The invention provides a construction method of an HPLC (
High Performance Liquid Chromatography)
fingerprint spectrum of a
salvia miltiorrhiza and radix puerariae depression relieving
drug. The method comprises the steps of preparing a sample solution, preparing a reference substance solution, preparing efficient liquid chromatogram condition, and detecting to obtain the
HPLC fingerprint spectrum of the
salvia miltiorrhiza and radix puerariae depression relieving
drug; 17 characteristic peaks are detected from the
HPLC fingerprint spectrum of the
salvia miltiorrhiza and radix puerariae depression relieving
drug, including 14 characteristic peaks of
salvia miltiorrhiza, and 3 characteristic peaks of radix puerariae. According to the construction method, the HPLC-DAD-TOF / MS serial connecting technology is carried out to point out each chromatographic peak of the
HPLC fingerprint spectrum of the
salvia miltiorrhiza and radix puerariae depression relieving drug; meanwhile, the one-testing and multi-evaluation method is carried out to detect the content of main effective ingredients of the
salvia miltiorrhiza and radix puerariae depression relieving drug, as well as detecting the content limit standard; therefore, the construction method of the
fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug has the advantages that the qualitative perforamnce and quantification performance are combined to construct a multi-information
fingerprint spectrum with clear spectrum, clear ingredients and controllable content, thus the quality of the salvia miltiorrhiza and radix puerariae depression relieving drug can be comprehensively reflected, and the
quality control method of the salvia miltiorrhiza and radix puerariae depression relieving drug can be effectively improved.