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131results about How to "Ease of elucidation of mechanism of action" patented technology
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Extract of an traditional Chinese medicine containing isopsoralen, preparation method and use thereof
InactiveCN101007050AGood inhibitory effectThe chemical composition is simple and clearAntineoplastic agentsHeterocyclic compound active ingredientsAngelicinDrug
The invention has provided Chinese traditional medicine extract which contains 10%-24% of angelicin, 8-19% part of psoralen, 4-12% of neobavaisoflavone and 2-9% of corylin. The preparing procedure of the invention includes the following steps: soaking the psoraleae medicine into pure water; heating and backstreaming to remove volatile oil; putting the gruffs into alcohol, heating and extracting the medicine; condensing the eluted solution into extractum; separating the extractum with ODS-C18 column using asgradient methanol from low density to high density; drying the eluted solution to get the extract which has definite structures and high content. The medical experiment shows that the extract can substantially inhibit sensitive tumor cell and multidrug resistance tumor cell so that it has obvious anti-tumor function. The invention has proper design and simple preparing method which can be used in preparing medicine that can inhibit sensitive tumor cell and multidrug resistance tumor cell.
Owner:ZHEJIANG UNIV
Effective component of Rabdosia amethystoides and preparation and use thereof
InactiveCN101347501AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidEthyl ester
The present invention relates to an effective component of rabdosia and the preparation method and usage. For the effective component of rabdosia of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract rabdosia; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 20.0 to 24.0min or 24.0 to 28.0min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of vietnamese sophora root and preparation and use thereof
InactiveCN101347497AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidSophora Root
The present invention relates to an effective component of root of subprostrate sophora and the preparation method and usage. For the effective component of root of subprostrate sophora of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract the root of subprostrate sophora; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 36.0 to 40.0min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of artemisia leaves and preparation method thereof
InactiveCN101549001AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidEthyl ester
The invention relates to an effective component of artemisia leaves as well as a preparation method and an application thereof. The preparation process of the effective component of the artemisia leaves comprises the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the artemisia leaves; (2) dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 28.0-32.0 minutes, 36.0-40.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 52.0-60.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Stellera chamaejasme L extract and anti-tumor action thereof
ActiveCN101926844AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAlcoholPolyamide
The invention relates to a Stellera chamaejasme L extract and an anti-tumor action thereof. The Stellera chamaejasme L extract of the invention is extracted by absolute ethyl alcohol to obtain a primary extract; the primary extract is further treated by macroporous resin column chromatography or polyamide column chromatography and then eluted by water and ethyl alcohol to obtain a secondary extract; and in the secondary extract, the secondary extract separated by a macroporous resin column is further treated by polyamide column chromatography and then eluted by water and ethyl alcohol to obtain a tertiary extract.
Owner:INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Effective component of chebula fruit as well as preparation method and use thereof
InactiveCN101433563AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidCyanide compound
The invention relates to an effective component of myrobalam. The preparation process for the effective component comprises the following: step one, using a mixture of ethyl acetate and ethanol as solvent to extract the myrobalam; step two, using the ethanol to extract medicine dregs to obtain extract; step three, obtaining eluent through chromatography of the extract; and step four, using prepared liquid-phase chromatographic gradient to elute the eluent, and collecting the eluent for 48.0 to 52.0 minutes to obtain the effective component, wherein a mobile phase comprises water and methyl cyanide. The invention also discloses a preparation method for the effective component, a pharmaceutically acceptable preparation, and application thereof to preparing anti-tumor medicament.
