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Preparation method for efficiently extracting separating high-purity flavone components from ginkgo leaf

A technology of ginkgo biloba leaves and high purity, applied in the field of medicine, can solve the problems of affecting the measurement results, long hydrolysis time, incomplete hydrolysis and the like, and achieves the effects of abundant resources, good reproducibility and convenient operation.

Inactive Publication Date: 2013-05-22
北京华润高科天然药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the hydrolysis method is used to determine the content of ginkgo flavonoids. The hydrolysis time is long, time-consuming and laborious, and the hydrolysis conditions suc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1:

[0031] Ginkgo biloba raw medicinal material with a water content of 6% was crushed, weighed 10 kilograms quantitatively, placed in a 200L extraction tank, added with a weight of 100 kilograms of 40% ethanol solution, soaked and extracted 3 times at 30°C for 2 days each time, combined the extracts, The extract was concentrated to obtain 3.6L extract with a relative density of 1.21.

[0032] Add ethanol to the extract to make the volume concentration of ethanol reach 60%, and stir for 30 minutes, refrigerate and stand for 24 hours at 4°C, filter, concentrate the filtrate to extract with a relative density of 1.15; add ethanol to make the volume concentration of ethanol reach 80 %, and fully stirred for 30 minutes, refrigerated at 4°C and allowed to stand for 48 hours to filter. The filtrate was concentrated and volatilized to remove ethanol, and concentrated to obtain 2.8L extract with a relative density of 1.17.

[0033] Add 16.8L of water to the alcohol-precipita...

Example Embodiment

[0036] Example 2:

[0037] The ginkgo biloba raw medicinal material with a water content of 2% is crushed, 2 kg is quantitatively weighed, placed in a 50L extraction tank, added with a 20% ethanol solution weighing 18 kg, soaked and extracted at 45°C for 5 times, each time for 1 day. The extract was concentrated to obtain 1.1L extract with a relative density of 1.15.

[0038] Add ethanol to the extract to make the volume concentration of ethanol reach 50%, and stir for 30 minutes, refrigerate and stand for 36 hours at 4°C, filter, and concentrate the filtrate to extract with a relative density of 1.12; then add ethanol to make the volume concentration of ethanol reach 85 %, and fully stirred for 30 minutes, refrigerated at 4°C for 24 hours to filter, the filtrate was concentrated and volatilized to remove ethanol, and concentrated to obtain 0.7L of extract with a relative density of 1.24.

[0039] Add 7L of water to the alcohol-precipitated extract according to the volume ratio of 1...

Example Embodiment

[0042] Example 3:

[0043] The ginkgo biloba raw medicinal material with 9% water content is crushed, 25 kg is quantitatively weighed, and it is placed in a 500L extraction tank and added with a weight of 300 kg 50% ethanol solution. The extract is soaked and extracted twice at 50°C for 5 days each time. The extract was concentrated to obtain 7.9L extract with a relative density of 1.23.

[0044] Add ethanol to the extract to make the volume concentration of ethanol reach 70%, and stir for 30 minutes, refrigerate and stand for 15 hours at 4°C, filter, and concentrate the filtrate to an extract with a relative density of 1.14; then add ethanol to make the volume concentration of ethanol reach 85 %, and fully stirred for 30 minutes, 4°C refrigerated and stood for 40 hours to filter, the filtrate was concentrated and volatilized to remove ethanol, and concentrated to obtain 4.6L of extract with a relative density of 1.19.

[0045] Add 36.8L of water to the alcohol-precipitated extract ...

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Abstract

The invention provides a preparation method for efficiently extracting separating high-purity flavone components from ginkgo leaf. The main components are kaempferol-3-O-2'', 6''-dirhamnosylglucoside, rutin, quercetin glucosyl rhamnoside, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, quercetin p-coumaroyl glucose rhamnoside and kaempferol p-oumaroyl glucose rhamnoside. The preparation method comprises the following steps: pulverizing the medicinal material ginkgo leaf, adding an alcohol water solution to carry out extraction, concentrating, carrying out nonpolar macroporous adsorbent resin column chromatography on the filtrate, carrying out two-dimensional separation with a novel silica gel substrate bonded material, eluting with a methanol solution, collecting the eluate, concentrating, and drying to obtain the gingko flavone components. The invention enhances the target component content, and obtains the flavone components with definite content. The extraction and separation process has the advantages of high repetitiveness and favorable operability, and is suitable for large-scale preparation of flavone components by separation from ginkgo leaf. The flavone components can be used as a raw material for Shuxuening injections.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to an efficient extraction, separation and preparation method of high-purity flavonoid components in ginkgo leaves. Background technique [0002] Ginkgo is a gymnosperm of the Ginkgo family, also called ginkgo tree and Gongsun tree. It is a precious tree species unique to my country and the oldest tree species in the world. It is called "living fossil" and is widely distributed in my country. Ginkgo biloba has been used as medicine in my country for more than 5,000 years. Modern medical research has found that Ginkgo biloba has the functions of lowering blood lipids, scavenging oxygen free radicals, enhancing nervous system function, regulating rapid levels, anti-inflammatory and anti-allergic effects. As health food and botanical medicine, it is widely used to prevent memory loss, dementia and cardiovascular and cerebrovascular diseases. [0003] The chemical composition of Gi...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61P9/00A61P25/00A61P25/28A61K127/00
Inventor 丰加涛付冬梅王海涛冯志琼
Owner 北京华润高科天然药物有限公司
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