131results about How to "Ease of elucidation of mechanism of action" patented technology

The present invention relates to an effective component of rabdosia and the preparation method and usage. For the effective component of rabdosia of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract rabdosia; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 20.0 to 24.0min or 24.0 to 28.0min so as to obtain the effective component.

The present invention relates to an effective component of root of subprostrate sophora and the preparation method and usage. For the effective component of root of subprostrate sophora of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract the root of subprostrate sophora; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 36.0 to 40.0min so as to obtain the effective component.

The invention relates to an effective component of artemisia leaves as well as a preparation method and an application thereof. The preparation process of the effective component of the artemisia leaves comprises the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the artemisia leaves; (2) dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 28.0-32.0 minutes, 36.0-40.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 52.0-60.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

The invention discloses an effective component for nutgall, a preparation method of the same and application of the same in resisting tumor. The effective component is obtained through the following steps that: the extraction is performed on the nutgall through a mixture of ethyl acetate and ethanol which is taken as a solvent, and an extracting solution is obtained; the extracting solution is subjected to chromatography in a chromatographic column to obtain an eluent; and the eluent is subjected to gradient elution by preparing liquid-phase chromatography, a mobile phase is water and acetonitrile, and the eluent is collected for 48.0 to 52.0 minutes or 52.0 to 56.0 minutes.

The invention relates to effective components of Cacumen Platycladi as well as a preparation method and a purpose thereof. The preparation process comprises the following steps: 1. the mixer of ethyl acetate and ethanol is used as a solvent for extracting Cacumen Platycladi; 2. eluent is obtained from an extracting solution through chromatographic column chromatography; 3. with the moving phase ofwater and acetonitrile, the eluent obtained by the phase color chromatographic gradient elution of a preparation solution is collected for 36.0-40.0 minutes, and the effective components are obtained from the eluent after 44.0-48.0 minutes.

The invention relates to effective components of an airpotato yam rhizome, a preparation method and the application thereof. The process for preparing the effective components of an airpotato yam rhizome comprises the following steps: step 1, the airpotato yam rhizome is extracted by the mixture of ethyl acetate and ethanol, which is used as a solvent; step 2, medicine residues are extracted by ethanol to obtain extracting solution; step 3, extracting solution is treated with column chromatography to obtain elution solution; step 4, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 10.8-13.8 min is collected to obtain the effective components.

The present invention relates to an effective component of Rhizoma Atractylodis and the preparation method and usage. For the effective component of Rhizoma Atractylodis of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Rhizoma Atractylodis; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 28.1 to 33.9min so as to obtain the effective component.

The present invention relates to an effective component of leatherleaf millettia and the preparation method and usage. For the effective component of leatherleaf millettia of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract leatherleaf millettia; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 32.6 to 40.8min so as to obtain the effective component.

The present invention relates to an effective component of Common Andrographis Herb and the preparation method and usage. For the effective component of Common Andrographis Herb of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Common Andrographis Herb; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 25.5 to 33.8min so as to obtain the effective component.

The invention relates to an effective component of red peony root, a preparation method and application thereof; the preparation process of the effective component of red peony root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting red peony root; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 28.6-32.2 min is collected to obtain the effective component.

The present invention relates to an effective component of rabdosia rubescens and the preparation method and usage. For the effective component of rabdosia rubescens of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract rabdosia rubescens ; step 2: the extract goes column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 15.5 to 19.6min so as to obtain the effective component.

The invention relates to a reed rhizome effective component, a preparation method thereof and a use, and the preparation process comprises the following steps: step 1: mixture of ethyl acetate and ethanol is taken as a solvent to extract reed rhizome; step 2: eluent is obtained by leading extract liquid to pass through a chromatographic column for chromatography; and step 3: the eluent which is obtained by using the liquid-phase chromatographic gradient elution with the mobile phase of water and acetonitrile is used for collecting the eluent at 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 60.0-64.0 minutes for obtaining the effective component.

The invention relates to an effective component of cogongrass rhizome as well as a preparation method and an application thereof. The preparation process of the effective component of the cogongrass rhizome comprises the following steps: (1)a mixture of ethyl acetate and ethanol is used as a solvent to extract the cogongrass rhizome; (2) dregs of a decoction are extracted with the ethanol so as toobtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogra m gradient and is collected for 4.0-8.0 minutes, 8.0-12.0 minutes, 20.0-24.0 minutes, 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 36.0-40.0 minutes, 40.0-44.0 minutes, 44.0-48.0 minutes; 48.0-52.0 minutes, 52.0-56.0 minutes, 56.0-60.0 minutes and 60.0-64.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

The invention relates to an effective component of prunella vulgaris, a preparation method and application thereof; the preparation process of the effective component of prunella vulgaris in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting prunella vulgaris; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 24.0-28.0 min or 28.0-32.0 min is collected to obtain the effective component.