Composition for controlling powdery mildew of astragalus and preparation method of composition
A powdery mildew and composition technology, applied in the field of plant protection agents, can solve the problems that resources cannot be reasonably used, the growth of astragalus is affected, and the root cure is difficult, and the preparation method is simple and controllable, strong resistance, and activity and durability are improved. Effect
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Embodiment 1
[0031] A preparation method for preventing and treating astragalus powdery mildew composition, comprising the following steps:
[0032] 1. Take active Monascus, add it to the culture medium, shake at constant temperature for 3 days, keep the temperature at 25°C, and get the initial culture medium; take half of the initial culture medium, add 3 times the amount of water, and add potassium dihydrogen phosphate , adjust the pH value to 7.5, add protease, control the temperature at 50°C, perform enzymatic hydrolysis for 3 hours, and centrifuge to obtain the filtrate (1). Put the remaining initial culture solution above into the strain culture tank, the mass ratio of the culture liquid in the strain culture tank to the initial culture liquid is 1:0.02, add the filtrate (1) obtained above, keep the temperature at 25°C, and culture in airtight for 15 days , that is, the optimized Monascus strain can be obtained.
[0033] 2. Inoculate the optimized Monascus seed solution into the fer...
Embodiment 2
[0037] A preparation method for preventing and treating astragalus powdery mildew composition, comprising the following steps:
[0038] 1. Take active Monascus, add it to the culture medium, shake at constant temperature for 2 days, keep the temperature at 20°C, and get the initial culture medium; take half of the initial culture medium, add 2 times the amount of water, and add potassium dihydrogen phosphate , adjust the pH value to 7, add protease, control the temperature at 40°C, perform enzymatic hydrolysis for 2 hours, and centrifuge to obtain the filtrate (1). Put the remaining initial culture solution above into the strain culture tank, the mass ratio of the culture liquid in the strain culture tank to the initial culture liquid is 1:0.01, add the filtrate (1) obtained above, keep the temperature at 20°C, and culture in airtight for 10 days , that is, the optimized Monascus strain can be obtained.
[0039] 2. Inoculate the optimized Monascus seed solution into the ferme...
Embodiment 3
[0043] A preparation method for preventing and treating astragalus powdery mildew composition, comprising the following steps:
[0044] 1. Take active Monascus, add it to the culture medium, shake at constant temperature for 4 days, keep the temperature at 30°C, and get the initial culture medium; take half of the initial culture medium, add 4 times the amount of water, and add potassium dihydrogen phosphate , adjust the pH value to 8, add protease, control the temperature at 60°C, perform enzymatic hydrolysis for 4 hours, and centrifuge to obtain the filtrate (1). Put the remaining initial culture solution above into the strain culture tank, the mass ratio of the culture liquid in the strain culture tank to the initial culture liquid is 1:0.03, add the filtrate (1) obtained above, keep the temperature at 30°C, and culture in airtight for 20 days , that is, the optimized Monascus strain can be obtained.
[0045] 2. Inoculate the optimized Monascus seed solution into the ferme...
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