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A method for extracting and separating astaxanthin from Haematococcus pluvialis by a two-phase coupled wall-breaking technology

A technology of Haematococcus pluvialis and Haematococcus pluvialis powder, applied in the direction of organic chemistry, etc., can solve the problems of cumbersome separation and purification process of macroporous resin, difficult recovery of astaxanthin, and many impurities in the extract, and achieve subsequent purification Ease of handling, reduced types and amounts, and high purity

Inactive Publication Date: 2017-01-25
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Considering the thermal sensitivity of astaxanthin, the high-pressure homogenization wall breaking method and ultrasonic wall breaking method can lead to the inactivation of astaxanthin due to temperature rise.
At present, the method of repeated freezing and thawing is commonly used to break the wall, but the particle size of the algae residue in the repeated freezing and thawing method is small, the slurry is difficult to separate by traditional filtration technology, and there are many impurities in the extract, and the subsequent separation and purification process of macroporous resin is tedious. , many reagents used, difficult recovery of astaxanthin and other problems, it is necessary to develop new extraction and separation methods

Method used

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  • A method for extracting and separating astaxanthin from Haematococcus pluvialis by a two-phase coupled wall-breaking technology
  • A method for extracting and separating astaxanthin from Haematococcus pluvialis by a two-phase coupled wall-breaking technology
  • A method for extracting and separating astaxanthin from Haematococcus pluvialis by a two-phase coupled wall-breaking technology

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Effect test

Embodiment 1

[0038] The freeze-thaw wall-breaking technology of the present invention-a two-phase system integration method for extracting and separating astaxanthin from Haematococcus pluvialis is as follows:

[0039] (1) Add ammonium sulfate solution to the Haematococcus pluvialis powder, the solid-to-liquid ratio of the two is 1:1g / ml, then soak for 0.5h, then place it in a quick-freezer to quickly cool to -15°C, and freeze for 15h;

[0040] (2) After freezing, take it out, thaw at room temperature, then add 3ml of propanol per gram of dry algae powder, place it on a shaker at 180r / min for 40min, and let it stand for 5min;

[0041] (3) After the phase separation is complete, remove the propanol upper phase, put the brine phase and algae powder back into the quick-freezer, repeat the freezing, propanol extraction, and phase separation three times according to steps (1) and (2), Collect all the propanol obtained and evaporate the propanol by vacuum distillation, add ethanol, and vacuum-dr...

Embodiment 2

[0045] The freeze-thaw wall-breaking technology of the present invention-a two-phase system integration method for extracting and separating astaxanthin from Haematococcus pluvialis is as follows:

[0046] (1) Add ammonium sulfate solution to the Haematococcus pluvialis powder, the solid-to-liquid ratio of the two is 1:5g / ml, then soak for 3 hours, then place it in a quick freezer to quickly cool to -25°C, and freeze for 10 hours;

[0047] (2) After freezing, take it out, thaw at room temperature, then add 9ml of propanol per gram of dry algae powder, shake on a shaker at 200r / min for 20min, and let stand for 15min;

[0048] (3) After the phase separation is complete, remove the propanol upper phase, put the brine phase and algae powder back into the quick-freezer, repeat the freezing, propanol extraction, and phase separation three times according to steps (1) and (2), Collect all the propanol obtained and evaporate the propanol by vacuum distillation, add ethanol, and vacuum...

Embodiment 3

[0052] The freeze-thaw wall-breaking technology of the present invention-a two-phase system integration method for extracting and separating astaxanthin from Haematococcus pluvialis is as follows:

[0053](1) Add ammonium sulfate solution to the Haematococcus pluvialis powder, the solid-to-liquid ratio of the two is 1:3g / ml, then soak for 1 hour, then place it in a quick freezer to quickly cool to -20°C, and freeze for 12 hours;

[0054] (2) After freezing, take it out, thaw at room temperature, then add 6ml of propanol per gram of dry algae powder, shake on a shaker at 190r / min for 30min, and let stand for 10min;

[0055] (3) After the phase separation is complete, remove the propanol upper phase, put the brine phase and algae powder back into the quick-freezer, repeat the freezing, propanol extraction, and phase separation three times according to steps (1) and (2), Collect all the propanol obtained and evaporate the propanol by vacuum distillation, add ethanol, and vacuum-d...

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Abstract

The invention discloses a method for extracting astaxanthin from haematococcus pluvialis by a double-aqueous-phase coupled wall breaking technology. A double-aqueous-phase separating system is coupled with a freeze-thawing wall breaking technology through experiments, and the processes of wall breaking, extracting and separating are coupled through the introduction of the double-aqueous-phase system. The astaxanthin can be effectively separated from algae residues and various impurity components by a simple liquid separating method during wall breaking and extracting, and the purity and the extracting rate of the astaxanthin are effectively improved. The extracting and separating processes are reduced, and meanwhile, a freeze-thawing agent and an extractant are consistent with the phase forming substances of the double-aqueous-phase system. The types and the dosage of reagents are reduced, and the used reagents are environment-friendly and easy to recycle.

Description

technical field [0001] The invention relates to the technical field of chemical extraction, in particular to a method for extracting and separating astaxanthin from Haematococcus pluvialis using a two-phase coupling wall breaking technology. Background technique [0002] Astaxanthin is the only carotenoid that can pass through the blood-brain barrier. Studies have shown that the antioxidant activity of astaxanthin is more than 10 times higher than that of β2 carotene, and it is also higher than vitamin E in inhibiting lipid peroxidation. More than 100 times, known as "super antioxidant", has been widely used in health food, medicine, cosmetics and aquaculture industry, with extremely high economic value and health care effect. Haematococcus pluvialis is recognized as the best biological source of natural astaxanthin in nature. The astaxanthin content of Haematococcus pluvialis can reach more than 3%, which is much higher than other raw materials. It is extracted from Haemato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 高云涛熊华斌刘晓海彭金辉李晓芬刘晓芳马肖艳杨洪巧张宏教
Owner YUNNAN MINZU UNIV
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