Owner:TIANJIN TASLY PHARMA CO LTD
Active components of gallnut and preparation method and use thereof
InactiveCN101507737AEfficient removalIncrease contentAnthropod material medical ingredientsAntineoplastic agentsAlcohol ethylGradient elution
The invention discloses an effective component for nutgall, a preparation method of the same and application of the same in resisting tumor. The effective component is obtained through the following steps that: the extraction is performed on the nutgall through a mixture of ethyl acetate and ethanol which is taken as a solvent, and an extracting solution is obtained; the extracting solution is subjected to chromatography in a chromatographic column to obtain an eluent; and the eluent is subjected to gradient elution by preparing liquid-phase chromatography, a mobile phase is water and acetonitrile, and the eluent is collected for 48.0 to 52.0 minutes or 52.0 to 56.0 minutes.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of cynanchum atratum and preparation method thereof
InactiveCN101549000AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsFluid phaseAlcohol ethyl
The invention relates to an effective component of cynanchum atratum as well as a preparation method and an application thereof. The effective component of the cynanchum atratum is prepared according to the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the cynanchum atratum; (2) dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3)the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; and (4) the eluent is eluted by prepared liquid chromatogram gradient and collected for 40.0-44.0 minutes, 44.0-48.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes, 56.0-60.0 minutes and 60.0-64.0 minutes to obtain the effective component by using water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective components of Cacumen Platycladi and preparation method thereof
InactiveCN101550081AEfficient removalIncrease contentOrganic active ingredientsAntineoplastic agentsAcetic acidEthyl ester
The invention relates to effective components of Cacumen Platycladi as well as a preparation method and a purpose thereof. The preparation process comprises the following steps: 1. the mixer of ethyl acetate and ethanol is used as a solvent for extracting Cacumen Platycladi; 2. eluent is obtained from an extracting solution through chromatographic column chromatography; 3. with the moving phase ofwater and acetonitrile, the eluent obtained by the phase color chromatographic gradient elution of a preparation solution is collected for 36.0-40.0 minutes, and the effective components are obtained from the eluent after 44.0-48.0 minutes.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of artemisia leaves and preparation method thereof
InactiveCN101550120AEfficient removalIncrease contentOrganic active ingredientsSugar derivativesSolventChromatographic column
The invention relates to an effective component of artemisia leaves as well as a preparation method and an application thereof. The preparation process of the effective component comprises the following steps: (1 )a mixture of ethyl acetate and ethanol is used as a solvent to extract the artemisia leaves; (2) an extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (3) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 4.0-8.0 minutes, 8.0-10.0 minutes, 16.0-20.0 minutes, 20.0-24.0 minutes, 24.0-28.0 minutes, 28.0-32.0 minutes and 36.0-40.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of airpotato yam as well as preparation method and use thereof
InactiveCN101347567AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidElution
The invention relates to effective components of an airpotato yam rhizome, a preparation method and the application thereof. The process for preparing the effective components of an airpotato yam rhizome comprises the following steps: step 1, the airpotato yam rhizome is extracted by the mixture of ethyl acetate and ethanol, which is used as a solvent; step 2, medicine residues are extracted by ethanol to obtain extracting solution; step 3, extracting solution is treated with column chromatography to obtain elution solution; step 4, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 10.8-13.8 min is collected to obtain the effective components.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of schisandra chinensis, preparation method and use thereof
InactiveCN101428062AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsChromatographic columnSolvent
The invention provides an active component of schisandra fruit. The active component of the schisandra fruit is prepared as follows: step 1, the mixture of ethyl acetate and ethanol is taken as the solvent for extracting the schisandra fruit; step 2, the eluent is obtained from an extracting solution through chromatographic column chromatography; and step 3, the eluent is prepared by liquid chromatography gradient elution, the mobile phase comprises water and acetonitrile, and the active component is obtained after the eluent is collected for 48.0-52.0 min. The active component of the schisandra fruit and the pharmaceutical composition prepared by the component are applied to the anti-tumor aspect.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of Atractylodes macrocephala and preparation and use thereof
InactiveCN101347484AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidAtractylodes macrocephala
The present invention relates to an effective component of Rhizoma Atractylodis and the preparation method and usage. For the effective component of Rhizoma Atractylodis of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Rhizoma Atractylodis; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 28.1 to 33.9min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of hedyotis diffusa and method of preparing the same and application
InactiveCN101129521AEfficient removalIncrease contentQuinone separation/purificationAntineoplastic agentsChromatographic separationHydroxyanthraquinone
The invention provides the effective constituents of oldenlandia, which mainly contain two compounds of 2-methoxyl-3-hydroxyanthraquinone (A) and 2-methyl-3-hydroxyanthraquinone (B), wherein the content of A is 55-75%, the content of B is 15-25%. The constituents are obtained through steps of heating, extracting, concentrating, separating with silica gel column, eluting, concentrating and drying the eluent, continuously conducting liquid phase chromatographic separation, collecting the solution, concentrating and drying the solution. The effective constituents can be used for treating and preventing tumors.
