23 results about "Z-ligustilide" patented technology

The invention belongs to the technical field of traditional Chinese medicine preparation, and particularly relates to a peach kernel and safflower four-ingredient decoction compound preparation and a preparation method and a quality control method thereof. The invention provides a preparation method of a peach kernel and safflower four-component decoction compound preparation by screening and optimizing an enrichment method of volatile oil in extraction, concentration, inclusion and drying processes on the basis of an ancient water decoction process. According to the peach kernel and safflower four-drug decoction compound preparation prepared by the invention, the quality consistency with a reference sample is realized, including the consistency of each content index in the compound preparation with the reference sample and the consistency of a fingerprint spectrum with the reference sample; especially, characteristic peaks of volatile components-ligustilide, senkyunolide I and senkyunolide A are presented in the fingerprint spectrum, and the consistency of the content of the volatile oil is determined. Moreover, the quality control method provided by the invention is expected to be used for quality control of industrial large-scale production of a classic famous prescription, namely the peach kernel and safflower four-ingredient decoction compound preparation, and has a good application prospect.

The invention discloses a traditional Chinese medicine combination effective part capable of improving diabetic peripheral neuropathy. The traditional Chinese medicine combination effective part is prepared from a volatile oil effective part, a water-soluble effective part and an alcohol-soluble effective part, the effective part of the volatile oil comprises alpha-ocimene, alpha-santalene, bisabolol, beta-bisabolol and nerolidol; the water-soluble effective part comprises salvianolic acid A, salvianolic acid B and tanshinol; the alcohol-soluble effective part is prepared from Z-ligustilide, alpha-masticinic acid, beta-masticinic acid, tanshinone IIA, and 2-methoxy-5-acetyl-furangermacane-1 (10)-ene-6-ketone, and the alcohol-soluble effective part is prepared from the following raw materials in parts by weight: 2-methoxy-5-acetyl-furangermacane-1 (10)-ene-6-ketone. The traditional Chinese medicine composition effective part provided by the invention can regulate blood sugar, improve the conduction speed of sciatic nerves, repair damaged tissues, inhibit oxidative stress reaction, resist inflammation and relieve pain, so that the diabetic peripheral neuropathy is improved, the curative effect is reliable, the safety is good, the adverse reaction is low, and the traditional Chinese medicine composition effective part can be used for preparing a medicinal preparation for improving the diabetic peripheral neuropathy.

The application discloses a method for establishing a gynecological menstruation-regulating tablet fingerprint spectrum by using a high-performance liquid chromatography technology and application, by using the method, a good fingerprint spectrum is obtained, and the chromatographic peak is comprehensive, 12 fingerprint peaks are confirmed, wherein, corresponding attribution peaks of angelica sinensis, fried atractylodes macrocephala koidz, vinegar corydalis and chuanxiong are all in the gynecological menstruation-regulating tablet, and the angelica sinensis, fried atractylodes macrocephala koidz and vinegar corydalis all have exclusive peaks. By comparing with the reference substance, and combining with ultraviolet absorption spectrum characteristics, characteristic peaks of five chemical components, i.e., chlorogenic acid, ferulic acid, chuanxiongolide I, ligusticum lactone and butenyl phthalide are identified. The application further discloses a reference fingerprint spectrum of the gynecological menstruation-regulating tablet obtained by using the method, the gynecological menstruation-regulating tablet fingerprint spectrum detection method and the obtained reference fingerprint spectrum can objectively evaluate the overall quality of the gynecological menstruation-regulating tablet, and provide a research basis for quality control of the gynecological menstruation-regulating tablet.

The application discloses a composition for treating lumbar intervertebral disc herniation and repairing annulus fibrosus, relates to the technical field of traditional Chinese medicine compositions, and comprises the following raw materials: radix rehmanniae preparata, plastrum testudinis, fructus psoraleae, eucommia ulmoides oliver, walnut kernel, bee pollen, ligustilide, saikosaponin A, salvianolic acid B, ferulic acid, calycosin, stibene glycoside, prunol, loganin, hydroxysafflor yellow A and ligustilide. Experiments show that the composition can strengthen the repair of annulus fibrosus, has anti-inflammatory and analgesic effects, protects collagen, inhibits degeneration, can effectively treat lumbar intervertebral disc herniation and realize the structural repair of damaged annulus fibrosus, and provides a new technical scheme for the clinical treatment of lumbar intervertebral disc herniation.

