The invention discloses a red ginseng extract. The extract is characterized by containing ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, notoginsenoside R2 and ginsenoside Ro. A preparation method of the extract comprises the following steps: cutting red ginseng serving as a medicinal material into pieces, adding water in an amount which is 3-10 times that of the red ginseng, soaking for 0.5-2 hours, heating and performing reflux extraction for 1-2 hours, extracting for 1-3 times, combining extraction solutions, filtering and concentrating; separating and purifying a concentrated solution with a reverse phase silica gel column; eluting with 30-40 percent methanol-water by 4-10L, and discarding; and eluting with 50-70 percent methanol-water by 4-6L, collecting an eluent and concentrating to obtain active ingredients.