4results about How to "Quick Visual Inspection" patented technology

The invention relates to a color developing agent embedded CuMOFs nano-enzyme paper-based sensor and a preparation method and application thereof.The preparation method comprises the steps that water-soluble TMB-NPs is prepared from water-insoluble typical peroxide mimic enzyme color developing substrates TMB and BSA, the water-soluble TMB-NPs is embedded in CuMOFs in situ through a coprecipitation method, and CuMOFs-TMB-NPs is obtained; the synthesis method is simple and convenient, and the synthesized material has excellent enzyme-like activity and stability. Then, qualitative filter paper is used as a paper-based material, CuMOFs-TMB-NPs is uniformly coated on the paper-based material, and the paper-based sensor of H2O2 and AMP is constructed based on the paper-based material. The CuMOFs disintegrates in an acid environment to release TMB-NPs and a large amount of Cu < 2 + >, the TMB-NPs serves as an enzyme catalysis substrate, and the Cu < 2 + > serves as a catalyst to participate in subsequent reaction. The paper-based sensor can be used for visual detection of H2O2 and AMP in a water body.

The invention relates to the technical field of pathogen detection, and discloses a kit for rapid detection and simultaneous identification of Zika virus and Japanese encephalitis virus by a one-tube method and an application thereof. The kit comprises an enzyme dry powder group, an RPA dual amplification system and a dual-target nucleic acid test strip, wherein the RPA dual amplification system comprises a ZIKV-F / ZIKV-R primer pair, a JEV-F / JEV-R primer pair, a ZIKV-Pb probe and a JEV-Pb probe; the ZIKV-F / ZIKV-R primer pair comprises SEQ ID NO.1 and SEQ ID NO.2; the JEV-F / JEV-R primer pair comprises SEQ ID NO.4 and SEQ ID NO.5; the ZIKV-Pb probe comprises SEQ ID NO.3; and the JEV-Pb probe comprises SEQ ID NO.6. This scheme designs specific primer pairs and probes for the genomic RNA of the two viruses, and modifies the primers / probes of the two viruses with different positioning antigen molecules and color antigen molecules, so as to facilitate the rapid and simultaneous detection of whether the sample contains one or both of the two viruses, thereby achieving simultaneous detection of the two viruses.

This invention discloses a bio-based hydrophobic monomer, a triple shape memory hydrogel, and their applications. The preparation method of the bio-based hydrophobic monomer includes the following steps: In an organic solvent, dehydrorosinyl chloride and N-(2-hydroxyethyl)-2-methyl-2-acrylamide are mixed and reacted to obtain the bio-based hydrophobic monomer. This invention prepares a novel bio-based hydrophobic monomer and applies it to hydrogel preparation, obtaining a hydrogel with high strength and triple shape memory function, which can be used for rapid and visual detection of Fe in water. 3+ Concentration and fabrication of large strain sensors.

Magnetic Fe3O4 is taken as an imprinting carrier, H5N1 and H7N9 are taken as templates, red and green quantum dots are respectively introduced as fluorescence signal output, a molecular imprinting cladding strategy is adopted to reduce non-specific adsorption, two imprinting polymers H5N1-cMIPs and H7N9-cMIPs are prepared, and viruses can be quantitatively detected through fluorescence spectrum. Meanwhile, target virus aptamers are respectively modified to the surface of the ZIF-8, so that signal amplification probes of the ZIF-8 (at) CPNs-H5N1Apt and the ZIF-8 (at) CPNs-H7N9Apt are obtained. The H5N1 and the H7N9 are respectively captured by the H5N1-cMIPs and the H7N9-cMIPs, and then the H5N1 and the H7N9 are combined with the ZIF-8 (at) CPNs-H5N1Apt and the ZIF-8 (at) CPNs-H7N9Apt through aptamer specific recognition, so that a sandwich structure is formed. Under an acidic condition, the ZIF-8 can be cracked to release copper ions and hydrogen peroxide, and the oxidized TMB achieves visual detection. The method has the advantages that the H5N1 and the H7N9 can be detected in a semi-quantitative manner through visual observation, the method is simple in operation, portable and rapid in detection, POC diagnosis can be carried out in a resource-deficient environment by the aid of the simplified equipment, and the method has important practical application value.