The invention provides a method for extracting phthalide chemical components in angelica sinensis, which comprises the following steps of: firstly, shearing and emulsifying the angelica sinensis at a high speed, carrying out high-speed shearing and extraction by using petroleum ether and ethyl acetate as extraction solvents, and recovering the solvents from the extract under reduced pressure through a rotary evaporator to obtain angelica sinensis essential oil; wherein the extracted essential oil contains senkyunolide A, ligustilide and butylphthalide. Aiming at the characteristics that the phthalide component in the angelica volatile oil is thermally unstable and is easy to oxidize, the phthalide component in the angelica volatile oil can be rapidly extracted under a low-temperature condition by combining a petroleum ether-ethyl acetate solution system with a high-speed shearing, dispersing and emulsifying technology, and the activity of the angelica volatile oil can be better maintained. The method has the characteristics of low temperature, simplicity in operation, short time and low cost.

This invention relates to the field of pharmaceutical technology, specifically proposing the application of ligustilide in the preparation of drugs for inhibiting and / or preventing and / or alleviating and / or treating lung tumors to address the current lack of natural drugs for lung cancer prevention. Lung tumors are nitrosamine-induced carcinogenesis of lung tissue. The invention relates to a pharmaceutical preparation for inhibiting and / or preventing and / or alleviating and / or treating lung tumors, comprising an active ingredient and pharmaceutically acceptable excipients; wherein the active ingredient is ligustilide. Ligustilide directly targets the STING protein, activates the cGAS-STING pathway, promotes STING phosphorylation and cell-mediated immune surveillance, while simultaneously regulating ROS homeostasis and the inflammatory microenvironment, reducing nitrosamine damage to lung epithelial cells, and preventing nitrosamine-induced lung carcinogenesis.

The invention belongs to the technical field of biopesticides, and particularly relates to a biopesticide for preventing and treating citrus thrips. The invention relates to a biopesticide. Active ingredients of the biopesticide are formed by compounding ligustilide and rotenone, or the active ingredients of the composition are formed by compounding ligustilide and sophocarpidine. The ligustilide is compounded with the rotenone and the sophocarpidine respectively, the combined action effect of the compounded combination on the citrus thrips is measured through a leaf tube medicine membrane method, and the result shows that when the ligustilide and the rotenone are compounded according to the mass ratio of (1-15): (15-1), the synergistic interaction effect on the citrus thrips is achieved; when ligustilide and sophocarpidine are compounded according to the mass ratio of (1-9): 1, the ligustilide and sophocarpidine have a synergistic effect on the citrus thrips. According to the present invention, the ligustilide and the rotenone or the sophocarpidine are compounded so as to improve the prevention and control effect on the citrus thrips and delay the development of the drug resistance of the pests, such that the support can be provided for the research and development of the biopesticide for preventing and controlling the citrus thrips.

This invention discloses a method for determining the content of a traditional Chinese medicine composition containing Angelica sinensis and cinnamon. The method includes the following steps: taking a test solution and a reference solution of the traditional Chinese medicine composition for detection; the chromatographic conditions for detection are: using a chromatographic column packed with octadecyl bonded silica gel; mobile phase A selected from one or more of acetonitrile, methanol, and tetrahydrofuran; and mobile phase B being an acidic aqueous solution, an alkaline aqueous solution, and / or a buffer salt aqueous solution; obtaining the content information of the reference standard of the traditional Chinese medicine composition based on the detection results; wherein the traditional Chinese medicine composition includes Angelica sinensis, cinnamon, white peony root, ginger, jujube, and licorice; and the reference standard is ferulic acid, cinnamaldehyde, 6-gingerol, and ligustilide. The detection method of this invention is simple, accurate, efficient, stable, precise, reproducible, convenient, and easy to master. It can effectively save energy and reduce analytical costs, while the prior art does not disclose this technical content.

The invention discloses a fingerprint spectrum detection method of a consistent decoction reference sample and application, and relates to the technical field of traditional Chinese medicine detection. According to the fingerprint detection method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 28-32 DEG C; the detection wavelength is 0 to 25 minutes, and the wavelength is 278 to 282nm; in 25-66 minutes, the wavelength is 318-322 nm; the time is 66 to 80 minutes, and the wavelength is 278 to 282nm; the time is 80 to 90 minutes, and the wavelength is 318 to 322 nm; the mobile phase A is acetonitrile, and the mobile phase B is a 0.09-0.11 wt% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the method, digitonin C, 4-cumaric acid, ferulic acid, rutin, verbascoside, senkyunolide I and ligustilide in the consistent decoction can be detected at a time, and the method is high in accuracy, good in repeatability, high in durability and good in specificity.

