75results about How to "Quick Visual Inspection" patented technology

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and/or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention discloses a ratio fluorescent probe, a fluorescent paper chip and a detection method for detecting mercury ions, the ratio fluorescent probe comprises a background probe and a reaction probe, the background probe is composed of nitrogen-doped blue carbon quantum dots, the reaction probe is composed of red gold nanoclusters, the ratiometric fluorescent probe is formed by mixing red gold nanoclusters and nitrogen-doped blue carbon quantum dots, after mercury ions are added into the ratiometric fluorescent probe, the red fluorescence of the gold nanoclusters is gradually quenched, and the fluorescence of the nitrogen-doped blue carbon quantum dots is kept unchanged and is used as a reference signal; and therefore, the On-Off type fluorescent probe is formed. The two fluorescent materials of the ratiometric fluorescent probe are easy to prepare, and the ratiometric fluorescent probe has the characteristics of low toxicity, high fluorescence intensity, good biocompatibility and the like, has a wider color change range, and is more beneficial to detection of mercury ions. When the probe is fixed on a paper chip sensor, rapid visual detection of mercury ions can be realized.

Methods and a system for an electronic device to allow for indicating a size or scale of a rendering in a three dimensional view. A virtual scene is displayed on a display with a work product that is rendered, such as a document or packaging request having a job ticket. The rendering is provided with a common object that is a three dimensional virtual object that relates size to the document or packaging for approval in the proofing process. A quick visual cue is therefore provided to visually convey a realistic scale in proportion to the product rendered three dimensionally.

The invention discloses a Cpf1 kit for rapid detection of hereditary deafness pathogenic gene SLC26A4 mutation and a detection method thereof. The Cpf1 kit comprises a Cpf1 detection system. The Cpf1detection system comprises: a specific crRNA for SLC26A4, a Cpf1 protein and a single-stranded DNA reporting system. The specific crRNA is any one or more designed and synthesized for mutation sites.The single-stranded DNA reporting system comprises an ssDNA FQ reporter for fluorescence detection by an enzyme-labeled instrument and/or an ssDNA DB reporter for immune colloidal gold strip detection. By detecting SLC26A4 gene mutation sites with the Cpf1 for the first time, the kit of the invention has the advantages of high sensitivity, strong specificity, low time consumption, no dependence onlarge-scale experimental equipment and the like.

The invention discloses an insulin pen storage medical vehicle, which comprises a vehicle frame main body, a top-layer storage platform and rollers, wherein the top-layer storage platform comprises a top platform surface, and a first accommodation cabinet and a second accommodation cabinet, which are arranged below the top platform surface; the top platform is provided with an open groove communicated with the first accommodation cabinet; a circle of concave platform is arranged at a groove opening of the open groove in a downward sinking manner, and is detachably covered with a cover plate; multiple insulin pen placement plates are arranged in the first accommodation cabinet; multiple insulin pen clamping frames are arranged on the end face of at least one side of each insulin pen placement plate; a tag used for indicating patient information is adhered to each insulin pen clamping frame. The insulin pen storage medical vehicle has the effects that the insulin pen at a corresponding position can be taken to inject a corresponding patient according to the patient information on the tag A when the cover plate is removed, the insulin pens are placed in order, and can be rapidly taken, and medical staff can also push the insulin pen storage medical vehicle to each sickroom for centralized injection of patients, so that the insulin pen storage medical vehicle is high in practicability, and has good using effects.

The invention provides a fast visual detection method of organophosphorus type pesticide. Firstly, ZnTPyP is subjected to hydrothermal self-assembly by a cationic surface active agent for preparing nanometer porphyrin; then, the nanometer porphyrin is used for the fast visual detection on organophosphorus. On the basis of biomimetic peroxidase type activity of the nanometer porphyrin, 3,3',5,5'-tetramethyl benzidine is catalyzed and oxidized; the characteristic absorption in a 652nm position of the ultraviolet-visible spectrum occurs; colorless to blue chromogenic reaction is generated; specific reaction of the organophosphorus type pesticide and the nanometer porphyrin is utilized; the catalytic activity of the nanometer porphyrin is reduced; the generation of blue oxidation product oxTMBis reduced; the reduction of the absorbancy in the 652nm position of the ultraviolet-visible spectrum is shown; the reduction degree and the concentration of the organophosphorus type pesticide are in positive correlation relationship; the detection of the organophosphorus type pesticide is realized; the operation of the detection method is simple and convenient; the reagent toxicity is small; the cost is low; the sensitivity is high; the stability is high; the applicability is high.

