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Effective component of phragmites communis trin, preparation method and use thereof

An effective component, reed rhizome technology, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-05-13
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of phragmites communis trin, preparation method and use thereof
  • Effective component of phragmites communis trin, preparation method and use thereof
  • Effective component of phragmites communis trin, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Embodiment 1 Preparation of effective components of reed rhizome

[0114] Take 250 g of reed rhizome, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. Concentrate the extract into extract to obtain 8.6g, dissolve and load the sample with ethanol, and pass through the ODS-C18 column. First, use 1250ml of 30% ethanol as the mobile phase to obtain the eluate, discard it, and then replace it with 1250ml of 95% ethanol as the mobile phase. phase to obtain eluent II, and concentrate and dry the eluent II to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography; separation conditions for preparative chromatography: the chromatographic column is a preparative column Zorbax SB-C18; 21.2mm×250mm , the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows:

[0115]

[0116] The flow rate is 10ml / min, the column...

Embodiment 2

[0117] Example 2 Analysis of effective components of reed rhizome

[0118] The HPLC-ELSD joint analysis to the reed rhizome effective component of embodiment 1

[0119] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic...

Embodiment 3

[0122] Example 3 Reed Rhizome Active Component Preparation

[0123] Take the effective component of reed rhizome of Example 1, mix 0.5g with 10.5g of polyethylene glycol-6000 evenly, heat and melt, move the material to the drip irrigation after the material, drop the medicine liquid into the liquid paraffin at 6-8°C, Degreasing, made 400 dropping pills.

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PUM

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Abstract

The invention relates to a reed rhizome effective component, a preparation method thereof and a use, and the preparation process comprises the following steps: step 1: mixture of ethyl acetate and ethanol is taken as a solvent to extract reed rhizome; step 2: eluent is obtained by leading extract liquid to pass through a chromatographic column for chromatography; and step 3: the eluent which is obtained by using the liquid-phase chromatographic gradient elution with the mobile phase of water and acetonitrile is used for collecting the eluent at 24.0-28.0 minutes, 28.0-32.0 minutes, 32.0-36.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes and 60.0-64.0 minutes for obtaining the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from reed rhizome, a preparation, a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61P35/00A61K125/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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