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Effective component of vietnamese sophora root and preparation and use thereof

An active ingredient, Shandou technology, applied in medical preparations containing active ingredients, organic chemistry, drug combination, etc., can solve problems such as side effects and normal cells

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of vietnamese sophora root and preparation and use thereof
  • Effective component of vietnamese sophora root and preparation and use thereof
  • Effective component of vietnamese sophora root and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] The preparation of embodiment 1 mountain bean root effective components

[0115] Take 250 g of mountain bean root medicinal material, grind it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 9.8g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 1.5 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18 ; 21.2mm × 250mm), the mobile phase is water (A) and acetonitrile (B), and...

Embodiment 2

[0118] The analysis of embodiment 2 Shandou root active components

[0119] The HPLC-ELSD joint analysis to the effective component of the mountain bean root of embodiment 1

[0120] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2%...

Embodiment 3

[0123] Embodiment 3 Shandou root active ingredient preparation

[0124] Take 0.5 g of the active component of the mountain bean root in Example 1 and mix it evenly with 10.5 g of polyethylene glycol-6000, heat and melt it, and move it to the drip irrigation after the material is formed, and drop the medicine liquid into the liquid paraffin at 6-8 °C, Degreasing, made 400 dropping pills.

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PUM

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Abstract

The present invention relates to an effective component of root of subprostrate sophora and the preparation method and usage. For the effective component of root of subprostrate sophora of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract the root of subprostrate sophora; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 36.0 to 40.0min so as to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from the mountain bean root, a preparation, a preparation method and an application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound o...

Claims

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Application Information

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IPC IPC(8): A61K36/489A61P35/00C07D311/22C07D311/30C07D311/32C07D311/36C07D493/04A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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