The invention discloses a chemiluminescence enzyme immunoassay detection reagent kit for nitrofurantion, which comprises a kit body, an ELIAS plate arranged in the kit body and a reagent in the kit body, wherein each hole of the ELIAS plate is enveloped with an envelope antigen, and the envelope antigen is produced by coupling a metabolite 1-aminohydantoin (AHD) of the nitrofurantion and carrier protein; and the reagent comprises a polyclonal antibody of the nitrofurantion, a goat anti-rabbit antibody labeled by an ELIAS secondary antibody (horseradish peroxidase), a nitrofurantion series standard solution, a condensed phosphate buffer liquid, a concentrated cleaning solution, and a luminescent solution. The reagent kit has the characteristics of high sensitivity, good repetitiveness, simplicity, convenience, quickness, and accuracy; compared with the prior colorimetric ELISA method, the sensitivity can be improved by one order of magnitude; and the reagent kit is expected to play an important role in residue detection of the nitrofurantion of water for livestock raising, feedstuff, and animal derived food, such as milk samples, animal tissue samples, and urine samples.