Microbial fermented composite antibiotic-free feed containing natural antibacterial components
A natural antibacterial, antibacterial feed technology, applied in the direction of animal feed, animal feed, animal feed molding or processing, etc., can solve problems such as can not replace antibiotics, biological fermented feed does not have antibacterial effect, can not solve the problem of antibiotic use and so on
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Embodiment 1
[0126] Preparation of embedded materials of natural antibacterial components
[0127] (1) Extraction of active ingredients
[0128] Weigh the following medicinal materials in the following proportions by weight: 60 parts of oregano, 30 parts of thyme, 25 parts of cinnamon, 10 parts of honeysuckle, 15 parts of Houttuynia cordata, 10 parts of Astragalus, dust and impurities, and crushed to a length of less than 2cm, a total of Scrap 3300g, with 20L distilled water and 2L absolute ethanol as the solvent, flash extraction for 2min in a flash extractor, repeat the extraction 3 times, control the extraction temperature to 60℃, filter, and collect the filtrate; filter residue with 5 times the mass Disperse in 90% ethanol aqueous solution, extract with ultrasonic vibration at 50℃ and 300W for 45min, filter, dry the filtered medicinal material filter residue, pulverize, and save for later use (as one of the fermentation raw materials); extract the filtrate obtained by ultrasonic vibration ...
Embodiment 2
[0132] Strain cultivation and compound bacterial liquid preparation
[0133] (1) Strain culture
[0134] The suitable strains of the present invention are conventional feedable strains or edible strains.
[0135] The selected strains in this embodiment include: selected from Lactobacillus platarum (Lactobacillus platarum)
[0136] The lactic acid bacteria, Bacillus subtilis, complex yeast (any combination of Candida utilis and Saccharomyces cerevisiae), and Enterococcus faecalis. The strains are selected according to the feedable strains in the "Catalogue of Feed Additives" (promulgated by the Ministry of Agriculture); specifically, it is preferably Bacillus subtilis
[0137] CCTCC NO: M 2011328, Lactobacillus plantarum CICC NO: 21790 and Candida utilis CICC NO: 31430 etc.
[0138] S1: Cultivation of strains
[0139] Lactic acid bacteria and Enterococcus faecalis culture: Lactobacillus and Enterococcus faecalis are inoculated into slant medium (slant medium: beef extract 0.4%, peptone ...
Embodiment 3A
[0151] The preparation of mixed secondary fermentation material A includes the primary fermentation of the fermentation base material and the mixed secondary fermentation of the mixture. The specific steps are as follows:
[0152] Bottom: S1: select the composite bacterial liquid and Enterococcus faecalis bacterial liquid prepared in the above examples; S2: selection and preparation of fermentation raw materials
[0153] The fermentation raw material is composed of fermentation base material, medicinal material filter residue after extracting natural antibacterial components, and fermentation assistant. The fermentation base material is composed of defatted soybean meal, bran, corn germ meal, corn husk and dephenolic cotton meal. The specific composition of the fermentation raw materials is: 40 parts of defatted soybean meal, 20 parts of wheat bran, 25 parts of corn germ meal, 5 parts of dephenolic cotton meal; 3 parts of natural antibacterial extract filter residue; auxiliary: 0.6 ...
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