Effective component of Rabdosia amethystoides and preparation and use thereof
An effective component, a technology of fragrant tea, which is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects, involvement of normal cells, etc.
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Embodiment 1
[0128] The preparation of the effective component of embodiment 1 fragrant tea
[0129] Take 250 g of fragrant tea and vegetables, crush it, add ethyl acetate and ethanol (1:1), heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 25.5g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 6.6 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18 ; 21.2mm × 250mm), the mobile phase is water (A) and acetonitrile (B), and the gra...
Embodiment 2
[0132] The analysis of the effective component of embodiment 2 fragrant tea dish
[0133] The HPLC-ELSD joint analysis of the effective components of the fragrant tea dish of embodiment 1
[0134] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase...
Embodiment 3
[0137] Embodiment 3 Fragrant tea active ingredient preparation
[0138] Take the active ingredient 1 or 2 of the fragrant tea dish in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, move the material to the drip irrigation after the material is made, and drop the medicine liquid to 6-8°C In liquid paraffin, degreasing, made 400 dropping pills.
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