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Bacterial population sensing inhibitor and screening method thereof

A technology of quorum sensing and screening methods, applied in biochemical equipment and methods, applications, food science, etc., can solve problems such as food pollution, food poisoning, and complex microbial metabolites, and achieve good fresh-keeping effects and rich seaweed resources.

Inactive Publication Date: 2012-01-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of quorum sensing inhibitors derived from microorganisms in food has limitations. The microbial metabolites are complex and often produce harmful metabolites, which can pollute food or cause food poisoning.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Spirulina platensis was freeze-dried, pulverized, passed through a 40-mesh sieve, 100 g of powder was added, 1000 mL of 75% ethanol was added, ultrasonically extracted for 30 min, the residue was removed by filtration, the residue was extracted twice, and the combined filtrate was evaporated to dryness by a rotary evaporator for three times (vacuum 0.1kPa, temperature 30°C), concentrate the extract (13.25g), add the same volume of petroleum ether and distilled water for extraction, discard the petroleum ether phase, then extract the water phase with ethyl acetate, discard the ethyl acetate, and use the water phase for Evaporate to dryness with a rotary evaporator, and use distilled water to make a seaweed extract with a mass volume concentration of 0.125%-1%. The extract is sterilized by filtering through a 0.22 μm filter membrane, and passed through the bacterial biosensor Violaceum CV026 and hexanoyl homoserine lactone. Detection is carried out, and the detection resul...

Embodiment 2

[0022] Sargassum sargassum was freeze-dried, crushed, passed through a 40-mesh sieve, 100g of powder was taken, added 1000mL of 80% ethanol, ultrasonically extracted for 30min, and the residue was removed by filtration. The product was concentrated (3.82g), extracted successively with petroleum ether and ethyl acetate, the water phase was taken, and the water phase was evaporated to dryness with a rotary evaporator, and distilled water was used to prepare a seaweed extract with a mass volume concentration of 0.1g / mL-1g / mL. After sterilization, the extract was detected by bacterial biosensor Violet Bacillus CV026 and hexanoyl homoserine lactone. The test result of the extract was positive, and it was determined to be a bacterial quorum sensing inhibitor-Sargassum schistophora inhibitor.

[0023] In order to verify the effectiveness of the method of the present invention, fresh shrimps are soaked in the inhibitor aqueous solution of the present embodiment with a concentration of ...

Embodiment 3

[0025] Undaria pinnatifida was freeze-dried, crushed, passed through a 40-mesh sieve, 100g of powder was taken, 1000mL of 85% ethanol was added, and ultrasonically extracted for 30 minutes to obtain 15.32g of seaweed extract, which was extracted with petroleum ether and ethyl acetate successively, and the water phase and the water phase Evaporate to dryness with a rotary evaporator, and prepare a seaweed extract with a mass volume concentration of 0.01 g / mL-1 g / mL with distilled water. After sterilization, it was detected by the bacterial biosensor Violaceum violaceum CV026 and hexanoyl homoserine lactone. The test result of the extract was positive, and it was determined to be a bacterial quorum sensing inhibitor-wakame inhibitor.

[0026] In order to verify the effectiveness of the method of the present invention, fresh shrimps were soaked in the inhibitor aqueous solution of the present embodiment with a concentration of 0.01g / mL-1g / mL, taken out and preserved as an experime...

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Abstract

The invention relates to a bacterial population sensing inhibitor and a screening method thereof. The screening method is characterized by comprising the following steps of: drying algae and smashing; adding an ethanol solution for extracting; and detecting an extracting solution with bacterium biosensor chromobacterium CV026 and caproyl homoserine lactone, wherein the extracting solution is the bacterial population sensing inhibitor if a detection result is positive. The inhibitor screening method is quick and effective, and the bacterial population sensing inhibitor can be efficiently screened from marine algae. The bacterial population sensing inhibitor screened with the method can be applied to preservation of fresh foods or products, and is natural and nontoxic and has a better preservation effect in comparison to the conventional chemical synthesis inhibitor. The screening method is suitable for industrial production. The yield of the prepared inhibitor can well meet practical application.

Description

technical field [0001] The invention belongs to the technical field of food preservation, and in particular relates to a bacterial quorum sensing inhibitor and a screening method thereof. Background technique [0002] Quorum Sensing (QS for short) of bacteria is a sensing phenomenon (Sensing) that depends on the number of bacteria (Quorum) reaching a certain density. When the number of bacteria in a specific environment increases, the expression of bacterial target genes will be activated to exhibit new behavioral characteristics, such as bioluminescence, plasmid transfer, production of virulence factors, spore germination, and biofilm formation. The spoilage process of food is regulated by the QS system of spoilage bacteria. Using quorum sensing signal molecules as new targets to regulate the process of food spoilage provides a new strategy for food storage and preservation. [0003] Affecting the QS system by interfering with the binding of signaling molecules and recepto...

Claims

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Application Information

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IPC IPC(8): A23L3/3472C12Q1/02
Inventor 曾名湧曾惠朱素芹刘尊英董士远赵元晖
Owner OCEAN UNIV OF CHINA
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