Owner:ZHEJIANG UNIV
Effective component of cortex dictamni, preparation method and use thereof
InactiveCN101428083AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidActive component
The invention relates to an active component of cortex dictamni radicis, and a preparation method and the application thereof. The preparation process comprises the following steps: step 1, the mixture of ethyl acetate and ethanol is taken as a solvent for extracting the cortex dictamni radicis; step 2, the eluent is obtained from an extracting solution through chromatographic column chromatography; and step 3, the eluent is prepared by liquid chromatography gradient elution, the mobile phase includes water and acetonitrile, and the active component is obtained after the eluent is collected for 24.0-28.0 min, 28.0-32.0 min, 32.0-36.0 min, 48.0-52.0 min, 52.0-56.0 min and 60.0-64.0 min.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of Spatholobus stem and preparation and use thereof
InactiveCN101347496AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidGradient elution
The present invention relates to an effective component of leatherleaf millettia and the preparation method and usage. For the effective component of leatherleaf millettia of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract leatherleaf millettia; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 32.6 to 40.8min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of cogongrass rhizome and preparation method thereof
InactiveCN101549111AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidEthyl ester
The invention relates to an effective component of cogongrass rhizome as well as a preparation method and an application thereof. The preparation process of the effective component comprises the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the cogongrass rhizome; (2) an extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (3) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 56.0-60.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of gamene and preparation and use thereof
InactiveCN101347534AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidElution
The invention relates to an effective component of madder root, a preparation method and the application thereof; the preparation process of the effective component of madder root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting madder root; step 2, herb residue is extracted by ethanol to obtain extract; step 3, the extract is processed with chromatographic column chromatography to obtain eluate; step4, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 48.0-52.0 min is collected to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of Schisandra chinensis as well as preparation method and use thereof
InactiveCN101433587AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsGradient elutionEthyl acetate
The invention relates to an effective composition of fructus schizandrae. The preparation process comprises the following: step one: a mixture of acetic ether and ethanol is used as a solvent and carries out extraction on the fructus schizandrae; step two: an extraction solution is subjected to column chromatography to obtain an eluant; and step three: the eluant prepared through liquid phase chromatographic gradient elution uses water and acetonitrile as a mobile phase; and the eluant is collected for 44.0 to 48.0 minutes to obtain the effective composition. The invention also provides a method for preparing the effective composition and application thereof. The effective composition is first obtained through the method and is first applied to the drug effect screening of various tumor cell strains; and as the effective composition is precise and has definite content, a convenient preparation process and good activity, the effective composition is suitable to be developed into a novel antitumor Chinese medicine.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of chebula fruit, preparation method and use thereof
InactiveCN101428043AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidActive component
The invention relates to an active component of a myrobalan fruit, and a preparation method and the application thereof. The preparation process comprises the following steps: firstly, a mixture of ethyl acetate and ethanol is taken as a solvent for extracting the myrobalan fruit; secondly, the eluent is obtained from an extracting solution through chromatographic column chromatography; and thirdly, the eluent is prepared by liquid chromatography gradient elution, the mobile phase includes water and acetonitrile, and the active component is obtained after the eluent is collected for 24.0-28.0min, 28.0-32.0min, 32.0-36.0min, 48.0-52.0min, 52.0-56.0min and 60.0-64.0min.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of creat and preparation and use thereof
InactiveCN101347473AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidEthyl ester
The present invention relates to an effective component of Common Andrographis Herb and the preparation method and usage. For the effective component of Common Andrographis Herb of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Common Andrographis Herb; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 25.5 to 33.8min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of red paeonia and preparation and use thereof
InactiveCN101347518AEfficient removalIncrease contentSugar derivativesSugar derivatives preparationAcetic acidElution
The invention relates to an effective component of red peony root, a preparation method and application thereof; the preparation process of the effective component of red peony root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting red peony root; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 28.6-32.2 min is collected to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of morinda officinalis and preparation method thereof
InactiveCN101549036AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsChromatographic columnSolvent
The invention relates to an effective component of morinda officinalis as well as a preparation method and an application thereof. The preparation process of the effective component of the morinda officinalis comprises the following steps: (1)a mixture of ethyl acetate and ethanol is used as a solvent to extract the morinda officinalis; (2)dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3)the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4)the eluent is eluted by prepared liquid chromatogram gradient and collected for 28.0-32.0 minutes and 52.0-56.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Salvia miltiorrhiza valid component, preparing method and application
InactiveCN101199593AEfficient removalIncrease contentOrganic active ingredientsAntineoplastic agentsElutionSilica gel
The invention provides effective components of radix salviae miltiorrhizae, mainly comprising TrijuganoneC as A compound, 7-Hydroxy-8, 13-abietadiene-11, 12-dione as B compound and Dihydrotanshinone I as C compound with respective content of 40 to 50 percent, 30 to 40 percent and 3 to 10 percent. The effective components can be obtained through following procedures: heating extraction, condensing, silica gel column separation, elution, condensing and drying of the eluted solution, liquid chromatography separation, solution collecting and condensing and drying of the solution. The effective components can be used in the preparation of drugs for treating and preventing tumors. With reasonable design, the invention can be quickly obtained and can be more favorable for quality control of the drugs.
