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Effective component of selfheal and preparation and use thereof

An effective component, the technology of Prunella vulgaris, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of selfheal and preparation and use thereof
  • Effective component of selfheal and preparation and use thereof
  • Effective component of selfheal and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Example 1 Preparation of effective components of Prunella vulgaris

[0142] Take 250 g of Prunella vulgaris herbal material, pulverize it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrate to obtain the extract. The extract was concentrated into an extract to obtain 16.6g. The extract and silica gel were mixed and separated by a normal phase silica gel column. First, petroleum ether and ethyl acetate (50:1) were used as mobile phases to obtain eluent I. Then, chloroform and methanol (10:1) were changed as mobile phases to obtain eluent II, which was concentrated and dried to obtain 5.0 g of sample. Use preparative liquid chromatography to continue to separate the obtained sample. Separation conditions of preparative chromatography: chromatographic column is Agient preparative column (Zorbax SB-C 18 ; 21.2mm×250mm), the mobile phase is water (A) and acetonitrile (B), the gradient elution procedure is as follows:

[0143] ...

Embodiment 2

[0145] Example 2 HPLC-ELSD analysis of effective components of Prunella vulgaris

[0146] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm×150mm, 5μm); using gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B phase is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid in water, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid The aqueous solution and mobile phase B are 50% 0.2% glacial acetic acid in acetonitrile; at 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid in water, and mobile phase B is 95% 0.2% glacial acetic acid in acetonitrile; 35 At minutes, the mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, and the mobile phase B is 95% 0.2% glacial acetic acid in acetonitrile. So...

Embodiment 3

[0149] Example 3 Prunella vulgaris effective component preparation

[0150] Take the effective component 1 or component 2 of Prunella vulgaris in Example 1, mix 0.5g and 10.5g polyethylene glycol-6000 uniformly, heat and melt, and transfer the material to drip irrigation, and the medicine drops to 6-8. In liquid paraffin at ℃, the oil was removed to prepare 400 drop pills.

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Abstract

The invention relates to an effective component of prunella vulgaris, a preparation method and application thereof; the preparation process of the effective component of prunella vulgaris in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting prunella vulgaris; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 24.0-28.0 min or 28.0-32.0 min is collected to obtain the effective component.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Prunella vulgaris, a preparation, and a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumors are currently the most serious type of disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genetic toxicity of botanical drugs is not obvious. Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation. Moreover, Chinese medicine also plays an important role in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained ...

Claims

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Application Information

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IPC IPC(8): A61K36/536A61P35/00C07C61/28A61K131/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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