A kind of dead valley bacillus and application thereof

A Bacillus of death valley, seed tank technology, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as environmental pollution and human health threats

Active Publication Date: 2017-01-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and continuous use of chemical pesticides has caused a series of serious consequences, such as killing the natural enemies of pests, causing germs and pests to develop resistance, seriously polluting the environment, and threatening human health.
There is no patent report on the use of Bacillus dead valley to control fungal diseases of ginseng

Method used

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  • A kind of dead valley bacillus and application thereof
  • A kind of dead valley bacillus and application thereof
  • A kind of dead valley bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068]Isolation and Preservation of Bacillus vallismortis SZ-4 Strain

[0069] The strain is isolated from the soil within 30cm around the rhizosphere of perennial ginseng in the ginseng cultivation base of Wanliang Town, Fusong County, Jilin Province. The above soil samples were collected, air-dried and sieved. Weigh 10 g of the sample, put it into a conical flask filled with glass beads and 90 mL of sterile water, shake it fully for 10-30 minutes, mix the sample with sterile water evenly, and prepare a sample suspension, and let it stand for 5 minutes. Take 1mL of supernatant under sterile conditions, add 9mL of 0.05% SDS aqueous solution (sodium dodecyl sulfate aqueous solution), keep warm at 40°C for 20min, take 1mL, add 9mL of sterile water, and make 10 -3 、10 -4 、10 -5 diluent. Pipette 100 μL of each dilution and add to beef extract peptone (NB) plate, spread evenly by plate dilution method, repeat each treatment 3 times, and incubate in a 34°C incubator for 1-2 days...

Embodiment 2

[0074] Inhibitory effect of Bacillus dead valley SZ-4 strain on the growth of pathogenic fungi of ginseng fungal diseases

[0075] The antagonism of strain SZ-4 to ginseng fungal disease pathogens was determined by the filter paper method: the activated ginseng pathogenic bacteria colonies were made into bacteria cakes of corresponding sizes with a puncher with a diameter of 8 mm, and aseptically inoculated on potato dextrose agar (PDA) At the very center of the flat plate (diameter 90 mm), four sterilized filter paper discs with a diameter of 1 cm were glued to the four corners at a distance of 25 mm from the center of the flat plate. Bacterial strain SZ-4 was made bacterial suspension (concentration is about 10 8 cfu / mL), in which 3 pieces of filter paper discs were spotted with 20 μL of bacterial suspension, and 1 piece of filter paper discs was spotted with 20 μL of sterile water, as a treatment group, and each treatment was repeated 3 times; The 4 filter paper disks at t...

Embodiment 3

[0084] Plate confrontation experiment of Bacillus death valley SZ-4 against ginseng pathogenic bacteria

[0085] Adopt the Oxford cup method to measure the antagonistic activity of bacterial strain SZ-4 to ginseng fungal disease pathogen: collect after SZ-4 bacterial strain is made bacterial suspension (preparation method is the same as example 2), under the condition of 4 ℃, 12000r / min centrifugal 20min, The collected supernatant was filtered through a 0.22 μm microporous membrane, and the obtained filtrate was stored at 4°C for future use. Inoculate a ginseng germ cake with a diameter of 8mm in the center of a PDA plate (diameter 90mm) under sterile conditions, then place 4 sterile Oxford cups at 4 symmetrical corners about 25mm away from the center of the plate, and add 100μl dropwise to each cup The filtrate was repeated three times for each treatment, and the width of the inhibition zone was measured after culturing at 28°C for 5 days. The results showed that the inhibit...

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Abstract

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

Description

technical field [0001] The invention relates to a Bacillus dead valley and application thereof. Background technique [0002] Ginseng (Panax ginsengC.A.Mey) is a perennial root-negative dicotyledonous medicinal plant of Araliaceae Panax. It is a precious medicinal material in my country and has the reputation of "the king of herbs". Ginseng is planted in large areas in China, South Korea, North Korea, Russia and other countries. Among them, Northeast China is the main production area of ​​ginseng and has become one of the important pillar industries in the local area. The long-term artificial planting and variety selection of ginseng is difficult, which leads to the degradation of ginseng germplasm, serious diseases, poor quality and low yield. Extensive use of chemical pesticides leads to pesticide residues and environmental pollution, which reduces the safety and commodity value of ginseng medicinal materials. Disease prevention and control has become one of the major is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 杨利民孙卓韩梅韩忠明
Owner JILIN AGRICULTURAL UNIV
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