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Effective component of creat and preparation and use thereof

An active ingredient, andrographis paniculata technology, applied in organic chemistry, drug combination, medical raw materials derived from angiosperm subphylum, etc., can solve problems such as side reactions, involving normal cells, etc.

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of creat and preparation and use thereof
  • Effective component of creat and preparation and use thereof
  • Effective component of creat and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 andrographis paniculata effective components

[0083] Take 250 g of Andrographis paniculata, grind it, add ethyl acetate and ethanol (1:1), heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract was concentrated into extract to obtain 15.5g, the extract and silica gel were mixed as a sample, and separated with a normal phase silica gel column, at first using sherwood oil and ethyl acetate (50:1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 1.7 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18 ; 21.2mm × 250mm), the mobile phase is water (A) and acetonitrile (B), and the gradient el...

Embodiment 2

[0086] The analysis of embodiment 2 Andrographis paniculata effective components

[0087] (1) HPLC-ELSD analysis of the Andrographis paniculata effective component of embodiment 1

[0088] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5...

Embodiment 3

[0091] Embodiment 3 andrographis paniculata active ingredient preparation

[0092] Take 0.5 g of the effective components of Andrographis paniculata in Example 1 and mix them evenly with 10.5 g of polyethylene glycol-6000, heat and melt, move the material to drip irrigation, drop the drug solution into liquid paraffin at 6-8°C, and remove the oil , made 400 dropping pills.

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PUM

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Abstract

The present invention relates to an effective component of Common Andrographis Herb and the preparation method and usage. For the effective component of Common Andrographis Herb of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Common Andrographis Herb; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 25.5 to 33.8min so as to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Andrographis paniculata, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained...

Claims

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Application Information

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IPC IPC(8): A61K36/19A61P35/00C07D407/06A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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