7 results about "Imperatorin" patented technology

This invention belongs to the field of traditional Chinese medicine treatment, specifically relating to an anti-cold and detoxifying capsule pharmaceutical composition, its preparation method, and its application. The composition comprises the following components in parts by weight: 25-35 parts Angelica dahurica, 40-50 parts Scutellaria baicalensis, 2-5 parts flavonoids, 0.5-2 parts Imperatorin, 1-5 parts Artemisia capillaris, 1-5 parts phloroglucinol compounds, and 0.1-0.5 parts organic acids. The component-based composition provided by this invention has a good effect in preventing and treating wind-heat colds, and the components exhibit good synergistic effects.

This invention discloses a topical traditional Chinese medicine composition for relieving pain and its preparation method, belonging to the field of traditional Chinese medicine preparation technology. The topical traditional Chinese medicine composition for relieving pain is made from the following raw materials in parts by weight: Angelica sinensis 5-25 parts, Ligusticum striatum 5-20 parts, Lycopodium clavatum 10-35 parts, Artemisia argyi 5-25 parts, artificial Phellodendron chinense 3-18 parts, Clematis chinensis 10-40 parts, and Angelica dahurica 5-20 parts. This invention uses steam distillation to extract volatile oils and a dual-solvent gradient extraction method with the residue, which not only fully extracts the volatile fat-soluble components from Angelica sinensis, Ligusticum striatum, and Angelica dahurica, but also efficiently extracts the water-soluble components from artificial Phellodendron chinense, Lycopodium clavatum, and other medicinal materials. Experimental results show that the extraction rates of ferulic acid, ligustilide, imperatorin, and berberine hydrochloride in Example 1 of this invention reached 89.23%, 92.56%, 90.12%, and 93.45%, respectively, which are 110.7%, 223.1%, 75.8%, and 23.6% higher than those of conventional water decoction process, thus improving the utilization rate of raw materials.

The application discloses a method for constructing a characteristic map of a traditional Chinese medicine and the characteristic map, wherein the method adopts an HPLC method to determine the content of an effective component, imperatorin, in Zhikang capsules, and constructs a high performance liquid chromatography characteristic map of the Zhikang capsules; 10 characteristic peaks are determined as the characteristic map, which are respectively from dragon's blood, rhubarb and Bai Zhi; the components from the dragon's blood are dracorhodin B (peak 2) and pterostilbene (peak 4); the components from the rhubarb are peak 1 (aloe-emodin), peak 3, peak 6 (emodin), peak 9 and peak 10; and the components from the Bai Zhi are imperatorin (peak 5), peak 7 and peak 8; the method covers polyphenols, anthraquinones and furanocoumarins and other main components, and constructs a quality evaluation system of the Zhikang capsules; the method has good specificity, precision, repeatability and solution stability, and can be used for standardizing the production of the Zhikang capsules and guaranteeing the uniformity and stability of the internal quality of products to provide comprehensive quality control.

The application discloses a kind of shentong zhuyu decoction fingerprint construction method and application, belong to the technical field of drug detection.The application is detected by high performance liquid chromatography to several batches of shentong zhuyu decoction by the selection of mobile phase and the cooperation of chromatographic column, the high performance liquid chromatography of different batches of shentong zhuyu decoction is compared, obtains the common peak containing loganic acid, amygdalin, hydroxyl safflower yellow pigment A, aniseed acid, qianghuo alcohol, isoeucommarin, ammonium glycyrrhizinate, kaempferol characteristic peak, and the fingerprint of shentong zhuyu decoction is constructed according to the common peak.The fingerprint constructed by the application can better detect 12 medicinal materials, shorten the detection time of single sample to 125 min or less, improve efficiency, and improve the quality control level of product.

The invention relates to the technical field of quality analysis of Chinese patent medicines, in particular to an establishment method and content determination method of HPLC characteristic chromatograms of related components of bone-strengthening musk analgesic ointment, and the establishment method comprises the following steps: step 1) preparation of a test solution; taking a bone-strengthening musk analgesic paste sample, pre-treating to remove a volatile matrix, adding a solvent, carrying out ultrasonic extraction, fixing the volume, filtering, and taking a subsequent filtrate to obtain a sample solution; 2) preparing a mixed reference substance solution; taking reference substances of aconitine, 4-methoxysalicylaldehyde, cinnamaldehyde, white angelica brain, gingerol, imperatorin and 6-shogaol, and adding methanol to a constant volume to obtain a reference substance stock solution; weighing the reference substance stock solution, and adding methanol to dilute the reference substance stock solution into a mixed reference substance solution; and (3) respectively measuring the sample solution in the step (1) and the mixed reference substance solution in the step (2) by adopting a high performance liquid chromatography, determining a common peak according to the relative retention time of a reference substance, determining a characteristic peak by utilizing a standard substance, and establishing the HPLC characteristic chromatogram of the bone-strengthening musk analgesic paste. The method has the advantages that the precision, the stability and the repeatability are good, the sample adding recovery rate meets the requirement, and the method can be used for actual quality control of the product.

The invention discloses a method for extracting high-purity imperatorin from angelica dahurica, which comprises the following steps: compounding angelica dahurica medicinal materials, performing hot reflux circulating extraction, reducing the degree, performing graded elution, concentrating, scheduling, freezing to remove impurities, reducing the degree, performing secondary graded elution, crystallizing, recrystallizing and performing vacuum drying to obtain an imperatorin pure product with the purity higher than 99%. The used raw materials are wide in source and easy to obtain, the used extraction equipment is common, the cost of the extraction solvent is low, the purification process is convenient and fast, an organic solvent with strong volatility and irritation is not used, and a novel green and environment-friendly industrial production method is provided for preparing the high-purity imperatorin.

This application discloses an epoxide hydrolase gene cloned from Angelica dahurica. AdEH1 and AdEH2 and its encoded proteins and applications, among which, AdEH 1 and AdEH2 The nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively, and the amino acid sequences encoding the proteins are shown in SEQ ID NO: 3 and SEQ ID NO: 4, respectively. AdEH1 and AdEH2 The encoded protein possesses epoxide hydrolase catalytic activity, capable of catalyzing the oxidation of imperatorin and angelica root to produce hydrated oxidized imperatorin and angelica root. This application identifies the gene. AdEH1 and AdEH2 Its key role in the biosynthesis of furanocoumarins provides core gene resources for the regulation and green large-scale synthesis of furanocoumarin components in Angelica dahurica, and also provides technical support for the accurate detection of related compounds.