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Microbial remediation method for petroleum-contaminated soil

A technology for microbial remediation and oil pollution. It is applied in the restoration of polluted soil and biological sludge treatment. It can solve the problems of difficult treatment, complex sludge composition and high toxicity.

Inactive Publication Date: 2017-05-31
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is characterized by a large polluted area and complex composition of sludge, including water, petroleum, heavy metals, clay, sand and gravel, etc. It is difficult to treat and highly toxic

Method used

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  • Microbial remediation method for petroleum-contaminated soil
  • Microbial remediation method for petroleum-contaminated soil
  • Microbial remediation method for petroleum-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] a. Collect the oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0020] b. Add biomass powder with a particle size of 1 mm to the soil in step a by 10% by volume to mix evenly as cotton stalk powder, and adjust the moisture content to 20%, and adjust the pH to 7.5;

[0021] c. Preparation of surfactant crude product: change the inorganic salt medium to NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, add 4g of n-hexadecane, sterilize at 121°C for 20min, insert Pseudomonas aeruginosa liquid Pseudomonasaeruginosa after cooling, and use a ventilating Seal the membrane, place it in a constant temperature shaker at a temperature of 30°C, and incubate at 120r / min for 96 hours to obtain a crude surfactant with a solution surface tension of 26.68mN / m;

[0022] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a vol...

Embodiment 2

[0025] a. Collect the oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0026] b. Add biomass powder with a particle size of 2mm to the soil in step a by 20% by volume and mix it uniformly as sawdust, and adjust the moisture content to 25%, and adjust the pH to 7.5;

[0027] c. Preparation of surfactant crude product: change the inorganic salt medium to NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, add 4g of n-hexadecane, sterilize at 121°C for 20min, insert Pseudomonas aeruginosa liquid Pseudomonasaeruginosa after cooling, and use a ventilating Seal the membrane, place it in a constant temperature shaker at a temperature of 30°C, and incubate at 120r / min for 96 hours to obtain a crude surfactant with a solution surface tension of 26.68mN / m;

[0028] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a volume ra...

Embodiment 3

[0031] a. Collect the oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0032] b. adding biomass powder with a particle size of 3 mm to the soil in step a by volume ratio of 30% to be rice husk and mix evenly, and adjust the moisture content to 30%, and adjust the pH to 7.5;

[0033] c. Preparation of surfactant crude product: change the inorganic salt medium to NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, add 4g of n-hexadecane, sterilize at 121°C for 20min, insert Pseudomonas aeruginosa liquid Pseudomonasaeruginosa after cooling, and use a ventilating Seal the membrane, place it in a constant temperature shaker at a temperature of 30°C, and incubate at 120r / min for 96 hours to obtain a crude surfactant with a solution surface tension of 26.68mN / m;

[0034] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a vo...

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Abstract

The invention relates to a microbial remediation method for petroleum-contaminated soil. The microbial remediation method is characterized in that pseudomonas aeruginosa, achromobacter sp and a biosurfactant crude product are combined to repair petroleum-contaminated soil or degrade petroleum in oil sludge. The method has remarkable degradation effect on petroleum-contaminated soil or petroleum hydrocarbon in the oil sludge. According to a test, initial oil content of the petroleum-contaminated soil is 20000 mg / kg to 130000 mg / kg, and degradation efficiency can reach 75-91%. The method has the advantages of being low in treatment cost, high in treatment efficiency, free of secondary pollution and the like.

Description

technical field [0001] The invention belongs to a method for remediating oil-contaminated soil and treating oil sludge, and in particular relates to a microbial remediation method for oil-contaminated soil in arid areas in Northwest China. Background technique [0002] my country is a big country of oil production and consumption. Due to the damage and leakage of oil storage tanks, oil gathering and transportation pipelines and underground oil storage tanks of petroleum and petrochemical enterprises, as well as the occurrence of emergencies in the drilling and production process, the surrounding soil has been polluted to a certain extent. It is characterized by a large polluted area and complex sludge composition, including water, petroleum, heavy metals, clay, sand and gravel, etc., which is difficult to treat and highly toxic. At present, the domestic and foreign methods of sludge treatment include: solvent extraction method, hot detergent method, solidification method, py...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C02F11/02
CPCB09C1/10C02F11/02
Inventor 贾汉忠马健波王传义屈撑囤
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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