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The invention relates to the technical field of pathogen detection, and discloses a kit for rapid detection and simultaneous identification of Zika virus and Japanese encephalitis virus by a one-tube method and an application thereof. The kit comprises an enzyme dry powder group, an RPA dual amplification system and a dual-target nucleic acid test strip, wherein the RPA dual amplification system comprises a ZIKV-F / ZIKV-R primer pair, a JEV-F / JEV-R primer pair, a ZIKV-Pb probe and a JEV-Pb probe; the ZIKV-F / ZIKV-R primer pair comprises SEQ ID NO.1 and SEQ ID NO.2; the JEV-F / JEV-R primer pair comprises SEQ ID NO.4 and SEQ ID NO.5; the ZIKV-Pb probe comprises SEQ ID NO.3; and the JEV-Pb probe comprises SEQ ID NO.6. This scheme designs specific primer pairs and probes for the genomic RNA of the two viruses, and modifies the primers / probes of the two viruses with different positioning antigen molecules and color antigen molecules, so as to facilitate the rapid and simultaneous detection of whether the sample contains one or both of the two viruses, thereby achieving simultaneous detection of the two viruses.

This invention discloses a bio-based hydrophobic monomer, a triple shape memory hydrogel, and their applications. The preparation method of the bio-based hydrophobic monomer includes the following steps: In an organic solvent, dehydrorosinyl chloride and N-(2-hydroxyethyl)-2-methyl-2-acrylamide are mixed and reacted to obtain the bio-based hydrophobic monomer. This invention prepares a novel bio-based hydrophobic monomer and applies it to hydrogel preparation, obtaining a hydrogel with high strength and triple shape memory function, which can be used for rapid and visual detection of Fe in water. 3+ Concentration and fabrication of large strain sensors.