The invention discloses a GC-MS (Gas Chromatography-Mass Spectrometer) method for determining effective components in an aromatic Wentong formula. A chromatographic column is a capillary column; a temperature programming method is adopted; the ionization mode in the mass spectrum condition is electron impact ionization, and the acquisition mode is scanning. According to the method disclosed by the invention, the active ingredients of the aromatic Wentong prescription can be effectively separated in a gas chromatograph through a specific heating program in combination with specific sample inlet temperature, chromatographic column temperature and split ratio and can be better focused and enter a mass spectrometer at the same time, and under the participation of specific mass spectrometry conditions, the effective separation of the active ingredients of the aromatic Wentong prescription can be realized. According to the present invention, with the method, the interference of the cinnamyl aldehyde, the alpha-caryophyllene, the methyleugenol and the ligustilide in the chromatogram can be eliminated, the signal strength and the sensitivity are high, the accurate quantification of the four components such as the cinnamyl aldehyde, the alpha-caryophyllene, the methyleugenol and the ligustilide is finally achieved, the reference basis is provided for the quality control of the aromatic warming and dredging formula, and the practical application value is provided.

The invention relates to the technical field of anti-alopecia products, and particularly provides an angelica sinensis-cacumen biotae compound nanoemulsion and a preparation method and application thereof.Angelica sinensis-cacumen biotae mixed volatile oil serves as an oil phase, an angelica sinensis polysaccharide aqueous solution serves as a water phase, in order to reduce the dosage of an additive, OP-10 is screened out to serve as a surface active agent, PEG-400 serves as a cosurfactant, and the angelica sinensis-cacumen biotae compound nanoemulsion is prepared. The stable and uniform nano-emulsion system is prepared by specifically adjusting the adding proportion of all the raw materials, the nano-emulsion is clear and transparent light yellow liquid oil-in-water type emulsion, the particle size of the emulsion is smaller than 100 nm, the pH value of the emulsion is close to the normal pH value of human scalp, and the nano-emulsion is moderate in viscosity and easy to smear and meets the requirements of transdermal drug delivery preparations. Meanwhile, the research finds that the nanoemulsion has high retention characteristic, can continuously release Z-ligustilide at the administration site, has higher local treatment effect, and has good skin safety. The nanoemulsion not only can promote hair growth, but also can improve metabolism by regulating blood fat.

The invention discloses a pharmaceutical composition for treating acute myocardial infarction, a preparation and application. The medicine composition is prepared from raw material medicines including Z-ligustilide and chlorogenic acid, and the molar ratio of the Z-ligustilide to the chlorogenic acid is 5: (1-20). The pharmaceutical composition disclosed by the invention can be used for treating acute myocardial infarction in a synergistic manner.

The invention provides a temperature-sensitive hydrogel preparation for treating brain hypoxia related diseases, the active ingredient of the temperature-sensitive hydrogel preparation is a ligustilide cyclodextrin inclusion compound, and the temperature-sensitive hydrogel preparation is prepared from ligustilide and hydroxypropyl-beta-cyclodextrin through an inclusion effect. Compared with the prior art, the invention has the following advantages: the cyclodextrin inclusion technology can effectively improve the problems of low stability, poor solubility and the like of ligustilide, and the poloxamer temperature-sensitive hydrogel has good stability, biocompatibility and slow release performance. The cyclodextrin inclusion compound and the hydrogel are combined to be applied to the nasal preparation, after the temperature-sensitive hydrogel enters the nasal cavity, the gel form at the physiological temperature is converted into a semi-solid and is adsorbed on the nasal mucosa, the residence time of the medicine at the administration position is prolonged, the slow release effect is achieved, meanwhile, the administration dosage can be reduced, the side effect of the medicine is relieved, and the bioavailability of the medicine is improved. The compound can be used for various brain diseases, is expected to overcome the defects of the traditional nasal preparation, and improves the curative effect of the medicine and the acceptability of a patient.

