Method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity, and use

A chuanxiong extract and a technology of chuanxiong, which are applied in the application field of preparing non-alcoholic fatty liver treatment drugs, can solve problems such as unclearness, and achieve the effects of simple and clear chemical composition, easy quality control, and good pharmacological activity.

Inactive Publication Date: 2012-12-19
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current researches are focused on ligustrazine and ferulic acid, and it is not clear whether liguslides have the activity of inhibiting lipid accumulation in liver cells.

Method used

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  • Method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity, and use
  • Method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity, and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one The preparation of effective component of Rhizoma Chuanxiong

[0031] After crushing 1000g of Rhizoma Chuanxiong medicinal material, add 4000ml of 95% ethanol to soak overnight, reflux extraction for 2 hours, then add 4000ml of 95% ethanol to heat and reflux for extraction three times, each time for 2h, combine the extracts, and concentrate to a density of 1.08. Add the concentrated solution to 500ml of water to disperse into a suspension, add 500ml of petroleum ether to extract three times, combine the extracts, and recover the solvent to dryness. Take part of the extract extracted with petroleum ether, add normal phase silica gel to mix the sample, pass it through a normal phase silica gel column, and use petroleum ether (60-90) and ethyl acetate solution with a volume ratio of 50:1 as the eluent to elute, collect The eluate was concentrated to obtain 11.3 g of a light yellow liquid, which is the Rhizoma Chuanxiong extract of the present invention, whic...

Embodiment 2

[0032] Embodiment two The analysis of the effective components of Rhizoma Chuanxiong

[0033] Weigh and dissolve the Rhizoma Chuanxiong extract as shown in Example 1, and transfer an appropriate amount to a brown liquid phase sampling bottle for liquid chromatography analysis.

[0034]The analysis conditions are as follows: Agilent 1100 liquid chromatograph, injection volume 10 μl; flow rate 1ml / min, column temperature 30°C. The chromatographic mobile phase gradient is: 0~40min: water (0.1% formic acid): 70%~20%, acetonitrile: 30%~80%; 40-50min: water (0.1% formic acid): 20%~5%, acetonitrile: 80%~95%; 50~55min: water (0.1% formic acid): 5%, acetonitrile: 95%; detection wavelength 271nm. The analytical spectrum is attached figure 1 shown. Liquid chromatographic analysis showed that the extract of Chuanxiong Rhizoma had an obvious chromatographic peak at 26.5min, and the compound represented by this peak was identified as ligustilide after comparison with the standard. The ...

Embodiment 3

[0035] Embodiment three Rhizoma Chuanxiong effective component preparation

[0036] Take 0.5 g of the effective components of Ligusticum chuanxiong obtained in Example 1 and mix evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and after the chemical material is moved to drip irrigation, the drug solution is dropped into 6 ~ 8 ℃ of liquid paraffin, and the Oil, made 400 drop pills.

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PUM

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Abstract

The invention relates to a method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity and a use, especially to a Ligusticum wallichii extract containing 30 to 90 % of ligustilide. The extract is prepared by the following method comprising smashing the Ligusticum wallichii medicinal materials, immersing in 70 to 99 % ethanol, e.g. 75 to 98 %, extracting by reflux for 1 to 4 times, combining extract, condensing, extracting by using petroleum ether, recovering the solvent of the combined extract till dryness to get a petroleum ether extract; extracting a part of the extract by using the petroleum ether, adding normal phase silica gel to stir, going through a normal phase silica gel column, eluting by using the petroleum ether and an ethyl acetate solution with a volume ratio of 50 : 0.5-5 as eluents, collecting the eluates, and condensing to get the Ligusticum wallichii lactone extract.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and in particular relates to an effective component extracted from Rhizoma Chuanxiong and its preparation method, as well as the application of the component in the preparation of non-alcoholic fatty liver medicine. Background technique [0002] Liver injury is a complex process caused by multiple etiologies and involving multiple factors. Liver disease caused by liver injury has become a common and frequently-occurring disease. What is more serious is the liver failure and hepatic encephalopathy caused by it. The morbidity and mortality are high, which greatly threatens human health. Many factors can cause liver damage. Nonalcoholic fatty liver disease (NAFLD), also known as nonalcoholic fatty liver (NAFL), is a disease characterized by fatty degeneration and fat accumulation in hepatic parenchymal cells without a history of excessive drinking clinicopathological syndrome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/236A61K31/365A61P1/16A61K125/00
Inventor 程翼宇张萌王怡王毅刘二伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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