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Preparation method of water-soluble probe identifying Fe<3+> and application thereof

A water-soluble, probe technology, used in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., can solve the problems of expensive instruments, long time, high cost, etc., to reduce production and preparation costs, shorten the preparation process time, and can Actionable effect

Inactive Publication Date: 2017-05-24
SUZHOU CHIEN SHIUNG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have high sensitivity, they are not the best method for detecting samples because they often require expensive instruments, complicated processing procedures, high cost, and long-term consumption.

Method used

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  • Preparation method of water-soluble probe identifying Fe&lt;3+&gt; and application thereof
  • Preparation method of water-soluble probe identifying Fe&lt;3+&gt; and application thereof
  • Preparation method of water-soluble probe identifying Fe&lt;3+&gt; and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment relates to the preparation of water-soluble probes:

[0035] (1) Synthesis of 7-diethylaminocoumarin (1)

[0036] Add (1.93g, 10mmol) 4-diethylamino salicylaldehyde, (3.2g, 20mmol) diethyl malonate and 1mL piperidine into 30mL ethanol, stir at reflux at 90°C for 6h, and distill off the solvent under reduced pressure . Then 20 mL of glacial acetic acid and 20 mL of concentrated hydrochloric acid were added to the mixture, stirred at reflux for 6 h, cooled to room temperature, and then poured into 100 mL of ice water. 40% NaOH solution was added dropwise to adjust the pH value of the solution to 5, and stirred for 30 min to obtain a white precipitate. Filter, wash with water, dry, and recrystallize from toluene to obtain the synthesis of 7-diethylaminocoumarin (1.74 g, 8 mmol), with a yield of 80.1%.

[0037] (2) Synthesis of 7-diethylamino-3-formaldehyde coumarin (L)

[0038] N 2 Under protection, add 2mL POCl dropwise to the freshly distilled 2mL DM...

Embodiment 2

[0040] This example involves the identification of Fe 3+ Applications of Water Soluble Probes

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Abstract

The invention discloses a preparation method of a water-soluble probe identifying Fe<3+>. The preparation method includes the steps that 1, 4-diethyl salicylaldehyde, diethyl malonate and pryridine are dissolved in ethyl alcohol, reflux and stirring are performed for 6-8 hours, and reduced pressure distillation is performed to remove the ethyl alcohol; then, glacial acetic acid and concentrated hydrochloric acid are added, reflux and stirring are performed for 6-8 hours, cooling is performed to reach room temperature, then ice water is added, a pH value is regulated to be 4-5, white precipitate is obtained, filtration is performed, washing is performed with water, and then methylbenzene recrystallization is performed to obtain 7-diethylamino coumarin; 2, under N2 protection, POCl3 is dropwise added to DMF, stirring is performed at the temperature of 20-50 DEG C for 30 minutes, then a 7-diethylamino coumarin-DMF solution is dropwise added to obtain a red suspension; stirring is performed at the temperature of 60 DEG C for 12-14 hours, then the suspension is poured into ice water to obtain a mixed solution; a 20% NaOH solution is added to the mixed solution to get a crude product; the crude products are recrystallized by anhydrous ethanol to obtain the probe.

Description

technical field [0001] This application relates to a kind of identification Fe 3+ The synthesis of the water-soluble probe belongs to the technical field of organic synthesis; and because the application relates to the biological application of the water-soluble probe in cells, it also belongs to the field of analysis and detection of metal content in organisms. Background technique [0002] Various metals exist in nature, including alkaline earth metals, alkali metals, transition metals and heavy metal elements, and these metals are closely related to the environmental, biological, medical and chemical disciplines. Among various trace elements, iron is the most abundant and important transition metal, which plays an important role in the life of organisms. Iron is an indispensable element in human physiological activities. In the blood, iron plays an important role in oxygen transport, protein transfer, synthesis of DNA and RNA, and maintenance of intracellular osmotic pre...

Claims

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Application Information

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IPC IPC(8): C07D311/16C09K11/06G01N21/64
CPCC07D311/16C09K11/06C09K2211/1088G01N21/6486G01N2021/6432
Inventor 解雪乔
Owner SUZHOU CHIEN SHIUNG INST OF TECH
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