The application discloses a composition for treating depression and application thereof, and the composition for treating depression comprises the following components in parts by weight: ligustilide 35-40 parts, arturn 0.5-1.5 parts and alpha-cyperone 0.1-1 part; the application firstly compounding active ingredients in Yujia pills which target 5-HT1AR and 5-HTT, and preparing a new traditional Chinese medicine efficacy component composition; in addition, the anti-depression efficacy of the active ingredients is evaluated by establishing a reserpine mouse depression model, and it is shown that the active ingredient composition screened from the 5-HT1AR and 5-HTT targets can regulate the content of monamine neurotransmitters in brain tissues to exert the anti-depression efficacy, and the in-vivo pharmacological experiment fully proves the anti-depression efficacy of the composition.

The present invention relates to a method for constructing a characteristic spectrum of volatile oil in Qingshang Juantong Decoction and a method for detecting Qingshang Juantong Decoction samples. By using an ultra-high performance liquid chromatography (ULPC) detection method, the chromatographic conditions are rationally controlled to confirm the common peaks of the Qingshang Juantong Decoction standard decoction, and to construct a characteristic spectrum of volatile oil in Qingshang Juantong Decoction. The characteristic spectrum of the present invention comprises 12 characteristic peaks, wherein peak 8, peak 9, and peak 11 are reference substances methyl eugenol, ligustilide A, and ligustilide, respectively. The method is widely applicable to the quality inspection of Qingshang Juantong Decoction benchmark samples, preparation intermediates, and / or finished preparations, can more comprehensively reflect product quality, has high analysis efficiency, is convenient to operate, and is suitable for quality monitoring of continuous production.

The invention provides functional aromatherapy essential oil and a preparation device thereof, the functional aromatherapy essential oil comprises a neurotransmitter regulation and control group, a meridian stimulation group and a biological rhythm regulation group, the neurotransmitter regulation and control group comprises 5-15 parts of lavender essential oil and 3-10 parts of Roman chamomile essential oil; according to the invention, a composite system of a neurotransmitter regulation group, a meridian stimulation group and a biological rhythm regulation group is adopted, and all the components form a multi-target synergistic effect through a specific ratio; according to the supercritical CO2 extraction technology of ligusticum wallichii, it is ensured that the ligustilide content is larger than or equal to 8%; the evening primrose seed oil is encapsulated by a beta-cyclodextrin embedding technology, so that gamma-linolenic acid is effectively prevented from being oxidized, and the shelf life of the product is prolonged; according to the formula, transmission of neurotransmitters is regulated and controlled through linalyl acetate and alpha-bisabolol, meridian circulation is stimulated by means of d-limonene, the secretion period of human melatonin is synchronized through benzyl benzoate, three-dimensional adjustment of nerves, meridians and rhythms is achieved, and the limitation of traditional single-target action is broken through.

The present application belongs to the field of medicine and health care products, and particularly relates to a pharmaceutical composition for treating atherosclerosis and application thereof. The pharmaceutical composition is mainly composed of tectoridin and Z-ligustilide, and the molar ratio of the two is (1-5):(1-5). In vivo experiments are carried out on ApoE ‑ / ‑ mouse models, and the results show that after the composition is administered by gavage, it can significantly inhibit the formation of aortic plaques, reduce the necrotic core area in the plaques, and reduce the recruitment of macrophages under the vascular endothelium. The pharmaceutical composition has a clear anti-atherosclerotic effect, can effectively improve the pathological process, and is expected to be developed into a new drug for clinically treating atherosclerosis.

The invention discloses a preparation method and application of a natural Chinese herbal medicine compound feed for improving disease resistance of Tan sheep. The core is a five-in-one system of angelica sinensis, Chinese wolfberry fruits, probiotics, a slow-release carrier and a stress protective agent. The Chinese angelica is used as a core to directionally enrich ferulic acid and ligustilide, Chinese wolfberry, betaine, alpha-ketoglutaric acid and Ningxia salt-tolerant bacillus subtilis are combined, and a modified alfalfa fiber slow-release carrier containing a yeast culture and magnesium oxide is embedded. The additive amount is adjusted through grading stress states of respiratory rate and excrement form, so that the targeted release of active ingredients is realized, the anti-stress capability and intestinal health of the Tan sheep are synergistically enhanced, the disease resistance in the stress period is remarkably improved, the disease incidence rate is reduced, no drug residue exists, the preparation process is mature, and the feed is suitable for green large-scale breeding and has a wide application prospect.