The invention provides a colorimetric sensor for enhancing MOFs enzyme-like activity based on an aptamer as well as a preparation method and application of the colorimetric sensor, and belongs to the technical field of sensor preparation and fruit and vegetable quality detection. According to the present invention, the colorimetric sensor is constructed by using the metal organic framework material (MOFs) with peroxidase-like activity to catalyze and oxidize 3, 3', 5, 5'-Tetramethylbenzidine (TMB), and the colorimetric sensor can achieve the visual rapid monitoring of the pesticide residues in the fruits and the vegetables based on the enhancement effect of the aptamer on the catalytic activity of the MOFs and the specific recognition between the aptamer and the pesticide.

The invention belongs to the field of environmental monitoring, and discloses an iron-based metal organic framework material Fe-MOFs and a synthesis method thereof. 3,5-dicarboxyphenylboronic acid is used as a ligand to be assembled with metal iron ions, and the iron-based metal organic framework material (Fe-MOFs) with boric acid functionalization is obtained under the action of a regulator. A research result shows that the Fe-MOFs nano material shows excellent peroxidase mimetic property. In the presence of hydrogen peroxide, the Fe-MOFs can catalytically oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to show a blue color. Meanwhile, when fluorine ions exist in the system, the peroxidase property of the Fe-MOFs is inhibited based on the specific recognition of boric acid on the fluorine ions and the strong interaction between metal ions and the fluorine ions. On the basis, the invention constructs a novel method for colorimetric detection of fluorine ions. The method has relatively high selectivity and sensitivity on detection of the fluorine ions, and has great application potential on visual detection of the fluorine ions in environmental monitoring.

The invention discloses a novel visual pathogen nucleic acid rapid detection chip which comprises a substrate and a flow path sheet. The substrate and the flow path sheet are sealed into one whole body; the substrate is composed of a bottom plate, a USB interface, a heating resistor and a thermosensitive element; the flow path sheet is a communicating pipeline structure composed of a buffer solution hole, a sample adding hole, a reaction region, a detection region and a waste liquid vessel; the detection region consists of a chromatography test paper card; a long-chain nucleic acid probe I, a long-chain gold-labeled nucleic acid probe II, a short-chain gold-labeled nucleic acid probe III and a short-chain gold-labeled nucleic acid probe IV are fixed on the chromatography test paper card; and the USB interface can be connected with a mobile phone charger and the like. According to the novel visual pathogen nucleic acid rapid detection chip, rapid visual detection of new coronavirus pathogens in a saliva sample is implemented, the saliva sample is taken, a lysis solution is added for lysis, RNA is extracted through a paramagnetic particle method, fluid is controlled through the liquid driving force of a buffer solution, the one-step reaction is performed, and visual detection of results is implemented within one hour without any instrument.

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for zinc ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system, (3)a HCR system, and (4) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of thetemplate can be realized in the presence of zinc ions, and an amplification product is allowed to form a G-quadruplex in a proper environment by triggering a HCR reaction; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of zinc ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to zinc ions, and is more objective and accurate in detection results.

The invention relates to the technical field of plant pathogen detection, in particular to a loop-mediated isothermal amp lification (LAMP) primer for rapidly detecting meloidogyne javanica, application of the LAMP primer and a detection method of the LAMP primer. The LAMP primer comprises a forward outer primer Mj-F3, a reverse outer primer Mj-B3, a forward inner primer Mj-FIP and a reverse innerprimer Mj-BIP; and primer nucleotide sequences are sequentially shown as SEQ ID NO.2-5. A LAMP detection method is built based on the LAMP primer, can be applied to rapid detection and identificationof the meloidogyne javanica in nematode samples, plant root tissue samples and soil samples and has the advantages of high specificity, high sensitivity, simple instrument equipment, simple operation, fastness and high efficiency, visual determination of a result and the like, and high popularization and application value is achieved in aspects such as field rapid detection of the meloidogyne javanica and early diagnosis and guide scientific drug use of the meloidogyne javanica.

