12 results about "Imperatorin" patented technology

This invention belongs to the field of traditional Chinese medicine treatment, specifically relating to an anti-cold and detoxifying capsule pharmaceutical composition, its preparation method, and its application. The composition comprises the following components in parts by weight: 25-35 parts Angelica dahurica, 40-50 parts Scutellaria baicalensis, 2-5 parts flavonoids, 0.5-2 parts Imperatorin, 1-5 parts Artemisia capillaris, 1-5 parts phloroglucinol compounds, and 0.1-0.5 parts organic acids. The component-based composition provided by this invention has a good effect in preventing and treating wind-heat colds, and the components exhibit good synergistic effects.

This invention discloses a topical traditional Chinese medicine composition for relieving pain and its preparation method, belonging to the field of traditional Chinese medicine preparation technology. The topical traditional Chinese medicine composition for relieving pain is made from the following raw materials in parts by weight: Angelica sinensis 5-25 parts, Ligusticum striatum 5-20 parts, Lycopodium clavatum 10-35 parts, Artemisia argyi 5-25 parts, artificial Phellodendron chinense 3-18 parts, Clematis chinensis 10-40 parts, and Angelica dahurica 5-20 parts. This invention uses steam distillation to extract volatile oils and a dual-solvent gradient extraction method with the residue, which not only fully extracts the volatile fat-soluble components from Angelica sinensis, Ligusticum striatum, and Angelica dahurica, but also efficiently extracts the water-soluble components from artificial Phellodendron chinense, Lycopodium clavatum, and other medicinal materials. Experimental results show that the extraction rates of ferulic acid, ligustilide, imperatorin, and berberine hydrochloride in Example 1 of this invention reached 89.23%, 92.56%, 90.12%, and 93.45%, respectively, which are 110.7%, 223.1%, 75.8%, and 23.6% higher than those of conventional water decoction process, thus improving the utilization rate of raw materials.

The invention discloses a tissue regeneration promoting jade red gel and a quality detection method thereof.The tissue regeneration promoting jade red gel is characterized in that on the basis of a single-factor test, the dosage of carbomer 940, medicine and triethanolamine is used as influence factors, and the optimal proportion of all components of the gel is determined through an orthogonal test method: the dosage of carbomer 940 is 1%, the medicine loading capacity is 11%, and the dosage of triethanolamine is 1%; the results of accelerated stability and long-term stability tests show that the preparation has good stability in the investigation period. According to the method, the optimal thin-layer chromatography development condition is screened out, the radix angelicae sinensis, the radix arnebiae seu lithospermi and the sangusis draconis in the tissue regeneration promoting jade red gel are subjected to qualitative identification research, and chromatographic conditions such as the optimal flowability composition and a gradient elution mode are screened out through a large number of experiments; according to the present invention, with the high performance liquid chromatography method, the contents of the effective components such as ferulic acid, liquiritin, imperatorin, ligustilide, isoimperatorin and the like can be simultaneously determined, and the methodological detection results show that the high performance liquid chromatography method has characteristics of high precision, high accuracy, good repeatability and good stability.

The application discloses a method for constructing a characteristic map of a traditional Chinese medicine and the characteristic map, wherein the method adopts an HPLC method to determine the content of an effective component, imperatorin, in Zhikang capsules, and constructs a high performance liquid chromatography characteristic map of the Zhikang capsules; 10 characteristic peaks are determined as the characteristic map, which are respectively from dragon's blood, rhubarb and Bai Zhi; the components from the dragon's blood are dracorhodin B (peak 2) and pterostilbene (peak 4); the components from the rhubarb are peak 1 (aloe-emodin), peak 3, peak 6 (emodin), peak 9 and peak 10; and the components from the Bai Zhi are imperatorin (peak 5), peak 7 and peak 8; the method covers polyphenols, anthraquinones and furanocoumarins and other main components, and constructs a quality evaluation system of the Zhikang capsules; the method has good specificity, precision, repeatability and solution stability, and can be used for standardizing the production of the Zhikang capsules and guaranteeing the uniformity and stability of the internal quality of products to provide comprehensive quality control.

The application discloses a kind of shentong zhuyu decoction fingerprint construction method and application, belong to the technical field of drug detection.The application is detected by high performance liquid chromatography to several batches of shentong zhuyu decoction by the selection of mobile phase and the cooperation of chromatographic column, the high performance liquid chromatography of different batches of shentong zhuyu decoction is compared, obtains the common peak containing loganic acid, amygdalin, hydroxyl safflower yellow pigment A, aniseed acid, qianghuo alcohol, isoeucommarin, ammonium glycyrrhizinate, kaempferol characteristic peak, and the fingerprint of shentong zhuyu decoction is constructed according to the common peak.The fingerprint constructed by the application can better detect 12 medicinal materials, shorten the detection time of single sample to 125 min or less, improve efficiency, and improve the quality control level of product.

The invention relates to the technical field of quality analysis of Chinese patent medicines, in particular to an establishment method and content determination method of HPLC characteristic chromatograms of related components of bone-strengthening musk analgesic ointment, and the establishment method comprises the following steps: step 1) preparation of a test solution; taking a bone-strengthening musk analgesic paste sample, pre-treating to remove a volatile matrix, adding a solvent, carrying out ultrasonic extraction, fixing the volume, filtering, and taking a subsequent filtrate to obtain a sample solution; 2) preparing a mixed reference substance solution; taking reference substances of aconitine, 4-methoxysalicylaldehyde, cinnamaldehyde, white angelica brain, gingerol, imperatorin and 6-shogaol, and adding methanol to a constant volume to obtain a reference substance stock solution; weighing the reference substance stock solution, and adding methanol to dilute the reference substance stock solution into a mixed reference substance solution; and (3) respectively measuring the sample solution in the step (1) and the mixed reference substance solution in the step (2) by adopting a high performance liquid chromatography, determining a common peak according to the relative retention time of a reference substance, determining a characteristic peak by utilizing a standard substance, and establishing the HPLC characteristic chromatogram of the bone-strengthening musk analgesic paste. The method has the advantages that the precision, the stability and the repeatability are good, the sample adding recovery rate meets the requirement, and the method can be used for actual quality control of the product.

