Corn endophyte Bacillus subtilis and its application in biocontrol

A technology of bacillus and subtilis, applied in the direction of application, bacteria, biocide, etc., can solve the problem of unstable control effect, and achieve the effect of increasing the vitality of the root system

Inactive Publication Date: 2015-04-15
浙江省东阳玉米研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on corn biological control at home and abroad is basically only aimed at a single disease, and the control effect is unstable. Therefore, those skilled in the art urgently need the emergence of new multifunctional microbial preparations that can effectively control corn-related diseases.

Method used

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  • Corn endophyte Bacillus subtilis and its application in biocontrol
  • Corn endophyte Bacillus subtilis and its application in biocontrol
  • Corn endophyte Bacillus subtilis and its application in biocontrol

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Effect test

Embodiment 1

[0016] Embodiment 1: Isolation and identification of Bacillus subtilis BS0521

[0017] Bacterial strain Bacillus subtilis BS0521 of the present invention is isolated from sweet corn seed, and specific operation mode is as follows:

[0018] (1) Sample treatment: Prepare Zhefengtian No. 2 corn seeds, rinse with clean water, and soak in sterile water at 30°C for 30 minutes;

[0019] (2) Surface sterilization: after rinsing with sterile water, place in 95% alcohol for 3 minutes, rinse with sterile water for 4 times immediately after disinfection, and take 120 μL of the last rinse water and apply it to NA medium (beef extract 1g, peptone 5g, yeast extract 5g, sodium chloride 5g, sucrose 10g, agar 20g, water 1000mL), culture at 28°C for 72h, and test the surface sterilization effect;

[0020] (3) Separation: Use sterilized scissors to cut the sterile material on the surface into small pieces, transfer it to a centrifuge tube filled with 1mL sterile water, shake it fully, absorb 0.2...

Embodiment 2

[0028] Example 2: Effect of Bacillus subtilis BS0521 on corn root activity and biomass

[0029] The BS0521 strain was inoculated on the NA medium by the three-section line method, and a single colony was picked and inoculated in the NB liquid medium, cultured at 30°C with shaking at 170r / min, overnight, and ready for use. The BS0521 bacterial suspension was centrifuged at 10 000 r / min for 20 min, the supernatant was carefully drawn, and sterilized by filtration with a 0.22 μm microporous membrane to obtain a BS0521 sterile filtrate for use.

[0030] Soak corn seeds with BS0521 bacterial suspension and sterile filtrate for 30 minutes, sow the seeds, and soak the seeds in clear water as a control. Each treatment was replicated 5 times with 30 seeds per replicate. The emergence rate was counted 7 days after emergence. Harvest corn plants with roots, wash the soil around the roots of the plants, and absorb the water. Root activity of maize seedlings was determined by the method...

Embodiment 3

[0034] Embodiment 3: broad-spectrum determination of Bacillus subtilis BS0521 antibacterial activity

[0035] The pathogenic target bacteria for the broad-spectrum assay of the antibacterial activity of Bacillus subtilis BS0521 are Pseudomonas grandis, Pseudomonas minor, Stem rot and Sheath blight. These four diseases are the main leaf spot diseases and soil-borne diseases on maize plants at present. The specific operation method is as follows: the pathogenic bacteria are cultured in a straight line in the middle of the PDA medium, and BS0521 is inoculated on both sides of the straight line (such as figure 1 Shown), with only the treatment of inoculating pathogenic bacteria as the control, each treatment was repeated 4 times, the growth and changes of the colonies were observed, and the degree of antagonism was judged by the size of the inhibition zone.

[0036] The results showed that BS0521 had significant inhibitory effect on maize leaf spot, small leaf spot and sheath bli...

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Abstract

The invention discloses a corn endophyte Bacillus subtilis and its application in biocontrol, and belongs to the technical field of corn disease control. The strain Bacillus subtilis BS0521 provided by the invention is preserved in China Center for Type Culture Collection on November 16, 2014, and has a preservation number of CCTCC No: M 2014566; and the preservation address is: Wuhan University, Wuhan, China. The strain of the invention has growth promoting effect on healthy corn seedlings, also can increase the root vigor, and can be used as a biofertilizer for corn seedling stage; the strain under in vitro condition has inhibition effect on pathogens of a plurality of corn diseases, can be used as a biotype seed coating agent in the effective prevention and treatment of sheath blight, also can be used as a resistance inducer for corn southern leaf blight; therefore, the strain can be used in the control of corn sheath blight and induced resistance to corn southern leaf blight.

Description

technical field [0001] The invention belongs to the technical field of corn disease prevention and control, and in particular relates to an endophytic Bacillus subtilis in corn and its biocontrol application. Background technique [0002] Corn is one of the main food crops in my country, ranking third in planting area and total output after wheat and rice. In recent years, in addition to being used for food, corn is also used as high-quality feed for the development of animal husbandry and an important raw material for light industry and pharmaceutical industry. Therefore, corn plays an important role in the development of the national economy. [0003] In recent years, with climate changes, changes in cultivation systems and varieties, and large-scale planting of high-starch varieties, soil-borne diseases of maize, especially sheath blight, have been increasing year by year. In normal years, the incidence rate is about 10%-30%, and in severe years, it can even reach more t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 苏婷王桂跃韩海亮赵福成谭禾平包斐
Owner 浙江省东阳玉米研究所
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