The application belongs to the technical field of medicine, and particularly relates to application of pioglitazone combined with ligustilide in prevention and treatment of metabolic related fatty liver fibrosis. Specifically, the application first evaluates the protective effect of ligustilide alone in the liver fibrosis caused by high-fat and high-sugar stimulation in vivo and in vitro. Meanwhile, in the evaluation process of improving related liver fibrosis damage of the pioglitazone and ligustilide drug combination, the high-fat and high-sugar model closely related to glucose and lipid metabolism disorder is innovatively constructed and selected. It is found that the curative effect of the combination of pioglitazone and ligustilide is significantly better than that of any one of the drugs used alone, and the combination exhibits a strong synergistic effect in inhibiting lipid synthesis, promoting lipid metabolism, regulating glucose metabolism and the like, so that a more effective and innovative prevention and treatment method is provided for clinical treatment of metabolic related fatty liver fibrosis, and therefore has important clinical significance and social benefits.

The invention relates to a strontium-containing growth regulator and application thereof in improving stress resistance of plants, and belongs to the technical field of plant growth regulators. The growth regulator is composed of a component A and a component B. The component A contains 0.3-0.5 part by weight of jasmonic acid, 0.2-0.25 part by weight of ligustilide, 1-1.5 parts by weight of a bunge pricklyash leaf extract, 1-1.5 parts by weight of a Chinese prickly ash leaf extract, and the balance of water. The component B is prepared from 10 to 12 parts by weight of microbial extract, 0.1 to 0.3 part by weight of choline chloride, 15 to 20 parts by weight of potassium fulvate, 1 to 1.5 parts by weight of betaine, 5 to 8 parts by weight of Irpex lacteus polysaccharide, 100 parts by weight of water and 10 to 15 parts by weight of surfactant. When the growth regulator is used for soaking plant seeds or treating plant seedlings, the germination rate of the seeds in a low-temperature environment can be obviously increased, and the growth performance of the seedlings in the low-temperature environment is improved.

The invention belongs to the technical field of cardiovascular disease treatment medicines, and particularly relates to application of ligustilide in preparation of a medicine for relieving inflammatory response after myocardial ischemia. According to the application disclosed by the invention, research finds that ligustilide can be used for remarkably inhibiting the level of S100A9 in serum, remarkably inhibiting expression of S100A9 mRNA and protein in a cardiac infarction region and expression of S100A9 in neutrophil for the first time, so that release of inflammatory factors and chemotactic factors can be effectively reduced, and occurrence of myocardial tissue inflammatory response is prevented or inflammatory response caused by myocardial ischemia is relieved; the compound can inhibit MI mouse infarction region neutrophil and mononuclear cell infiltration, and inhibit formation of NETs after MI and expression and activation of NLRP3 inflammasome, so that inflammatory response after MI can be relieved, and myocardial tissue can be protected. The application range of ligustilide in cardiovascular diseases is expanded, and a new drug choice is provided for prevention and treatment of inflammatory response after myocardial ischemia.

The invention belongs to the field of medicines, and particularly relates to application of Z-ligustilide in a medicine for treating migraine. Z-ligustilide is combined with fecal fungus transplantation (FMT), by adjusting host intestinal flora and metabolites, the community structure and metabolic function of the intestinal flora can be recovered, the level of neurotransmitters such as serotonin can be adjusted, the migraine symptom can be relieved through the multi-target effect, the clinical symptom of migraine can be improved through multiple ways through the Z-ligustilide combined fecal fungus transplantation technology (FMT), and the migraine treatment effect is improved. The treatment effectiveness is improved.

The invention provides supramolecular repair essence and a preparation method thereof, and belongs to the technical field of cosmetics. The supramolecular repairing essence is prepared from the following components in parts by weight: 0.2 to 0.5 part of a supramolecular composition, 5 to 10 parts of a plant extract, 10 to 20 parts of a humectant and 40 to 80 parts of water, the preparation method of the supramolecular composition comprises the following steps: (1) dissolving hydroxypropyl alpha-cyclodextrin in water, then adding calendaring element and ligustilide, and stirring at 60 DEG C for 3 hours to obtain a mixed solution; and (2) carrying out spray drying on the mixed solution to obtain the supramolecular composition, according to the invention, the supramolecular composition and the plant extract are mixed, and the specific variety of the plant extract and the mass ratio of the components are controlled, so that the obtained essence not only has a better repairing effect, but also has obviously enhanced moisturizing and antibacterial effects.