Effective component of cogongrass rhizome and preparation method thereof

An active ingredient, the technology of Imperatae coronata, applied in the direction of medical preparations containing active ingredients, preparation of organic compounds, effective ingredients of hydroxyl compounds, etc., can solve problems involving normal cells, side effects, etc.

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy,

Method used

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  • Effective component of cogongrass rhizome and preparation method thereof
  • Effective component of cogongrass rhizome and preparation method thereof
  • Effective component of cogongrass rhizome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Example 1 Preparation of Effective Components of Imperata cylindrica Root

[0135] Imperata cylindrica roots are crushed and passed through a 20-mesh sieve. Add 8 times the amount of ethyl acetate: ethanol = 1:1, heat and reflux for 1 hour, extract twice, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, and extract Two times, the filtrates were combined to obtain the extract. After the extract was concentrated, the sample was dissolved in 5% ethanol and passed through the ODS-C18 column. First, 1250ml of 5% ethanol was used as the mobile phase, and then changed to 1250ml of 50% ethanol as the mobile phase. Obtain the eluent, and continue to separate the eluent by preparative liquid chromatography. The separation conditions: the chromatographic column is an Agilent preparative column (ZorbaxSB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0136]

[0137] The flow rate is...

Embodiment 2

[0138] Example 2 Analysis of Effective Components of Imperata cylindrica Root

[0139] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm×150mm, 5μm); using gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid in water, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid The aqueous solution and mobile phase B are 50% 0.2% glacial acetic acid in acetonitrile; at 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid in water, and mobile phase B is 95% 0.2% glacial acetic acid in acetonitrile; 35 At minutes, the mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, and the mobile phase B is 95% 0.2% glacial acetic acid in acetonitrile. Solution flo...

Embodiment 3

[0142] Example 3 The effective component preparation of Imperata cylindrica

[0143] Take the effective components 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 or 13, of the effective components of Imperata cylindrica in Example 1, and mix 0.5g and 10.5g polyethylene glycol-6000 uniformly. After heating and melting, the material is transferred to drip irrigation, and the medicine is dropped into liquid paraffin at 6-8°C, and the oil is removed to obtain 400 drip pills.

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Abstract

The invention relates to an effective component of cogongrass rhizome as well as a preparation method and an application thereof. The preparation process of the effective component of the cogongrass rhizome comprises the following steps: (1)a mixture of ethyl acetate and ethanol is used as a solvent to extract the cogongrass rhizome; (2) dregs of a decoction are extracted with the ethanol so as toobtain an extracting solution; (3) the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4) the eluent is eluted by prepared liquid chromatogra m gradient and is collected for 4.0-8.0 minutes, 8.0-12.0 minutes, 20.0-24.0 minutes, 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 36.0-40.0 minutes, 40.0-44.0 minutes, 44.0-48.0 minutes; 48.0-52.0 minutes, 52.0-56.0 minutes, 56.0-60.0 minutes and 60.0-64.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from the root of Imperata cylindrica, a preparation, and a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumors are currently the most serious type of disease endangering human health. At present, the treatment of malignant tumors in the industry is mainly surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genetic toxicity of botanical drugs is not obvious. Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation. Moreover, Chinese medicine also plays an important role in the adjuvant treatment of tumors. Paclitaxel is a typical natural compoun...

Claims

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Application Information

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IPC IPC(8): C07C43/23C07C41/58C07D491/16C07D493/04C07C35/44C07C29/74C07D311/16A61K31/075A61K31/437A61K31/341A61K31/045A61K31/366A61K36/899A61P35/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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