The invention relates to the field of angelica dahurica quality identification, and particularly discloses a method of an angelica dahurica quality evaluation model based on an artificial intelligence technology, which is characterized by comprising the following steps: S1, constructing fingerprints of different grades of angelica dahurica by utilizing imperatorin and isoimperatorin contents of the angelica dahurica; s2, collecting spectral images of the samples of the different grades of radix angelicae, constructing a shape feature database of the different grades of radix angelicae after background elimination and region-of-interest segmentation are completed, and processing data of the database to obtain optimized radix angelicae spectral data; and S3, training an artificial intelligence model by using the optimized angelica dahurica spectral data to obtain the angelica dahurica quality evaluation model. According to the method, a new technical means is provided for rapid, lossless and accurate quality identification and grade classification of the radix angelicae, and standardization and intellectualization of quality evaluation of the radix angelicae are realized.

The invention provides a method for detecting 14 components in fructus psoraleae, and belongs to the technical field of analysis and detection. The method comprises the following steps: performing alcohol extraction on fructus psoraleae to obtain a to-be-detected solution; performing high performance liquid chromatography detection on the liquid to be detected to obtain a chromatogram; and comparing the chromatogram with a standard substance spectrogram to obtain 14 components in the fructus psoraleae, the fructus psoraleae is prepared from the following 14 components: psoralen glycoside, isopsoralen glycoside, psoralen, isopsoralen, neopsoralen isoflavone, psoralen A, imperatorin, psoralen, psoralen chalcone, psoralen, psoralen B, psoralen flavanone methyl ether, psoralen isoflavone A and bakuchiol. The 14 components in the fructus psoraleae can be simultaneously distinguished and measured by controlling the composition and proportion of the mobile phase, the reference wavelength and the type of the chromatographic column, the detection method is rapid and efficient, and theoretical support is provided for distinguishing the type of processed products according to the content of different components subsequently.

The invention discloses a method for extracting high-purity imperatorin from angelica dahurica, which comprises the following steps: compounding angelica dahurica medicinal materials, performing hot reflux circulating extraction, reducing the degree, performing graded elution, concentrating, scheduling, freezing to remove impurities, reducing the degree, performing secondary graded elution, crystallizing, recrystallizing and performing vacuum drying to obtain an imperatorin pure product with the purity higher than 99%. The used raw materials are wide in source and easy to obtain, the used extraction equipment is common, the cost of the extraction solvent is low, the purification process is convenient and fast, an organic solvent with strong volatility and irritation is not used, and a novel green and environment-friendly industrial production method is provided for preparing the high-purity imperatorin.

This application discloses an epoxide hydrolase gene cloned from Angelica dahurica. AdEH1 and AdEH2 and its encoded proteins and applications, among which, AdEH 1 and AdEH2 The nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively, and the amino acid sequences encoding the proteins are shown in SEQ ID NO: 3 and SEQ ID NO: 4, respectively. AdEH1 and AdEH2 The encoded protein possesses epoxide hydrolase catalytic activity, capable of catalyzing the oxidation of imperatorin and angelica root to produce hydrated oxidized imperatorin and angelica root. This application identifies the gene. AdEH1 and AdEH2 Its key role in the biosynthesis of furanocoumarins provides core gene resources for the regulation and green large-scale synthesis of furanocoumarin components in Angelica dahurica, and also provides technical support for the accurate detection of related compounds.

The invention provides a method for determining the content of active ingredients in trifoliate orange. The method adopts high performance liquid chromatography (HPLC) to simultaneously determine naringin, trifoliate orange glycoside and imperatorin in the trifoliate orange. The method comprises the following steps: 1) preparing a reference solution and a test solution; 2) HPLC detection: adopting a DimonsilC18 chromatographic column, taking methanol-1% glacial acetic acid as a mobile phase for gradient elution, and controlling the flow rate to be 1.0 mL / min, the column temperature to be 30 DEG C and the detection wavelength to be 283 nm. The method has the beneficial effects that the three active ingredients of naringin, trifoliate glycoside and imperatorin in the trifoliate orange are simultaneously determined, and compared with single-ingredient determination, the quality of the trifoliate orange can be more comprehensively reflected; by optimizing chromatographic conditions and an extraction method, good separation and accurate determination of the three components are ensured, and the method has good specificity and linear relation, high accuracy and good reproducibility, and can provide reliable technical support for quality control, medicinal material screening and resource development of trifoliate orange.

The invention belongs to the technical field of detection of traditional Chinese medicine preparations, and particularly relates to a detection method for identifying adulteration of Angelica Koreana in a Qingconsciousness-relieving tablet. According to the detection method, decursin is taken as a detection index, HPLC is adopted for detection, and analysis and judgment are carried out according to a chromatographic peak area value of decursin. The method for detecting adulteration of the angelica koreana in the Qingconsciousness-relieving tablets is established by taking the decursin as a detection index and taking the content of the decursin contained in 10% of the angelica koreana adulteration in a Qingconsciousness-relieving tablet sample as a reference index, and a basis can be provided for quality monitoring of the Qingconsciousness-relieving tablets.