Achromobacter xyloseoxidans capable of degrading sulfide and its application

A colorless bacillus and sulfide technology, applied in the field of biological treatment of environmental pollutants, can solve environmental hazards, neurotoxicity and other problems, and achieve the effect of strong heat resistance

Active Publication Date: 2021-04-16
ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrogen sulfide gas will escape from the waste water. This is a colorless toxic gas with a strong smell of rotten eggs. It will not only cau

Method used

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  • Achromobacter xyloseoxidans capable of degrading sulfide and its application
  • Achromobacter xyloseoxidans capable of degrading sulfide and its application
  • Achromobacter xyloseoxidans capable of degrading sulfide and its application

Examples

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Effect test

Embodiment 1

[0025] Example 1: Isolation, purification and identification of Achromobacter xylosoxidans AX (Achromobacter xylosoxidans AX)

[0026] (1) Isolation and purification of Achromobacter xylosoxidans AX

[0027] Screening Achromobacter xylosoxidans strain AX from the wastewater treatment pond sludge of Zhejiang Satellite Energy Co., Ltd., the specific steps are as follows:

[0028] Take the sludge from the sewage of Zhejiang Satellite Energy Co., Ltd., let it stand for 24 hours, filter off the supernatant, take 10mL of the lower layer of sludge and inoculate it into a 250mL culture bottle containing 100mL of sulfide liquid selective medium, culture at 30°C, shake at 160rpm After 24 hours, the bacterial liquid was centrifuged, the bacterial cells were collected, and a certain concentration of the bacterial liquid was prepared with sterile water. The resulting bacterial solution was treated with Na 2 The S solid selection medium was separated and purified by streaking on multiple ...

Embodiment 2

[0041] Embodiment 2 Achromobacter xylosoxidans AX fermentation broth

[0042] (1) Slant culture: Achromobacter xylosoxidans AX was inoculated into the slant medium and cultured at 30°C for 7 days to obtain the slant of the bacteria; the composition of the slant medium was: peptone 10g L -1 , yeast powder 5g·L -1 , NaCl 10g·L -1 , the solvent is water, through 1mol L -1 Hydrochloric acid and 1mol L -1 Sodium hydroxide to adjust the pH value to 7.0, agar powder 15g L -1 .

[0043] (2) Seed culture: pick colonies from the slant of the bacteria and inoculate them into the seed medium, cultivate at 30°C for 1 day to obtain the seed liquid; the composition of the seed medium is: peptone 10g L -1 , yeast powder 5g·L -1 , NaCl 10g·L -1 , the solvent is water, through 1mol L -1 Hydrochloric acid and 1mol L -1 Sodium hydroxide was used to adjust the pH to 7.0.

[0044] (3) Fermentation culture: the seed liquid is inoculated to the fermentation medium with an inoculation amount...

Embodiment 3

[0045] Embodiment 3 Achromobacter xylosoxidans AX fermentation broth

[0046] (1) Slant culture: Achromobacter xylosoxidans AX was inoculated into the slant medium and cultured at 30°C for 7 days to obtain the slant of the bacteria; the concentration of the slant medium consisted of: peptone 10g L -1 , yeast powder 5g·L -1 , NaCl 10g·L -1 , the solvent is water, through 1mol L -1 Hydrochloric acid and 1mol L -1 Sodium hydroxide to adjust the pH value to 7.5, agar powder 18g L -1 .

[0047] (2) Seed culture: pick colonies from the slant of the bacteria and inoculate them into the seed medium, cultivate at 30°C for 1 day to obtain the seed liquid; the composition of the seed medium is: peptone 10g L -1 , yeast powder 5g·L -1 , NaCl 10g·L -1 , the solvent is water, through 1mol L -1 Hydrochloric acid and 1mol L -1 Sodium hydroxide was used to adjust the pH to 7.5.

[0048] (3) Fermentation culture: the seed liquid is inoculated to the fermentation medium with an inocula...

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Abstract

The invention discloses a new bacterial strain—Achromobacter xylosoxidans AX and its application for degrading sulfide. The Achromobacter xylosoxidans AX is preserved in the China Type Culture Collection Center, address: China, Wuhan, Wuhan University, deposit number: CCTCC NO: M 2019483, date of deposit: June 24, 2019; the invention provides a Achromobacter xylosoxidans AX with sulfide degradation performance, and the strain can detoxify the initial The concentration is 100mg·L ‑1 Na 2 The S degradation rate reached 94.4%. The discovery of this degrading bacteria is of great significance for the efficient purification of sulfide in industrial wastewater.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, in particular to an Achromobacter xylosoxidans AX capable of degrading sulfides and an application thereof. Background technique [0002] Sulfide in industrial and agricultural wastewater is an important cause of black and smelly water. This type of wastewater has a large amount of discharge, high pollution load, high toxicity, and will have a great impact on the normal operation of structures. Wastewater containing sulfides will harm aquatic organisms and cause potential corrosion if discharged directly into water bodies without treatment. Hydrogen sulfide gas will escape from the waste water. This is a colorless toxic gas with a strong smell of rotten eggs. It will not only cause human neurotoxicity, but also react with ozone in the atmosphere to form sulfuric acid, forming acid rain. greater harm to the environment. Therefore, it is particularly urgen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/10
CPCC02F3/34C02F2101/101C12N1/20C12N1/205C12R2001/025
Inventor 陈浚柳欢姚佳超朵延凯
Owner ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
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