Method for quickly determining anthocyanin in seed coat of black peanut by using UPLC/MS/MS (Ultra Performance Liquid Chromatography/tandem Mass Spectrometry)
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for rapid determination of black peanut seed coat, applied in the field of detection, can solve the problems of inability to determine the type and content of anthocyanins, inability to identify the structure of unknown substances, etc., and achieve the effect of rapid extraction
Inactive Publication Date: 2013-08-07
山东省农业科学院中心实验室
View PDF5 Cites 12 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The method of separation and purification is mainly chromatography, including paper chromatography (PC), thin layer chromatography (TLC), column chromatography (CC), and high performance liquid c
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0040] In order to clarify the understanding of the features of the present invention, the present invention will be further described below with reference to the embodiments.
[0041] The technical solutions of the present invention are further described below with reference to the embodiments.
[0042] 1. Instruments and samples
[0043] 1.1. Instrument: Ultra-high performance liquid chromatography system (ACQUITYUltraPerformanceLCWaters company) with mass spectrometry (MicromassQuattroUltimaIMPT mass spectrometer Waters company), N-EVAP TM 112 nitrogen blowing instrument, WATERS solid phase extraction instrument, MSC 18 (2.1mm×150mmI.D.,3.5μm) chromatographic column.
[0044] 1.2. Sample: black peanut
[0045] 2. Methods and Results
[0046] 2.1. Extraction of anthocyanins from black peanut seed coat
[0047] Weigh 0.20g black peanut seed coat sample into a 50mL stoppered plastic centrifuge tube, add 10mL of 90% methanol aqueous solution, vortex for 1min, ultrasonicall...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for quickly determining anthocyanin in a seed coat of a black peanut by using UPLC/MS/MS (Ultra Performance Liquid Chromatography/tandem Mass Spectrometry), belonging to the technical field of detection. The method comprises the following steps of (1) extracting anthocyanin in the seed coat of the black peanut, (2) purifying anthocyanin in the seed coat of the black peanut, and (3) analyzing and determining anthocyanin in the seed coat of the black peanut. With the adoption of the method, anthocyanin in the black peanut can be extracted quickly and fully, so that the kind and the content of different anthocyanin can be determined precisely; the method has an important significance in revealing a material basis and an action mechanism of an activity function of anthocyanin; and a theoretical foundation is provided for developing the peanut variety rich in anthocyanin content and improving the nutritional quality and a health-care function of the peanut.
Description
technical field [0001] The invention relates to the technical field of detection, in particular to a method for rapidly determining anthocyanins in black peanut seed coat by utilizing UPLC / MS / MS. Background technique [0002] Anthocyanins are a kind of natural water-soluble pigments, which are widely present in the cell sap of the roots, stems, leaves, flowers, fruits and other organs of plants, which makes them appear red, blue, purple and other colors. Anthocyanins are a class of polyphenolic compounds composed of anthocyanins and sugars through glycosidic bonds. The basic structure of anthocyanin is 3,5,7-trihydroxy-2-phenylbenzopyran. There are more than 20 known anthocyanins, but there are mainly 6 kinds in plants, namely cyanidin, Geranium pigment, delphinium pigment, peony pigment, morning glory pigment, mallow pigment. Anthocyanins have certain nutritional and health care effects, such as antioxidant, anti-mutation, anti-tumor and anti-arteriosclerosis, etc., and h...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more