The invention provides a visual detection method of magnesium and zinc cutting type functional nucleic acids for universal partition and ultra-fast amplification. The visual detection method providedby the invention can perform ultra-fast amplification on templates the presence of magnesium and zinc ions by ingeniously designing primers, the templates and probes, enables amplification products toform a G-quadruplex in a suitable environment, further uses peroxidase-like activity of the G-quadruplex for color development, solves a problem that traditional PCR products are hard to be detectedvisually, and realizes fast and visual detection of magnesium and zinc ions. Moreover, the visual detection method provided by the invention has the characteristics of high specificity and high sensitivity to magnesium and zinc ions, and more objective and accurate detection results.

The invention discloses a visual detection method for aflatoxin B1. The visual detection method comprises the following steps: respectively linking sulfhydrylated aflatoxin B1 aptamer DNA1 and a complementary chain DNA2 of a sulfhydrylated aptamer to 13nm+/-2nm nanogold, so to obtain an aptamer-nanogold probe and a complementary chain-nanogold probe, and carrying out detection by virtue of a competition method, wherein a drone is bonded with the aptamer when a to-be-detected object contains the drone, the aptamer-nanogold probe and the complementary chain-nanogold probe are in a free state, nanogold is easily gathered under a high salt concentration condition, the color of the nanogold is changed from red to blue, when the to-be-detected object does not contain the drone, the nanogold is not gathered under the high salt concentration condition, and the color of the nanogold is still red and is gradually changed from red to blue along the increase of the concentration of the drone; anddetecting an ultraviolet-visible spectrum of the solution at 200nm-800nm, so as to realize the quantitative detection of aflatoxin B1 in the to-be-detected object.

The invention relates to a rapid detection kit for pathogenic bacteria of phoma stem canker and a using method of the rapid detection kit. The rapid detection kit comprises a rapid detection method and a kit for leptosphaeria maculans and leptosphaeria biglobosa. The detection method for the pathogenic bacteria comprises specific amplification of target nucleic acid molecules and specific detection based on specific guide RNA, Cas12a and a nucleic acid probe. By using the method provided by the invention, whether the leptosphaeria maculans and the leptosphaeria biglobosa are contained in rape plant tissues can be rapidly detected. In combination with a nucleic acid isothermal amplification technology, the sensitivity of the detection method can be greatly improved, and the detection method can be used in fields and quarantine sites. The rapid detection kit for the pathogenic bacteria of the phoma stem canker is used for detecting the leptosphaeria maculans or leptosphaeria biglobosa, has the advantages of high sensitivity, good specificity, short consumed time, no dependence on large experimental equipment and the like, and is conveniently used for field disease investigation as well as rapid detection and identification diagnosis of the pathogenic bacteria of the phoma stem canker in primary laboratories.

The invention relates to technical field of biology and in particular to discloses a LAMP (Loop-Mediated Isothermal Amplification) primer group, a kit and a detection method for detecting lotus rot pathogen. A group of primer group F3/B3 and FIP/BIP is designed by aiming at a lotus rot pathogen translation elongation factor (TEF-1 alpha) gene sequence, the primer group or the kit containing the primer group is used for detecting the lotus rot pathogen by virtue of LAMP. The LAMP primer group and the kit have the advantages of low cost, easiness in operation, simplicity, rapidness, visual detection result, judgment capability based on naked eyes, high sensitivity, strong specificity and the like, are applicable to lotus (lotus root) quarantine for introducing seeds, seedling and breeding lotus root quality test, lotus rot pathogen field early diagnosis and the like in basic departments, and have wide and practical application value.

The invention relates to a boron dipyrromethene fluorescent probe and a preparation method and application thereof in viscosity detection, and in particular relates to a method for measuring intracellular viscosity by using a fluorescent molecular probe based on a boron dipyrromethene structure. The fluorescence of the fluorescent probe is very weak in a low-viscosity system, the fluorescence of the fluorescent probe is enhanced in a high-viscosity solvent system such as glycerol, and meanwhile, a certain degree of emission spectrum red shift phenomenon is accompanied; therefore, the probe canbe used for detecting the viscosity in different solvent systems; and meanwhile, the probe has good biocompatibility and low toxicity, can be used for detecting viscosity change in cells, and has specific positioning performance on mitochondria.

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system and (3) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can berealized in the presence of chromium ions, and an amplification product is allowed to form a G-quadruplex in a proper environment; and color development is performed by utilizing the peroxidase-likeactivity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of chromium ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to chromium ions, and is more objective and accurate in detection results.