Owner:ZHEJIANG UNIV
Effective component of rabdosia and preparation and use thereof
InactiveCN101347500AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidGradient elution
The present invention relates to an effective component of rabdosia rubescens and the preparation method and usage. For the effective component of rabdosia rubescens of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract rabdosia rubescens ; step 2: the extract goes column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 15.5 to 19.6min so as to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of phragmites communis trin, preparation method and use thereof
InactiveCN101428120AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsAcetic acidEthyl ester
The invention relates to a reed rhizome effective component, a preparation method thereof and a use, and the preparation process comprises the following steps: step 1: mixture of ethyl acetate and ethanol is taken as a solvent to extract reed rhizome; step 2: eluent is obtained by leading extract liquid to pass through a chromatographic column for chromatography; and step 3: the eluent which is obtained by using the liquid-phase chromatographic gradient elution with the mobile phase of water and acetonitrile is used for collecting the eluent at 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 60.0-64.0 minutes for obtaining the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of cogongrass rhizome and preparation method thereof
InactiveCN101550072AEfficient removalIncrease contentHydroxy compound active ingredientsOrganic compound preparationAcetic acidEthyl ester
The invention relates to an effective component of cogongrass rhizome as well as a preparation method and an application thereof. The preparation process of the effective component of the cogongrass rhizome comprises the following steps: (1)a mixture of ethyl acetate and ethanol is used as a solvent to extract the cogongrass rhizome; (2) dregs of a decoction are extracted with the ethanol so as toobtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogra m gradient and is collected for 4.0-8.0 minutes, 8.0-12.0 minutes, 20.0-24.0 minutes, 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 36.0-40.0 minutes, 40.0-44.0 minutes, 44.0-48.0 minutes; 48.0-52.0 minutes, 52.0-56.0 minutes, 56.0-60.0 minutes and 60.0-64.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.
Owner:TIANJIN TASLY PHARMA CO LTD
Method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity, and use
InactiveCN102824382ASimple extraction and separation processThe chemical composition is simple and clearOrganic active ingredientsDigestive systemAcetic acidReflux
The invention relates to a method for preparing Ligusticum wallichii lactone extract having anti-liquid-accumulation activity and a use, especially to a Ligusticum wallichii extract containing 30 to 90 % of ligustilide. The extract is prepared by the following method comprising smashing the Ligusticum wallichii medicinal materials, immersing in 70 to 99 % ethanol, e.g. 75 to 98 %, extracting by reflux for 1 to 4 times, combining extract, condensing, extracting by using petroleum ether, recovering the solvent of the combined extract till dryness to get a petroleum ether extract; extracting a part of the extract by using the petroleum ether, adding normal phase silica gel to stir, going through a normal phase silica gel column, eluting by using the petroleum ether and an ethyl acetate solution with a volume ratio of 50 : 0.5-5 as eluents, collecting the eluates, and condensing to get the Ligusticum wallichii lactone extract.
Owner:TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
Effective component of schisandra chinensis, preparation method and use thereof
InactiveCN101428064AEfficient removalIncrease contentAntineoplastic agentsPlant ingredientsGradient elutionSolvent
The invention relates to an active component of schisandra fruit, and a preparation method and the application thereof. The preparation process comprises the following steps: step 1, the mixture of ethyl acetate and ethanol is taken as a solvent for extracting the schisandra fruit; step 2, the eluent is obtained from an extracting solution through chromatographic column chromatography; and step 3, the eluent is prepared by liquid chromatography gradient elution, the mobile phase includes water and acetonitrile, and the active component is obtained after the eluent is collected for 24.0-28.0 min, 28.0-32.0 min, 32.0-36.0 min, 48.0-52.0 min, 52.0-56.0 min and 60.0 -64.0 min.
Owner:TIANJIN TASLY PHARMA CO LTD
Effective component of selfheal and preparation and use thereof
InactiveCN101347502AEfficient removalIncrease contentOrganic chemistryAntineoplastic agentsAcetic acidElution
The invention relates to an effective component of prunella vulgaris, a preparation method and application thereof; the preparation process of the effective component of prunella vulgaris in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting prunella vulgaris; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 24.0-28.0 min or 28.0-32.0 min is collected to obtain the effective component.
Owner:TIANJIN TASLY PHARMA CO LTD
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