions. The Pseudomonas fluorescens 1502IPR-01 of the present invention has a positive effect on plant growth promotion.

Description

technical field [0001] The invention belongs to the field of microbiology and plant growth-promoting bacteria, and specifically relates to a kind of high-yield siderophore, high iron-dissolving ability, producing auxin, promoting lateral root growth, improving plant iron nutrition and nitrogen, phosphorus and potassium element nutrition, and enhancing plant growth. Salt stress tolerance and plant growth promotion Pseudomonas fluorescens and its application. Background technique [0002] Iron is one of the essential elements for various organisms, and it participates in important metabolic activities in organisms. Physiological metabolic processes such as photosynthesis, respiration, and DNA synthesis of plants all require the participation of elemental iron. However, most of the iron in the soil exists in the form of insoluble iron oxide, and its bioavailability is low, especially on calcareous soils, the high soil pH results in extremely low soluble iron content, so plants...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P21/00C05G3/00C12R1/39
CPCA01N63/00C05F11/08C05G3/00C12N1/20C12N1/205C12R2001/39
Inventor 左元梅李士芹王天琪王男麒
Owner CHINA AGRI UNIV
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