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A kind of carbendazim detection method based on fluorescent probe

A technology of fluorescent probes and detection methods, applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of complex model building process, and achieve the effect of low cost, high specificity and simple method

Active Publication Date: 2018-02-27
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method can realize the simultaneous quantitative detection of two target pesticides in vegetables, the process of building the model and collecting the three-dimensional excitation-emission matrix fluorescence spectra of each sample is still relatively complicated.

Method used

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  • A kind of carbendazim detection method based on fluorescent probe
  • A kind of carbendazim detection method based on fluorescent probe

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The detection of carbendazim content in embodiment 1 tomato

[0034] Preparation of tomato samples

[0035] Weigh 10 g of tomato, add 0.1 g of sodium carbonate and 20 mL of ethyl acetate, vortex and mix for 1 min, then sonicate for 25 min, and after centrifugation, take 10 mL of the supernatant and dry it with weak nitrogen in a water bath at 45 °C. Dissolving residues for purification;

[0036] SPE cartridges (OasisMCX, 60mg, 3cc) were activated before use with 2mL methanol and 2mL 0.15mol / L ammonia water sequentially; the above tomato samples dissolved in hydrochloric acid were loaded onto the SPE cartridges, and 1mL 0.15 mol / L ammonia water, 1mL ammonia water (0.15mol / L)-methanol solution, 1mL 0.1mol / L hydrochloric acid, 1mL methanol to elute the column, discard the elution effluent, and control the flow rate of the entire elutriation process within 3mL / min ; Finally, the column was eluted with 2mL ammonia water (0.3mol / L)-methanol solution, and the eluate was co...

Embodiment 2

[0046] The detection of carbendazim content in embodiment 2 citrus

[0047] Preparation of citrus samples

[0048] Weigh 10 g of citrus as it is, and use the same method as in Example 1 to pretreat and purify the citrus as it is, to prepare a citrus sample.

[0049] Determination of carbendazim content in citrus samples

[0050] (1) Weigh 10mg of 9,10-phenanthrenequinone and dissolve it with a small amount of DMF, dilute to volume in a 25mL brown volumetric flask, and prepare 400ppm solution A for later use;

[0051] (2) Dissolve 200mg of sodium hydroxide with deionized water, make it constant in a 10mL volumetric flask, and prepare 0.5mol / L aqueous sodium hydroxide solution B for subsequent use;

[0052] (3) Prepare carbendazim standard solution: prepare 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm, 25ppm and 100ppm carbendazim aqueous solutions with deionized water as standard solutions. Add 0.5mL solution A, 0.2mL solution B and 1.0mL carbendazim standard to a 2.0mL centrifuge...

Embodiment 3

[0057] The detection of carbendazim content in embodiment 3 apples

[0058] Preparation of apple samples

[0059] Weigh 10 g of apples as they are, and use the same method as in Example 1 to pretreat and purify the apples as they are, to obtain an apple sample.

[0060] Determination of carbendazim content in apple samples

[0061] (1) Weigh 15 mg of 9,10-phenanthrenequinone and dissolve it with a small amount of DMSO, dilute to volume in a 25 mL brown volumetric flask, and prepare 600 ppm solution A for later use;

[0062] (2) Dissolve 600mg of sodium hydroxide with deionized water, make it constant in a 10mL volumetric flask, and prepare 1.5mol / L sodium hydroxide aqueous solution B for subsequent use;

[0063] (3) Prepare carbendazim standard solution: prepare 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm, 25ppm and 100ppm carbendazim aqueous solutions with deionized water as standard solutions. Add 0.5mL solution A, 0.1mL solution B and 1.0mL carbendazim standard to a 2.0mL cen...

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Abstract

The invention provides a carbendazim detection method based on a fluorescent probe, comprising the following steps: dissolving the fluorescent probe with an organic solvent to prepare a solution A; preparing an alkali solution B; preparing a carbendazim standard solution, adding Solution A and solution B, detect the fluorescence signal intensity, draw the standard curve of the relationship between the fluorescence intensity and the concentration of the carbendazim standard solution; prepare the sample solution to be tested, add solution A and solution B to it, detect the fluorescence signal intensity, according to the obtained The content of carbendazim in the samples was obtained from the standard curve. The present invention generates a fluorescent signal through the specific reaction between the fluorescent probe and the carbendazim, and determines the content of the carbendazim in the sample according to the relationship between the fluorescent signal intensity and the concentration by detecting the intensity of the fluorescent signal, the method is simple, the cost is low, and the The detection of carbendazim content under alkaline conditions has the characteristics of high specificity, high sensitivity, accuracy and reliability, and can be used for rapid detection of carbendazim content in fruits and vegetables.

Description

technical field [0001] The invention belongs to the technical field of food safety analysis, relates to a carbendazim detection method, in particular to a fluorescent probe-based carbendazim detection method. Background technique [0002] Carbendazim, a benzimidazole drug, is also the main metabolite of benomyl in mammals and its degradation product in the environment. As a broad-spectrum and efficient fungicide, carbendazim is widely used in the prevention and treatment of powdery mildew and downy mildew of various agricultural products such as vegetables and fruits. It is low-toxic to humans and animals, but if it is ingested for a long time, it will accumulate in the body , thus causing chronic or acute toxicity and affecting human health. It can cause excitement, convulsions, trance, nausea and vomiting, dizziness, headache, chest tightness, upper abdominal tenderness and other poisoning symptoms through the esophagus. [0003] At present, the existing carbendazim detec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 王淼李敏敏陈卫华王凤忠朱玉龙贺妍范蓓尚玉婷孙玉凤卢嘉
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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