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Effective component of artemisia leaves and preparation method thereof

A technology of effective components and argyi leaves, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve the problems of involving normal cells and side effects, etc.

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of artemisia leaves and preparation method thereof
  • Effective component of artemisia leaves and preparation method thereof
  • Effective component of artemisia leaves and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1 Preparation of Artemisia argyi Effective Components

[0116] The preparation method of the effective components of Artemisia argyi of the present invention comprises the following steps: adding ethyl acetate and ethanol (1:1) after crushing Artemisia argyi medicinal materials, heating and refluxing for 1 hour, extracting twice, combining the filtrates to obtain an extract; concentrating the extract into The extract was dissolved in ethanol and loaded, passed through the ODS-C18 column, firstly, 30% EtOH (5BV; 1BV≈250ml) was used as the mobile phase to obtain eluent I, and then 95% EtOH (5BV) was replaced as the mobile phase, The eluent II is obtained, and the eluent II is concentrated and dried to obtain a sample; the sample obtained by continuing to separate the preparative liquid chromatography; the separation condition of the preparative chromatography: the chromatographic column is a preparative column Zorbax SB-C18; 21.2mm×250mm, flow The phases are water...

Embodiment 2

[0120] Example 2 Analysis of the active components of Folium Artemisiae Argyi

[0121] The HPLC-ELSD joint analysis of the effective components of Folium Artemisiae Argyi of Example 1

[0122] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A wa...

Embodiment 3

[0125] Embodiment 3 Artemisia argyi effective component preparation

[0126] Get the Artemisia argyi active ingredient 1, 2, 3, 4, 5, 6 or 7 of Example 1, mix 0.5g with 10.5g polyethylene glycol-6000, heat and melt, and move to the drip irrigation after the chemical material, Drop the drug solution into liquid paraffin at 6-8°C, remove oil, and make 400 dropping pills.

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PUM

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Abstract

The invention relates to an effective component of artemisia leaves as well as a preparation method and an application thereof. The preparation process of the effective component comprises the following steps: (1 )a mixture of ethyl acetate and ethanol is used as a solvent to extract the artemisia leaves; (2) an extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (3) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 4.0-8.0 minutes, 8.0-10.0 minutes, 16.0-20.0 minutes, 20.0-24.0 minutes, 24.0-28.0 minutes, 28.0-32.0 minutes and 36.0-40.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

Description

Technical field: [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from leaves of mugwort, a preparation and a preparation method and use thereof. Background technique: [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained fro...

Claims

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Application Information

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IPC IPC(8): C07D307/80C07D311/30C07D493/10C07H15/203A61K31/352A61K36/282A61P35/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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